Pharmacokinetic study of the complication of<italic style="font-style: italic"> Ephedra sinica </italic>and<italic style="font-style: italic"> Prunus armeniaca</italic> in bronchial asthma model rats

CHEN Linlin; XIE Jingen; FAN Xuecheng; RAO Qian; YANG Tianyi; TIAN Jiayu; XIAO Xiong; GAO Wenjun; LI Wenhong

doi:10.6039/j.issn.1001-0408.2024.13.08

您当前的位置：

首页 >

文章列表页 >

Pharmacokinetic study of the complication of Ephedra sinica and Prunus armeniaca in bronchial asthma model rats

更新时间：2024-07-10

- Pharmacokinetic study of the complication of Ephedra sinica and Prunus armeniaca in bronchial asthma model rats
- ZHONGGUO YAOFANG Vol. 35, Issue 13, Pages: 1588-1593(2024)
- 作者机构：
  
  江西中医药大学药学院，南昌　330004
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.13.08
  CLC： R969.1
- Published：15 July 2024，
  
  Received：31 October 2023，
  
  Revised：23 April 2024，
- 稿件说明：
扫描看全文
陈林林,谢金根,樊学程等.麻黄与杏仁配伍后在支气管哮喘模型大鼠体内的药动学研究 ^Δ[J].中国药房,2024,35(13):1588-1593.

CHEN Linlin,XIE Jingen,FAN Xuecheng,et al.Pharmacokinetic study of the complication of Ephedra sinica and Prunus armeniaca in bronchial asthma model rats[J].ZHONGGUO YAOFANG,2024,35(13):1588-1593.
陈林林,谢金根,樊学程等.麻黄与杏仁配伍后在支气管哮喘模型大鼠体内的药动学研究 ^Δ[J].中国药房,2024,35(13):1588-1593. DOI： 10.6039/j.issn.1001-0408.2024.13.08.

CHEN Linlin,XIE Jingen,FAN Xuecheng,et al.Pharmacokinetic study of the complication of Ephedra sinica and Prunus armeniaca in bronchial asthma model rats[J].ZHONGGUO YAOFANG,2024,35(13):1588-1593. DOI： 10.6039/j.issn.1001-0408.2024.13.08.

摘要

目的

研究麻黄与杏仁配伍后在支气管哮喘模型大鼠血浆和脑脊液中的药动学变化。

方法

将SD雄性大鼠随机分为空白组、模型组、麻黄组（12 g/kg，以生药量计，下同）、杏仁组（6 g/kg）和麻黄-杏仁药对组（麻黄12 g/kg+杏仁6 g/kg），每组6只。除空白组外，其余各组大鼠用2%氯化乙酰胆碱和0.4%磷酸组胺的等体积混合液喷雾诱发支气管哮喘模型，每天1次，连续7 d。每次造模前1 h，各组大鼠灌胃相应药物/生理盐水，每天1次，连续7 d。末次给药并激发哮喘后，分别在不同时间点进行采血和脑脊液收集。将血浆与脑脊液样品预处理后（以葛根素为内标），采用液相色谱-串联质谱法检测两种样品中麻黄碱/伪麻黄碱、甲基麻黄碱和苦杏仁苷的质量浓度。采用DAS 2.0药动学软件通过非房室模型确定主要药动学参数，并比较两药配伍前后药动学参数的变化。

结果

与麻黄组比较，麻黄-杏仁药对组大鼠血浆和脑脊液中麻黄碱/伪麻黄碱、甲基麻黄碱的

max

、AUC

0-21.33 h

（或AUC

0-10.67 h

）均显著减少，CL

、

均显著增加（

＜0.05或

＜0.01）；脑脊液中甲基麻黄碱的

max

显著缩短（

＜0.05）。与杏仁组比较，麻黄-杏仁药对组大鼠血浆中苦杏仁苷的

1/2

显著缩短，CL

显著增加（

＜0.01）；脑脊液中苦杏仁苷的

max

显著缩短，AUC

0-10.67 h

、CL

、

均显著增加（

＜0.01）。

结论

麻黄、杏仁配伍后加快了麻黄生物碱的吸收和消除，从而减少了麻黄生物碱在支气管哮喘模型大鼠体内的累积。

Abstract

OBJECTIVE

To study the pharmacokinetic changes in the plasma and cerebrospinal fluid of bronchial asthma model rats after the complication of

