Study on characteristic chromatogram of Yao medicine <italic style="font-style: italic">Kadsura longipedunculata</italic> and its anwulignan content and anti-inflammatory activity

TANG Binglan; GAO Wei; ZHAO Chengjian; OU Chunli; HOU Xiaoli; CHEN Lu; MO Dandan

doi:10.6039/j.issn.1001-0408.2024.14.09

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Study on characteristic chromatogram of Yao medicine Kadsura longipedunculata and its anwulignan content and anti-inflammatory activity

更新时间：2024-07-25

- Study on characteristic chromatogram of Yao medicine Kadsura longipedunculata and its anwulignan content and anti-inflammatory activity
- ZHONGGUO YAOFANG Vol. 35, Issue 14, Pages: 1727-1731(2024)
- 作者机构：
  
  1.广西壮族自治区药用植物园国家中医药传承创新中心/广西药用资源保护与遗传改良重点实验室/广西壮族自治区中药资源智慧创制工程研究中心，南宁　530023
  2.广西壮族自治区药品监督管理局，南宁　530029
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.14.09
  CLC： R917;R285
- Published：30 July 2024，
  
  Received：21 February 2024，
  
  Revised：04 July 2024，
- 稿件说明：
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唐炳兰,高微,赵成坚等.瑶药小钻的特征图谱及其安五脂素含量和抗炎活性研究 ^Δ[J].中国药房,2024,35(14):1727-1731.

TANG Binglan,GAO Wei,ZHAO Chengjian,et al.Study on characteristic chromatogram of Yao medicine Kadsura longipedunculata and its anwulignan content and anti-inflammatory activity[J].ZHONGGUO YAOFANG,2024,35(14):1727-1731.
唐炳兰,高微,赵成坚等.瑶药小钻的特征图谱及其安五脂素含量和抗炎活性研究 ^Δ[J].中国药房,2024,35(14):1727-1731. DOI： 10.6039/j.issn.1001-0408.2024.14.09.

TANG Binglan,GAO Wei,ZHAO Chengjian,et al.Study on characteristic chromatogram of Yao medicine Kadsura longipedunculata and its anwulignan content and anti-inflammatory activity[J].ZHONGGUO YAOFANG,2024,35(14):1727-1731. DOI： 10.6039/j.issn.1001-0408.2024.14.09.

摘要

目的

建立瑶药小钻特征图谱及其主要成分安五脂素含量测定方法，并评价安五脂素抗炎活性。

方法

采用高效液相色谱法，以乙腈-0.5%磷酸溶液为流动相进行梯度洗脱，采用《中药色谱指纹图谱相似度评价系统（2012版）》建立小钻药材的特征图谱和进行相似度评价，并测定小钻中安五脂素含量。以脂多糖诱导的RAW264.7巨噬细胞为炎症细胞模型，考察安五脂素对细胞上清液中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、IL-6水平的影响。

结果

10批小钻样品的特征图谱相似度为0.901～0.994；标定了9个共有峰，指认了3个成分，分别为长南五味子素E、南五味子内酯A、安五脂素；安五脂素的含量为（0.72±0.05）～（1.21±0.03）mg/g（

＝3）。0.125～0.5 μg/mL的安五脂素可显著降低炎症模型细胞上清液中TNF-α、IL-1β、IL-6水平（

＜0.05或

＜0.01）。

结论

建立了小钻药材HPLC特征图谱及其安五脂素含量测定方法；安五脂素可能是小钻药材发挥抗炎作用的有效成分。

Abstract

OBJECTIVE

To establish characteristic chromatogram of Yao medicine

Kadsura longipedunculata

and the method for the content determination of its main component anwulignan， and evaluate the anti-inflammatory activity of anwulignan.

METHODS

HPLC method was performed with acetonitrile-0.5% phosphoric acid solution as the mobile phase for gradient elution. The characteristic chromatogram of

K. longipedunculata

was established and similarity was evaluated by

Similarity Evaluation System for Chromatographic Fingerprint of TCM

（

2012 edition

）. The content of anwulignan in

K. longipedunculata

was determined. Lipopolysaccharide induced RAW264.7 macrophages were selected as inflammatory cell model to investigate the effects of anwulignan on the levels of tumor necrosis factor-α （TNF-α）， interleukin-1β （IL-1β） and IL-6.

RESULTS

The similarities of characteristic chromatogram for 10 batches of

K. longipedunculata

ranged 0.901-0.994， and 9 common peaks were determined； 3 components were identified， such as changnan schisantherin E， kadsulactone A， anwulignan. The contents of anwulignan were （0.72±0.05）-（1.21±0.03） mg/g（

＝3）. Anwulignan of 0.125-0.5 μg/mL greatly decreased the levels of TNF-α， IL-1β and IL-6 in the supernatant of inflammatory model cells （

＜0.05 or

＜0.01）.

