Mechanism of tetrahydropalmatine inhibiting oxidative stress damage of VSMCs induced by PDGF-BB

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Mechanism of tetrahydropalmatine inhibiting oxidative stress damage of VSMCs induced by PDGF-BB

更新时间：2024-08-11

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- Mechanism of tetrahydropalmatine inhibiting oxidative stress damage of VSMCs induced by PDGF-BB
- ZHONGGUO YAOFANG Vol. 35, Issue 15, Pages: 1855-1861(2024)
- 作者机构：
  
  遵义医科大学附属医院心血管内科，贵州遵义　563000
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.15.10
  CLC： R965;R543
- Published：15 August 2024，
  
  Received：21 March 2024，
  
  Revised：05 June 2024，
- 稿件说明：
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陈文明,蹇明辉.延胡索乙素改善PDGF-BB诱导的VSMCs氧化应激损伤的机制 ^Δ[J].中国药房,2024,35(15):1855-1861.

CHEN Wenming,JIAN Minghui.Mechanism of tetrahydropalmatine inhibiting oxidative stress damage of VSMCs induced by PDGF-BB[J].ZHONGGUO YAOFANG,2024,35(15):1855-1861.
陈文明,蹇明辉.延胡索乙素改善PDGF-BB诱导的VSMCs氧化应激损伤的机制 ^Δ[J].中国药房,2024,35(15):1855-1861. DOI： 10.6039/j.issn.1001-0408.2024.15.10.

CHEN Wenming,JIAN Minghui.Mechanism of tetrahydropalmatine inhibiting oxidative stress damage of VSMCs induced by PDGF-BB[J].ZHONGGUO YAOFANG,2024,35(15):1855-1861. DOI： 10.6039/j.issn.1001-0408.2024.15.10.

摘要

目的

2

研究延胡索乙素（Thp）对血小板衍生生长因子-BB（PDGF-BB）诱导的大鼠主动脉血管平滑肌细胞（VSMCs）氧化应激损伤的保护作用，并基于核因子红系2相关因子2（Nrf2）/血红素加氧酶1（HO-1）信号通路探究其可能机制。

方法

2

在Thp抑制PDGF-BB诱导的VSMCs氧化应激损伤效应研究中，将VSMCs分为对照组、PDGF-BB组（25 ng/mL）及Thp低、中、高浓度组（5、10、20 mg/mL）。在Thp作用机制研究（沉默Nrf2）中，将VSMCs分为PDGF-BB+阴性对照siRNA（NC-siNrf2）组（25 ng/mL PDGF-BB+NC-siNrf2），PDGF-BB+Thp+NC-siNrf2组（25 ng/mL PDGF-BB+10 mg/mL Thp+NC-siNrf2），PDGF-BB+Nrf2小干扰RNA（siNrf2）组（25 ng/mL PDGF-BB+siNrf2），PDGF-BB+Thp+siNrf2组（25 ng/mL PDGF-BB+10.0 mg/mL Thp+siNrf2）。2个实验均检测VSMCs的增殖、迁移能力，活性氧（ROS）水平，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及Nrf2和HO-1蛋白表达。

结果

2

与对照组比较，PDGF-BB组VSMCs的增殖、迁移能力显著增强（

P

＜0.01），ROS水平显著升高（

P

＜0.01），SOD、CAT活性及Nrf2、HO-1蛋白的相对表达量均显著降低（

P

＜0.01）；与PDGF-BB组比较，Thp不同浓度组VSMCs的增殖、迁移能力均显著下降（

P

＜0.01），ROS水平均显著降低（

P

＜0.01），SOD、CAT活性及Nrf2、HO-1蛋白的相对表达量均显著升高（

P

＜0.01）。沉默Nrf2可显著逆转Thp对PDGF-BB诱导VSMCs氧化应激损伤的改善作用（

P

＜0.01）。

结论

2

Thp可以通过激活Nrf2介导的抗氧化防御途径来降低VSMCs的氧化应激水平，从而抑制VSMCs的增殖、迁移。

Abstract

OBJECTIVE

2

To study the protective effect of tetrahydropalmatine （Thp） on platelet-derived growth factor-BB （PDGF-BB） induced oxidative stress injury in vascular smooth muscle cells （VSMCs） of rats， and to explore its possible mechanism based on the nuclear factor-erythroid 2-related factor 2 （Nrf2）/heme oxygenase （HO-1） signaling pathway.

METHODS

2

In the study about Thp inhibiting PDGF-BB-induced oxidative stress injury in VSMCs， VSMCs were divided into control group， PDGF-BB group （25 ng/mL）， and Thp low-concentration， medium-concentration and high-concentration groups （5， 10， 20 mg/mL）. In the Thp mechanism experiment （silencing Nrf2）， VSMCs were divided into PDGF-BB+negative control of siRNA （NC-siNrf2） group （25 ng/mL PDGF-BB+NC-siNrf2）， PDGF-BB+Thp+NC-siNrf2 group （25 ng/mL PDGF-BB+10 mg/mL Thp+NC-siNrf2）， PDGF-BB+Nrf2 small interfering RNA （siNrf2） group （25 ng/mL PDGF-BB+siNrf2） and PDGF-BB+Thp+siNrf2 group （25 ng/mL PDGF-BB+10.0 mg/mL Thp+siNrf2）. The proliferative and migratory capabilities of VSMCs， the level of reactive oxygen species （ROS）， the activities of superoxide dismutase （SOD） and catalase （CAT） as well as the protein expressions of Nrf2 and HO-1 in VSMCs were all detected in two experiments.