Ephedra sinica

and

Prunus armeniaca

METHODS

SD male rats were randomly divided into blank group， model group，

E. sinica

group （12 g/kg， calculated by raw drug， similarly hereinafter），

P. armeniaca

group （6 g/kg） and

E. sinica

P. armeniaca

drug-pair group （12 g/kg of

E. sinica

+6 g/kg of

P. armeniaca

）， with 6 rats in each group. Except for the blank group， the br

onchial asthma model was induced by spraying rats in each group with an equal volume mixture of 2% acetylcholine chloride and 0.4% histamine phosphate， once a day， for 7 d. One hour before modeling every time， rats in each group were gavaged with the corresponding drug/normal saline， once a day， for 7 d. After the final administration and provocation of asthma， blood and cerebrospinal fluid collection were performed at different time points. The plasma and cerebrospinal fluid samples were pre-treated （with geranylgeranyl as the internal standard）， and the mass concentrations of ephedrine/pseudoephedrine， methyl ephedrine and amygdalin in both samples were determined by liquid chromatography-tandem mass spectrometry. DAS 2.0 pharmacokinetic software was used to determine the main pharmacokinetic parameters through the non-atrial chamber model and to compare the changes of the pharmacokinetic parameters before and after the combination of the two drugs.

RESULTS

Compared with

E. sinica

group，

max

and AUC

0-21.33 h

（or AUC

0-10.67 h

） of ephedrine/pseudoephedrine and methyl ephedrine in the plasma and cerebrospinal fluid of rats were significantly reduced in

E. sinica

P. armeniaca

drug-pair group， while CL

and

were significantly increased （

＜0.05 or

＜0.01）；

max

of methyl ephedrine in the cerebrospinal fluid was significantly shortened （

＜0.05）.Compared with

P. armeniaca

group， the

1/2

of amygdalin in the plasma of rats in

E. sinica

P. armeniaca

drug-pair group was significantly shortened， and CL

was significantly increased （

＜0.01）； the

max

of bitter amygdalin in the cerebrosp

inal fluid was significantly shortened， and the AUC

0-10.67 h

， CL

， and

were significantly increased （

＜0.01）.

CONCLUSIONS

The combination of

E. sinica

and

P. armeniaca

accelerates the absorption and elimination of ephedra alkaloids， thus reducing the accumulation of ephedra alkaloids in the bronchial asthma model rats.

关键词

麻黄杏仁配伍哮喘药动学

Keywords

Prunus armeniacacomplica-tionasthmapharmacokinetics

references

ATHARI S S. Targeting cell signaling in allergic asthma[J]. Signal Transduct Target Ther，2019，4：45.

柏寒，贺梦媛，徐洋，等.中药苦杏仁研究进展及质量标志物的预测分析[J/OL].中华中医药学刊，2023[2024-05-06]. http：//kns.cnki.net/kcms/detail/21.1546.R.20231108. 1639.006.htmlhttp://kns.cnki.net/kcms/detail/21.1546.R.20231108.1639.006.html.

BAI H，HE M Y，XU Y，et al. Research progress of traditional Chinese medicine Armeniacae Semen Amarum and prediction of its Q-markers[J/OL]. Chin Arch Tradit Chin Med，2023[2024-05-06]. http：//kns.cnki.net/kcms/detail/21.1546.R.20231108.1639.006.htmlhttp://kns.cnki.net/kcms/detail/21.1546.R.20231108.1639.006.html.

AL-AZZAM N，ELSALEM L. Leukotriene D4 role in allergic asthma pathogenesis from cellular and therapeutic perspectives[J]. Life Sci，2020，260：118452.

CEREDA C，KUNTZER T. The potential use of ephedrine in Lambert-Eaton myasthenic syndrome：clinical and electrophysiological evaluation[J]. J Neurol，2008，255（8）：1259-1260.

田楠楠，杨茜和，朱雅暄，等. 麻黄的化学成分及其药效作用和药代特征[J]. 中国中药杂志，2022，47（13）：3409-3424.