CONCLUSIONS

HPLC characteristic chromatogram of

K. longipedunculata

and the method for the content determination of anwulignan are all established， and anwulignan may be the active ingredient of anti-inflammatory effect in

K. longipedunculata

关键词

瑶药小钻HPLC特征图谱质量控制安五脂素抗炎作用

Keywords

HPLCcharacteristic chromatogramquality controlanwulignananti-inflammatory action

references

戴斌. 中国现代瑶药[M]. 南宁：广西科学技术出版社，2009：70-72.

DAI B. Modern Chinese Yao medicine[M]. Nanning：Guangxi Science and Technology Press，2009：70-72.

郭耀杰，郭豪杰，刘久石，等. 长梗南五味子化学成分和药理活性研究进展[J]. 中国现代中药，2015，17（12）：1350-1358.

GUO Y J，GUO H J，LIU J S，et al. Advances in studies on chemical constituents in Kadsura longipedunculata and their pharmacological activities[J]. Mod Chin Med，2015，17（12）：1350-1358.

广西壮族自治区食品药品监督管理局.广西壮族自治区瑶药材质量标准第一卷：2014年版[M].南宁：广西科学技术出版社，2014：155.

Guangxi Zhuang Autonomous Region Food and Drug Administration. Quality standards for Yao medicinal mate-rials in Guangxi Zhuang Autonomous region volume 1：2014 edition [M]. Nanning：Guangxi Science and Techno-logy Press，2014：155.

莫单丹，唐炳兰，周小雷，等. 小钻提取物抗炎镇痛活性研究[J]. 医药导报，2014，33（3）：291-294.

MO D D，TANG B L，ZHOU X L，et al. Anti-inflammation and analgesic effects of the extracts from Kasdsura longipedunculata Finet et Gagnep[J]. Her Med，2014，33（3）：291-294.

覃朗，程娅，莫单丹，等. 瑶药小钻乙酸乙酯提取物抗类风湿关节炎作用及相关机制探讨[J]. 中国药学杂志，2017，52（8）：637-642.

QIN L，CHENG Y，MO D D，et al. Exploration into the anti-rheumatoid arthritis mechanism of ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep[J]. Chin Pharm J，2017，52（8）：637-642.

林慧娇，张馨芸，刘嘉玮，等. 安五脂素对大鼠肠缺血再灌注致肝损伤的保护作用[J]. 食品工业科技，2021，42（7）：330-336.

LIN H J，ZHANG X Y，LIU J W，et al. Effects of anwulignan on the hepatic injury induced by intestinal ischemia-reperfusion in rats[J]. Sci Technol Food Ind，2021，42（7）：330-336.

LIU J W，LIN H J，YUAN L W，et al. Protective effects of anwulignan against HCl/ethanol-induced acute gastric ulcer in mice[J]. Evid Based Complement Alternat Med，2021，2021：9998982.

潘翠柳，莫文坤，潘真真，等. 红鱼眼不同提取物含药血清对脂多糖诱导RAW264.7细胞炎症的影响[J]. 广西中医药大学学报，2023，26（6）：46-50.

PAN C L，MO W K，PAN Z Z，et al. Drug serum from different extracts of Phyllanthus reticulatus Poir. on inflammatory factors in LPS-induced macrophage RAW264.7[J]. J Guangxi Univ Chin Med，2023，26（6）：46-50.

颜丽珊，邱新宇，康建英，等. 天山雪莲总酚酸对脂多糖刺激的RAW264.7细胞炎症因子释放的影响及其机制研究[J]. 中国现代中药，2023，25（10）：2109-2118.

YAN L S，QIU X Y，KANG J Y，et al. Study on effects of total phenolic acids from Saussureae involucratae herba on release of inflammatory mediators in lipopolysaccharide-stimulated RAW264.7 macrophages and its mechanism[J]. Mod Chin Med，2023，25（10）：2109-2118.

覃朗，莫单丹，程娅，等. 小钻木脂素类化合物戈米辛R的体外抗炎机制研究[J]. 中国药学杂志，2022，57（18）：1543-1548.

QIN L，MO D D，CHENG Y，et al. Research on in vitro anti-inflammatory mechanism of gomisin R，the lignans of Kadsura longipedunculata Finet et Gagnep[J]. Chin Pharm J，2022，57（18）：1543-1548.

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Study on characteristic chromatogram of Yao medicine Kadsura longipedunculata and its anwulignan content and anti-inflammatory activity

DOI：10.6039/j.issn.1001-0408.2024.14.09

摘要

Abstract

关键词

Keywords

references