Protective effect and mechanism of quercetin on cardiac and renal functions in rats with cardiorenal syndrome

KONG Lujiao; LU Hua; WANG Xin; LI Shuai; LIU Jing; GUO Xiaoyang

doi:10.6039/j.issn.1001-0408.2024.13.12

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Protective effect and mechanism of quercetin on cardiac and renal functions in rats with cardiorenal syndrome

更新时间：2025-09-23

- Protective effect and mechanism of quercetin on cardiac and renal functions in rats with cardiorenal syndrome
- ZHONGGUO YAOFANG Vol. 35, Issue 13, Pages: 1612-1617(2024)
- 作者机构：
  
  邢台市人民医院肾内科，河北邢台　054000
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.13.12
  CLC： R965
- Received：17 January 2024，
  
  Revised：2024-05-19，
  
  Accepted：20 May 2024，
  
  Published：15 July 2024
- 稿件说明：
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孔露娇,鲁华,王心,等.槲皮素对心肾综合征大鼠心肾功能的保护作用及机制 [J].中国药房,2024,35(13):1612-1617.

KONG Lujiao,LU Hua,WANG Xin,et al.Protective effect and mechanism of quercetin on cardiac and renal functions in rats with cardiorenal syndrome[J].ZHONGGUO YAOFANG,2024,35(13):1612-1617.
孔露娇,鲁华,王心,等.槲皮素对心肾综合征大鼠心肾功能的保护作用及机制 [J].中国药房,2024,35(13):1612-1617. DOI： 10.6039/j.issn.1001-0408.2024.13.12.

KONG Lujiao,LU Hua,WANG Xin,et al.Protective effect and mechanism of quercetin on cardiac and renal functions in rats with cardiorenal syndrome[J].ZHONGGUO YAOFANG,2024,35(13):1612-1617. DOI： 10.6039/j.issn.1001-0408.2024.13.12.

摘要

目的

基于磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（Akt）/核因子κB（NF-κB）信号通路探讨槲皮素对心肾综合征（CRS）大鼠心肾功能的保护作用及机制。

方法

取SD大鼠，采用左冠状动脉前降支结扎结合肾急性缺血再灌注的方法构建CRS模型，将造模成功的大鼠随机分为模型组、槲皮素低剂量组（35 mg/kg）、槲皮素高剂量组（70 mg/kg）、槲皮素高剂量+740Y-P组（70 mg/kg槲皮素+3.5 mg/kg PI3K/Akt/NF-κB信号通路激活剂740Y-P），每组12只；另取12只正常大鼠设为假手术组。每天给药1次，连续14 d。给药结束后，检测大鼠心功能指标[左心室射血分数（LVEF）、舒张末期容积（EDV）、等容舒张时间（IVRT）]与肾功能指标[血尿素氮（BUN）、血肌酐（Scr）、24 h尿蛋白]；观察大鼠心肾组织纤维化情况；检测大鼠血清与心肾组织中炎症指标[白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）]水平及心肾组织中PI3K/Akt/NF-κB信号通路相关蛋白表达。

结果

与假手术组比较，模型组大鼠BUN、24 h尿蛋白、Scr水平，心肾组织的胶原容积分数，血清与心肾组织中IL-1β、TNF-α水平，心肾组织中PI3K、Akt、NF-κB p65蛋白磷酸化水平均显著升高（

＜0.05），LVEF、IVRT、EDV均显著降低（

＜0.05）。与模型组比较，槲皮素低、高剂量组大鼠上述指标均显著改善（

＜0.05），且高剂量组的改善效果更好（

＜0.05）。740Y-P逆转了高剂量槲皮素对

各指标的改善作用（

＜0.05）。

结论

槲皮素可减轻CRS大鼠心肾纤维化和功能损伤，其作用机制可能与抑制PI3K/Akt/NF-κB信号通路激活有关。

Abstract

OBJECTIVE

To investigate the protective effect and mechanism of quercetin on the cardiac and renal functions of rats with cardiorenal syndrome （CRS） based on the phosphoinositide 3-kinase （PI3K）/protein kinase B （Akt）/nuclear factor kappa-B （NF-κB） signaling pathway.

METHODS

CRS model of SD rats was induced by left anterior descending coronary artery ligation combined with acute renal ischemia-reperfusion. Model rats were randomly separated into model group， quercetin low-dose group （35 mg/kg）， quercetin high-dose group （70 mg/kg）， high-dose of quercetin+740Y-P group （70 mg/kg quercetin+3.5 mg/kg PI3K/Akt/NF-κB signaling pathway activator 740Y-P）， with 12 rats in each group. Another 12 normal rats were selected as sham operation group. They were given relevant drugs， once a day， for 14 consecutive days. After administration， the cardiac function indexes [left ventricular ejection fraction （LVEF）， end-diastolic volume （EDV）， isovolumic relaxation time （IVRT）] and renal function indicators [blood urea nitrogen （BUN）， 24-hour urine protein， and serum creatinine （Scr）] were detected， and fibrosis in the cardiac and renal tissues was observed； the levels of inflammatory indexes [interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α）] in the serum and cardiac and renal tissues as well as the expression of PI3K/Akt/NF-κB pathway-related proteins in the cardiac and renal tissues were detected.

RESULTS

Compared with sham operation group， the levels of BUN， 24-hour urine protein and Scr， collagen volume fraction of cardiac and renal tissues， the levels of IL-1β and TNF-α in serum and cardiac and renal tissues， and the phosphorylation of PI3K， Akt and NF-κB p65 protein in cardiac and renal tissues were increased significantly in model grou

p （

＜0.05）； the levels of LVEF， IVRT and EDV were reduced significantly （

＜0.05）. Compared with the model group， the above indexes were reversed significantly in quercetin low-dose and high-dose groups （

＜0.05）， and the reversal effect was better in the high-dose group （

＜0.05）. 740Y-P restored the reverse effect of high-dose quercetin on the indexes （

＜0.05）.

CONCLUSIONS

Quercetin can alleviate cardiac and renal fibrosis and function injury， the mechanism of which may be associated with inhibiting the activation of the PI3K/Akt/NF-κB signaling pathway.

关键词

Keywords

references

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