Effect mechanism of Gualou guizhi granule on neurological function and synaptic plasticity in rats with cerebral ischemia-reperfusion injury

CHEN Wenting; CHEN Shiyi; LI Yanan; FENG Yi; LUO Shuping; ZHANG Yuqin

doi:10.6039/j.issn.1001-0408.2024.16.03

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Effect mechanism of Gualou guizhi granule on neurological function and synaptic plasticity in rats with cerebral ischemia-reperfusion injury

更新时间：2024-08-23

- Effect mechanism of Gualou guizhi granule on neurological function and synaptic plasticity in rats with cerebral ischemia-reperfusion injury
- ZHONGGUO YAOFANG Vol. 35, Issue 16, Pages: 1951-1956(2024)
- 作者机构：
  
  福建中医药大学药学院，福州　350122
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.16.03
  CLC： R965;R285.5
- Published：30 August 2024，
  
  Received：03 April 2024，
  
  Revised：24 July 2024，
- 稿件说明：
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陈雯婷,陈诗怡,李亚男等.栝楼桂枝颗粒对脑缺血再灌注损伤大鼠神经功能及突触可塑性的影响机制 ^Δ[J].中国药房,2024,35(16):1951-1956.

CHEN Wenting,CHEN Shiyi,LI Yanan,et al.Effect mechanism of Gualou guizhi granule on neurological function and synaptic plasticity in rats with cerebral ischemia-reperfusion injury[J].ZHONGGUO YAOFANG,2024,35(16):1951-1956.
陈雯婷,陈诗怡,李亚男等.栝楼桂枝颗粒对脑缺血再灌注损伤大鼠神经功能及突触可塑性的影响机制 ^Δ[J].中国药房,2024,35(16):1951-1956. DOI： 10.6039/j.issn.1001-0408.2024.16.03.

CHEN Wenting,CHEN Shiyi,LI Yanan,et al.Effect mechanism of Gualou guizhi granule on neurological function and synaptic plasticity in rats with cerebral ischemia-reperfusion injury[J].ZHONGGUO YAOFANG,2024,35(16):1951-1956. DOI： 10.6039/j.issn.1001-0408.2024.16.03.

摘要

目的

研究栝楼桂枝颗粒对脑缺血再灌注损伤大鼠神经功能和突触可塑性的影响机制。

方法

大鼠随机分为假手术组、模型组、栝楼桂枝颗粒组（3.6 g/kg），每组6只。采用线栓法构建大鼠脑缺血再灌注损伤模型，造模后2 h灌胃相应药物或生理盐水，每日1次，连续7 d。末次给药后，评价大鼠神经功能[以改良神经功能缺损评分（mNSS）和转角百分率计]，检测大鼠缺血侧大脑皮层神经元凋亡率，生长相关蛋白43（GAP43）、微管相关蛋白2（MAP2）阳性表达情况，神经元核抗原（NeuN）、神经突触素1（Syn-1）、突触后致密蛋白95（PSD-95）、过氧化物酶体增殖物激活受体γ（PPARγ）、脑源性神经营养因子（BDNF）、原肌球蛋白激酶受体B（TrkB）蛋白以及NeuN、Syn-1、PSD-95 mRNA表达情况。

结果

与模型组比较，栝楼桂枝颗粒组大鼠mNSS、转角百分率、神经元凋亡率均显著降低（

＜0.01）；GAP43呈弱免疫反应性，MAP2呈中等免疫反应性；NeuN、Syn-1、PSD-95、PPARγ、BDNF、TrkB蛋白以及Syn-1、NeuN、PSD-95 mRNA表达量均显著升高（

＜0.05或

＜0.01）。

结论

栝楼桂枝颗粒可通过激活BDNF/TrkB信号通路来提高突触可塑性，从而减轻脑缺血再灌注损伤大鼠的神经功能损伤。

Abstract

OBJECTIVE

To investigate the effect mechanism of Gualou guizhi granule on neurological function and synaptic plasticity in rats with cerebral ischemia-reperfusion injury.

METHODS

The rats were randomly divided into sham operation group， model group and Gualou guizhi granule group （3.6 g/kg）， with 6 rats in each group. The cerebral ischemia-reperfusion injury model was established by the suture occlusion method. Two hours after modeling， the model rats were given relevant medicine or normal saline intragastrically， once a day， for 7 consecutive days. After the last medication， the neurological function of rats was evaluated [calculated by modified neurological severity scores （mNSS） and corner turning percentage]； the neuronal apoptosis rate of brain histiocyte in the ischemic side of rats was detected in each group； the positive expressions of growth associated protein 43 （GAP43） and microtubule associated protein 2 （MAP2） were detected； protein expressions of neuron-specific nuclear protein （NeuN）， synaptophysin-1 （Syn-1）， postsynaptic density protein-95 （PSD-95）， peroxisome proliferators-activated receptor γ （PPARγ）， brain-derived neurotrophic factor （BDNF） and tropomyosin receptor kinase B （TrkB）， as well as mRNA expressions of NeuN， Syn-1 and PSD-95 were detected.

RESULTS

Compared with the model group， mNSS， corner turning percentage and neuronal apoptotic rate were decreased significantly in Gualou guizhi granule group （

＜0.01）； GAP43 represented weak immunoreactivity， and MAP2 represented moderate immunoreactivity； protein expressions of NeuN， Syn-1， PSD-95， PPARγ， BDNF， TrkB and mRNA expressions of Syn-1， NeuN， PSD-95 were all increased significantly （

＜0.05 or

＜0.01）.

CONCLUSIONS

Gualou guizhi granule can promote synaptic plasticity by activating BDNF/TrkB signaling pathway， thus playing a protective role in cerebral ischemia-reperfusion injury in rats.

关键词

栝楼桂枝颗粒脑缺血再灌注损伤突触可塑性脑源性神经营养因子

Keywords

cerebral ischemia-reperfusion injurysynaptic plasticityBDNF

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