Regulation effects and mechanism of liquiritin on immune function in gastric cancer-bearing mice

LAN Chunyan; YANG Xiaolan; HE Xuefeng; ZHAO Dan; YANG Haiyan

doi:10.6039/j.issn.1001-0408.2024.15.11

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Regulation effects and mechanism of liquiritin on immune function in gastric cancer-bearing mice

更新时间：2025-09-23

- Regulation effects and mechanism of liquiritin on immune function in gastric cancer-bearing mice
- ZHONGGUO YAOFANG Vol. 35, Issue 15, Pages: 1862-1867(2024)
- 作者机构：
  
  1.达州中医药职业学院药学院，四川达州　635000
  2.成都中医药大学附属医院针灸学校中药教研室，成都　610000
  3.达州市中西医结合医院临床药学科，四川达州　635000
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.15.11
  CLC： R965
- Received：22 January 2024，
  
  Revised：2024-06-07，
  
  Accepted：17 June 2024，
  
  Published：15 August 2024
- 稿件说明：
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兰春燕,杨小兰,贺雪峰,等.甘草苷对胃癌荷瘤小鼠免疫功能的调节作用及机制研究 [J].中国药房,2024,35(15):1862-1867.

LAN Chunyan,YANG Xiaolan,HE Xuefeng,et al.Regulation effects and mechanism of liquiritin on immune function in gastric cancer-bearing mice[J].ZHONGGUO YAOFANG,2024,35(15):1862-1867.
兰春燕,杨小兰,贺雪峰,等.甘草苷对胃癌荷瘤小鼠免疫功能的调节作用及机制研究 [J].中国药房,2024,35(15):1862-1867. DOI： 10.6039/j.issn.1001-0408.2024.15.11.

LAN Chunyan,YANG Xiaolan,HE Xuefeng,et al.Regulation effects and mechanism of liquiritin on immune function in gastric cancer-bearing mice[J].ZHONGGUO YAOFANG,2024,35(15):1862-1867. DOI： 10.6039/j.issn.1001-0408.2024.15.11.

摘要

目的

基于Janus激酶2（JAK2）/信号转导及转录激活蛋白3（STAT3）信号通路研究甘草苷（LIQ）对胃癌荷瘤小鼠免疫功能的调节作用及机制。

方法

皮下注射胃癌细胞MFC建立小鼠胃癌荷瘤模型，将造模成功的小鼠分为模型组（Model组）、低剂量LIQ组（LIQ-L组，20 mg/kg）、高剂量LIQ组（LIQ-H组，40 mg/kg）、高剂量LIQ+JAK2激活剂香豆霉素A1组（LIQ-H+香豆霉素A1组，40 mg/kg LIQ+1 mg/kg香豆霉素A1），每组12只；另取12只不造模小鼠设为正常组（Normal组）。各组小鼠灌胃/腹腔注射相应药物或生理盐水，每日给药1次，连续14 d。末次给药后，测定小鼠胃癌肿瘤的体积、质量以及脏器指数；检测外周血中CD4

、CD8

T淋巴细胞百分率；观察胃癌肿瘤组织病理形态学变化；检测胃癌肿瘤组织中JAK2/STAT3信号通路相关蛋白和白细胞介素6（IL-6）蛋白表达水平。

结果

与Normal组相比，Model组小鼠胸腺指数、脾脏指数、外周血中CD8

T淋巴细胞百分率均显著升高（

＜0.05），外周血中CD4

T淋巴细胞百分率显著降低（

＜0.05）。与Model组相比，LIQ-L组、LIQ-H组小鼠上述指标均显著逆转（

＜0.05），胃癌肿瘤的体积、质量以及肿瘤组织中JAK2、STAT3蛋白的磷酸化水平和IL-6蛋白的表达水平均显著降低（

＜0.05），且LIQ的作用具有剂量依赖性（

＜0.05）；肿瘤组织细胞出现不同程度的排列疏松、空泡化、分布不均匀等情况。加入JAK2激活剂香豆霉素A1减弱了LIQ对胃癌荷瘤小鼠免疫功能的改善作用和对胃癌肿瘤的抑制作用（

＜0.05）。

结论

LIQ可通过抑制JAK2/STAT3信号通路，改善胃癌荷瘤小鼠的免疫功能，从而发挥抗肿瘤作用。

Abstract

OBJECTIVE

To study the regulation effects and mechanism of liquiritin （LIQ） on immune function in gastric cancer-bearing mice based on the Janus kinase 2 （JAK2）/signal transducer and activator of transcription 3 （STAT3） pathway.