RESULTS

2

Compared with the control group， the proliferative and migratory capabilities of VSMCs in the PDGF-BB group were significantly enhanced （

P

＜0.01）， and the level of ROS significantly increased （

P

＜0.01）， while the activities of SOD and CAT， and the relative expressions of Nrf2 and HO-1 protein significantly decreased （

P

＜0.01）. Compared with the PDGF-BB group， the proliferative and migratory capabilities of VSMCs in the Thp groups at different concentrations were significantly reduced （

P

＜0.01）， the levels of ROS were significantly reduced， while the activities of SOD and CAT， and relative expressions of Nrf2 and HO-1 were significantly enhanced （

P

＜0.01）. Silencing Nrf2 significantly reversed the improvement of Thp on the oxidative stress damage of VSMCs induced by PDGF-BB （

P

＜0.01）.

CONCLUSIONS

2

Thp can reduce the oxidative stress level of VSMCs by activating the Nrf2-mediated antioxidant defense pathway， thereby inhibiting the proliferation and migration of VSMCs.

关键词

延胡索乙素血小板衍生生长因子-BB血管平滑肌细胞Nrf2/HO-1信号通路氧化应激

Keywords

platelet-derived growth factor-BBvascular smooth muscle cellsNrf2/HO-1 signaling pathwayoxidative stress

references

REMPAKOS A，KOSTANTINIS S，SIMSEK B，et al. Update on chronic total occlusion percutaneous coronary intervention[J]. J Invasive Cardiol，2023，35（4）：E194-E204.

TAO S Y，TANG X W，YU L T，et al. Prognosis of coronary heart disease after percutaneous coronary intervention：a bibliometric analysis over the period 2004-2022[J]. Eur J Med Res，2023，28（1）：311.

PELLICCIA F，ZIMARINO M，NICCOLI G，et al. In-stent restenosis after percutaneous coronary intervention：emerging knowledge on biological pathways[J]. Eur Heart J Open，2023，3（5）：oead083.

DU Q Y，MENG X L，WANG S H. A comprehensive review on the chemical properties，plant sources，pharmacological activities，pharmacokinetic and toxicological cha- racteristics of tetrahydropalmatine[J]. Front Pharmacol，2022，13：890078.

蹇明辉，陈文明，张怡. 延胡索乙素对高糖诱导H9c2细胞凋亡和氧化应激的抑制作用[J]. 遵义医科大学学报，2022，45（3）：317-321.

JIAN M H，CHEN W M，ZHANG Y. Inhibitory effect of tetrahydropalmatine on apoptosis and oxidative stress of H9c2 cells induced by high glucose[J]. J Zunyi Med Univ，2022，45（3）：317-321.

MCCORD J M，GAO B F，HYBERTSON B M. The complex genetic and epigenetic regulation of the Nrf2 pathways：a review[J]. Antioxidants （Basel），2023，12（2）：366.

SI J Y，MENG R R，GAO P，et al. Linagliptin protects rat carotid artery from balloon injury and activates the Nrf2 antioxidant pathway[J]. Exp Anim，2019，68（1）：81-90.

仇治梅，陈文明，王艳，等. 人参皂苷Rg3对大鼠血管平滑肌细胞凋亡及凋亡相关基因表达的影响[J]. 遵义医科大学学报，2020，43（4）：419-424.

QIU Z M，CHEN W M，WANG Y，et al. Effect of ginseno-side Rg3 on apoptosis and expression of apoptosis related genes of vascular smooth muscle cells in rats[J]. J Zunyi Med Univ，2020，43（4）：419-424.

李岩，安芸，张赫彬，等. 华良姜素对PDGF-BB诱导血管平滑肌细胞增殖和迁移的影响[J]. 中国动脉硬化杂志，2023，31（10）：865-871.

LI Y，AN Y，ZHANG H B，et al. Effects of jaranol on proliferation and migration of vascular smooth muscle cells induced by PDGF-BB[J]. Chin J Arterioscler，2023，31（10）：865-871.

YIN X Z，LI W B，ZHANG J X，et al. AMPK-mediated metabolic switching is high effective for phytochemical levo-tetrahydropalmatine （l-THP） to reduce hepatocellular carcinoma tumor growth[J]. Metabolites，2021，11（12）：811.

TANG H Y，CHEN A Q，ZHANG H，et al. Vascular smooth muscle cells phenotypic switching in cardiovascular diseases[J]. Cells，2022，11（24）：4060.

QIU L Q，HU L L，LIU X X，et al. Physalin B inhibits PDGF-BB-induced VSMC proliferation，migration and phenotypic transformation by activating the Nrf2 pathway[J]. Food Funct，2021，12（21）：10950-10966.

KIM N，HWANGBO C，LEE S，et al. Eupatolide inhibits PDGF-induced proliferation and migration of aortic smooth muscle cells through ROS-dependent heme oxygenase-1 induction[J]. Phytother Res，2013，27（11）：1700-1707.

ZHANG J，CAI W Y，FAN Z X，et al. MicroRNA-24 inhibits the oxidative stress induced by vascular injury by activating the Nrf2/HO-1 signaling pathway[J]. Atherosclerosis，2019，290：9-18.

ZHANG C C，KONG X H，MA D L.MiR-141-3p inhibits vascular smooth muscle cell proliferation and migration via regulating Keap1/Nrf2/HO-1 pathway[J]. IUBMB Life，2020，72（10）：2167-2179.

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