TIAN N N，YANG X H，ZHU Y X，et al. Mahuang （herbaceous stem of Ephedra spp.）：chemistry，pharmacodynamics，and pharmacokinetics[J]. China J Chin Mater Med，2022，47（13）：3409-3424.

刘小红，杨宗利. 麻黄杏仁不同配伍比例止咳平喘的效果研究[J]. 临床医学研究与实践，2019，4（22）：8-9，12.

LIU X H，YANG Z L. Study on antitussive and antiasthmatic effects of different compatibility ratio of ephedra and almond[J]. Clin Res Pract，2019，4（22）：8-9，12.

杨丽，李莹，王清娴，等. 麻黄-杏仁药对配比与有效成分含量变化规律研究[J]. 中国当代医药，2017，24（5）：4-7，11.

YANG L，LI Y，WANG Q X，et al. Study on the change rule between different compatibility of ephedra-amond drugs and the concentration of effective components[J]. China Mod Med，2017，24（5）：4-7，11.

魏伟，吴希美，李元建. 药理实验方法学[M]. 4版. 北京：人民卫生出版社，2010：71.

WEI W，WU X M，LI Y J. Methodology of pharmacological experiment[M]. 4th edition. Beijing：People’s Health Publishing House，2010：71.

李文宏，雷婷，马嘉鑫，等. 麻杏石甘汤对哮喘模型大鼠气道损伤及EGFR表达的影响[J]. 中国实验方剂学杂志，2022，28（7）：1-10.

LI W H，LEI T，MA J X，et al. Effect of Maxing shigan tang on airway injury and EGFR expression in asthma model rats[J]. Chin J Exp Tradit Med Formulae，2022，28（7）：1-10.

段正昊，杨童雅，吕捷，等. 一种改良的大鼠脑脊液抽取方法[J]. 中国医科大学学报，2019，48（11）：1048-1050.

DUAN Z H，YANG T Y，LYU J，et al. A modified method for extracting cerebrospinal fluid from rats[J]. J China Med Univ，2019，48（11）：1048-1050.

赵晓晓，李亚前，王淑梅，等. 甘露醇对万古霉素在家兔体内血脑屏障透过能力及脑脊液药动学的影响[J]. 中南药学，2020，18（4）：582-586.

ZHAO X X，LI Y Q，WANG S M，et al. Effect of mannitol on blood-brain barrier penetration and pharmacokinetics of cerebrospinal fluid of vancomycin in rabbits[J]. Cent South Pharm，2020，18（4）：582-586.

叶晓滨. 麻黄常用药对化学成分与药理作用的研究进展[J]. 中医研究，2021，34（3）：57-62.

YE X B. Research progress on chemical constituents and pharmacological effects of common Chinese ephedra drugs[J]. Tradit Chin Med Res，2021，34（3）：57-62.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Pharmacokinetic study of small molecule inhibitor SYHA1809 in Beagle dogs

Study on pharmacokinetics of novel lung-targeted Docetaxel liposome in in-situ lung cancer model rabbit

Study on the tissue distribution and pharmacokinetics of PELGE-crebanine nanoparticles in rats

Study on pharmacokinetics of Baihe-zhimu decoction and its single herbs

β-七叶皂苷钠在家兔体内的药动学研究

Related Author

LIU Xiaolin

YANG Hanyu

WANG Xiaoyan

KANG Kai

LIANG Min

WANG Lijuan

LI Rui

CHE Keke

Related Institution

College of Pharmacy， Hebei Medical University

CSPC Zhongqi Pharmaceutical （Shijiazhuang） Co.， Ltd.

School of Pharmacy， Chongqing Medical and Pharmaceutical College/Chongqing Engineering Research Center of Pharmaceutical Sciences

Yaopharma Co.， Ltd.

Dept. of Pharmacy， Chongqing General Hospital

Address：8th Floor, Sihuan Building, No. 129 Daping Main Street, Yuzhong District, Chongqing, China Postal code：400042
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Pharmacokinetic study of the complication of Ephedra sinica and Prunus armeniaca in bronchial asthma model rats

DOI：10.6039/j.issn.1001-0408.2024.13.08

摘要

Abstract

关键词

Keywords

references