METHODS

Gastric cancer cells MFC were injected subcutaneously to establish gastric cancer-bearing model of mice. The model mice were divided into model group， LIQ low-dose group （LIQ-L group， 20 mg/kg）， LIQ high-dose group （LIQ-H group， 40 mg/kg）， and high-dose LIQ+JAK2 activator coumermycin A1 group （LIQ-H+coumermycin A1 group， 40 mg/kg LIQ+1 mg/kg coumermycin A1）， with 12 mice in each group. Another 12 mice without modeling were set as normal group. Mice in each group were given the corresponding drug or normal saline by intragastric administration/intraperitoneal injection， once a day， for consecutive 14 days. After the last administration， the volume and mass of gastric cancer tumor and organ index were measured. The percentages of CD4

and CD8

T lymphocytes were detected in peripheral blood. The histopathological morphology of gastric cancer tumor tissues was observed， and the expression levels of JAK2/STAT3 signaling pathway-related proteins and interleukin-6 （IL-6） protein in gastric cancer tumor tissues were detected.

RESULTS

Compared with the normal group， the thymus index， spleen index， and the percentage of CD8

T lymphocyte in the peripheral blood of mice were obviously increased in model group （

＜0.05）， while the percentage of CD4

T lymphocyte in the peripheral blood were decreased （

＜0.05）. Compared with model group， the above indexes in LIQ-L group and LIQ-H group were significantly reversed （

＜0.05）， while the volume and mass of gastric cancer tumor， the phosphorylation levels of JAK2 and STAT3 protein and the expression level of IL-6 protein were significantly decreased in tumor tissue （

＜0.05）， and the effect of LIQ was in a dose-dependent manner （

＜0.05）； the tumor cells showed varying degrees of loose arrangement， vacuolization， and uneven distribution. JAK2 activator coumermycin A1 weakened the improvement effect of LIQ on the immune function of gastric cancer-bearing mice and its inhibitory effect on gastric cancer tumors （

＜0.05）.

CONCLUSIONS

LIQ can improve the immune function of gastric cancer-bearing mice by inhibiting the activation of JAK2/STAT3 signaling pathway， thus playing an anti-tumor role.

关键词

Keywords

references

BRAY F ， FERLAY J ， SOERJOMATARAM I ， et al . Global cancer statistics 2018：GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J ] . CA Cancer J Clin ， 2018 ， 68 （ 6 ）： 394 - 424 .

MACHLOWSKA J ， BAJ J ， SITARZ M ， et al . Gastric cancer：epidemiology，risk factors，classification，genomic characteristics and treatment strategies [J ] . Int J Mol Sci ， 2020 ， 21 （ 11 ）： 4012 .

GULLO I ， GRILLO F ， MASTRACCI L ， et al . Precan- cerous lesions of the stomach，gastric cancer and hereditary gastric cancer syndromes [J ] . Pathologica ， 2020 ， 112 （ 3 ）： 166 - 185 .

ABBOTT M ， USTOYEV Y . Cancer and the immune system：the history and background of immunotherapy [J ] . Semin Oncol Nurs ， 2019 ， 35 （ 5 ）： 150923 .

WANG S M ， LONG S Q ， DENG Z Y ， et al . Positive role of Chinese herbal medicine in cancer immune regulation [J ] . Am J Chin Med ， 2020 ， 48 （ 7 ）： 1577 - 1592 .

QIN J Y ， CHEN J R ， PENG F ， et al . Pharmacological activities and pharmacokinetics of liquiritin：a review [J ] . J Ethnopharmacol ， 2022 ， 293 ： 115257 .

王曼，何元庆，李敏，等 . 玉屏风和甘草提取物对白羽肉鸡生长性能和免疫功能的影响 [J ] . 饲料工业， 2022 ， 43 （ 9 ）： 15 - 19 .

WANG M ， HE Y Q ， LI M ， et al . Effect of dietary supplementation of Yupingfeng and licorice extract on the growth performance and immune function in white feather broiler chickens [J ] . Feed Ind ， 2022 ， 43 （ 9 ）： 15 - 19 .

LIU B ， HUANG J ， XIAO J ， et al . The Streptococcus virulence protein PepO triggers anti-tumor immune responses by reprograming tumor-associated ma crophages in a mouse triple negative breast cancer model [J ] . Cell Biosci ， 2023 ， 13 （ 1 ）： 198 .

刘建波，李白羽，柯善保，等 . 虫草素抑制JAK2/STAT3通路减轻肺癌大鼠放射性免疫功能损伤 [J ] . 肿瘤防治研究， 2021 ， 48 （ 11 ）： 994 - 998 .

LIU J B ， LI B Y ， KE S B ， et al . Cordycepin attenuates immune function injury in a rat model of lung cancer after radiotherapy by inhibiting JAK2/STAT3 pathway [J ] . Cancer Res Prev Treat ， 2021 ， 48 （ 11 ）： 994 - 998 .

李智鹏，林鑫，李良庆，等 . AG490抑制M2样巨噬细胞JAK2/STAT3通路影响胃癌细胞增殖和凋亡 [J ] . 西安交通大学学报（医学版）， 2023 ， 44 （ 5 ）： 709 - 716 .

LI Z P ， LIN X ， LI L Q ， et al . AG490 regulates JAK2/STAT3 pathway in M2-like macrophages via promoting the proliferation of gastric cancer cells and inhibiting apoptosis [J ] . J Xi’an Jiaotong Univ Med Sci ， 2023 ， 44 （ 5 ）： 709 - 716 .

熊伟，韩华，陈华敏，等 . 柠檬苦素对MFC胃癌荷瘤模型小鼠免疫功能及凋亡相关因子表达的影响 [J ] . 中国药房， 2021 ， 32 （ 7 ）： 845 - 850 .

XIONG W ， HAN H ， CHEN H M ， et al . Effects of limonin on immune function and apoptosis-related factors expres- sion in MFC gastric cancer bearing model mice [J ] . China Pharm ， 2021 ， 32 （ 7 ）： 845 - 850 .

邓溪川，刘璐，王占利，等 . 甘草苷调控PI3K/Akt/m-TOR信号通路对口腔鳞状细胞癌细胞及裸鼠移植瘤小鼠的作用研究 [J ] . 实用口腔医学杂志， 2021 ， 37 （ 1 ）： 10 - 14 .

DENG X C ， LIU L ， WANG Z L ， et al . The effects of liquiritin on PI3K/Akt/m-TOR signaling pathway in oral squamous cell carcinoma cells and in tumor bearing nude mice [J ] . J Pract Stomatol ， 2021 ， 37 （ 1 ）： 10 - 14 .

鲁慧东，李艳梅 . 白术内酯Ⅲ通过调节JAK2/STAT3信号通路减轻溃疡性结肠炎模型小鼠肠道损伤 [J ] . 中国病理生理杂志， 2023 ， 39 （ 1 ）： 142 - 149 .

LU H D ， LI Y M . Atractylenolide Ⅲ attenuates intestinal damage in ulcerative colitis model mice by regulating JAK2/STAT3 signaling pathway [J ] . Chin J Pathophysiol ， 2023 ， 39 （ 1 ）： 142 - 149 .

曲笑锋，石菊，李铁锡，等 . 复方万年青胶囊联合氟尿嘧啶注射液（5-FU）对胃癌荷瘤小鼠的免疫调节作用及其抗癌机制研究 [J ] . 人参研究， 2022 ， 34 （ 1 ）： 28 - 31 .

QU X F ， SHI J ， LI T X ， et al . Immunomodulatory effect and anticancer mechanism of Fufang wannianqing capsule combined with fluorouracil injection（5-FU） on gastric cancer bearing mice [J ] . Ginseng Res ， 2022 ， 34 （ 1 ）： 28 - 31 .

NI H D ， XU M ， XIE K Y ， et al . Liquiritin alleviates pain through inhibiting CXCL1/CXCR2 signaling pathway in bone cancer pain rat [J ] . Front Pharmacol ， 2020 ， 11 ： 436 .

WANG J R ， LI T Z ， WANG C ， et al . Liquiritin inhibits proliferation and induces apoptosis in HepG2 hepatocellular carcinoma cells via the ROS-mediated MAPK/AKT/NF-κB signaling pathway [J ] . Naunyn Schmiedebergs Arch Pharmacol ， 2020 ， 393 （ 10 ）： 1987 - 1999 .

JIN Y ， KANG Y L ， WANG M H ， et al . Targeting polari- zed phenotype of microglia via IL6/JAK2/STAT3 signa- ling to reduce NSCLC brain metastasis [J ] . Signal Transduct Target Ther ， 2022 ， 7 （ 1 ）： 52 .

XIAO Y Y ， LI Y ， SHI D N ， et al . MEX3C-mediated decay of SOCS3 mRNA promotes JAK2/STAT3 signaling to facilitate metastasis in hepatocellular carcinoma [J ] . Cancer Res ， 2022 ， 82 （ 22 ）： 4191 - 4205 .

刘贵廷，杨柳 . SOX9基因下调JAK2/STAT3信号诱导肺癌细胞凋亡及降低免疫逃逸相关因子表达的研究 [J ] . 中国免疫学杂志， 2019 ， 35 （ 1 ）： 59 - 63 .

LIU G T ， YANG L . Down-regulation of JAK2/STAT3 signal by SOX9 gene to induce apoptosis and decrease expression of immune escape related factors in lung cancer cells [J ] . Chin J Immunol ， 2019 ， 35 （ 1 ）： 59 - 63 .

张伟，于鸣，李伟，等 . 白皮杉醇调节JAK2/STAT3信号通路对胆囊癌细胞恶性生物学行为的影响 [J ] . 河北医药， 2023 ， 45 （ 14 ）： 2090 - 2094 .

ZHANG W ， YU M ， LI W ， et al . Influence of piceatannol on malignant biological behavior of gallbladder cancer cells by regulating JAK2/STAT3 signaling pathway [J ] . Hebei Med J ， 2023 ， 45 （ 14 ）： 2090 - 2094 .

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