浏览全部资源
扫码关注微信
- Journal Information
- Archive
- Submission
- For Reviewers
- Statement and Policies
Effects of
Codonopsis pilosula polysaccharide on gastric mucosal injury in rats with chronic atrophic gastritis- ZHONGGUO YAOFANG Vol. 35, Issue 16, Pages: 1985-1990(2024)
- 作者机构：
1.河南中医药大学第一附属医院脾胃肝胆科，郑州 450003
2.河南中医药大学第一附属医院消化内镜中心，郑州 450003
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2024.16.08
CLC： R285.5;R573.3;R965Published：30 August 2024，
Received：02 February 2024，
Revised：12 June 2024，
- 稿件说明：
扫 描 看 全 文
张然,杨坤,曾震军等.党参多糖对慢性萎缩性胃炎大鼠胃黏膜损伤的影响 Δ[J].中国药房,2024,35(16):1985-1990.
ZHANG Ran,YANG Kun,ZENG Zhenjun,et al.Effects of Codonopsis pilosula polysaccharide on gastric mucosal injury in rats with chronic atrophic gastritis[J].ZHONGGUO YAOFANG,2024,35(16):1985-1990.
张然,杨坤,曾震军等.党参多糖对慢性萎缩性胃炎大鼠胃黏膜损伤的影响 Δ[J].中国药房,2024,35(16):1985-1990. DOI： 10.6039/j.issn.1001-0408.2024.16.08.
ZHANG Ran,YANG Kun,ZENG Zhenjun,et al.Effects of Codonopsis pilosula polysaccharide on gastric mucosal injury in rats with chronic atrophic gastritis[J].ZHONGGUO YAOFANG,2024,35(16):1985-1990. DOI： 10.6039/j.issn.1001-0408.2024.16.08.
摘要
目的
2
探究党参多糖（CPP）调节核转录因子红系2相关因子2（Nrf2）/血红素加氧酶1（HO-1）信号通路对慢性萎缩性胃炎（CAG）大鼠胃黏膜损伤的影响。
方法
2
将大鼠随机分为对照组，模型组，CPP低、中、高剂量组（CPP 10、20、40 mg/kg）及ML385组（Nrf2抑制剂ML385 30 mg/kg联用CPP 40 mg/kg），每组10只，采用
N
-甲基-
N
′-硝基-
N
-亚硝基胍联合不规律饮食法建立CAG大鼠模型后，连续给药6周。HE染色观察胃黏膜组织病理形态变化；检测血清胃泌素（GAS）、胃动素（MTL）、胃蛋白酶（PP）及胃黏膜组织肿瘤坏死因子α（TNF-α）、白细胞介素8（IL-8）、丙二醛（MDA）、超氧化物歧化酶（SOD）水平；TUNEL染色观察胃黏膜组织细胞凋亡情况；免疫组化法观察胃黏膜组织Nrf2、B淋巴细胞瘤-2（Bcl-2）相关X蛋白（Bax）表达；Western blot法检测胃黏膜组织Nrf2、HO-1、Bax、Bcl-2蛋白表达。
结果
2
与对照组比较，模型组大鼠胃黏膜组织损伤；GAS、MTL、PP、SOD水平，Nrf2、HO-1、Bcl-2蛋白表达水平均显著降低（
P
＜0.05）；MDA、TNF-α、IL-8水平，细胞凋亡指数，Bax蛋
白表达水平均显著升高（
P
＜0.05）。与模型组比较，CPP低、中、高剂量组胃黏膜组织病理有不同程度改善；各定量指标均显著逆转（
P
＜0.05）；Nrf2抑制剂ML385可显著减弱高剂量CPP对CAG大鼠上述指标的改善作用（
P
＜0.05）。
结论
2
CPP能改善CAG大鼠胃黏膜损伤，抑制氧化应激、炎症反应和细胞凋亡，其作用机制可能与激活Nrf2/HO-1信号通路有关。
Abstract
OBJECTIVE
2
To investigate the effects of
Codonopsis pilosula
polysaccharide （CPP） regulating the nuclear factor-erythroid 2-related factor 2 （Nrf2）/heme oxygenase-1 （HO-1） signaling pathway on gastric mucosal injury in rats with chronic atrophic gastritis （CAG）.
METHODS
2
Rats were randomly divided into control group， model group， CPP low-dose， medium-dose and high-dose groups （CPP 10， 20， 40 mg/kg）， and ML385 group （Nrf2 inhibitor ML385 30 mg/kg+CPP 40 mg/kg）， 10 rats per group. CAG rat model was established using
N
-methyl-
N
′- nitro-
N
-nitrosoguanidine combined with irregular diet， then they were given drugs for consecutive 6 weeks. HE staining was used to observe the pathological changes in gastric tissue morphology； the levels of serum gastrin （GAS）， motilin （MTL）， pepsin （PP）， as well as tumor necrosis factor-α （TNF-α）， interleukin-8 （IL-8） malondialdehyde （MDA） and superoxide dismutase （SOD） in gastric mucosal tissue were detected； TUNEL assay was used to observe gastric mucosal tissue cell apoptosis； immunohistochemical assay was adopted to observe the expressions of Nrf2 and recombinant Bcl2 associated X protein （Bax） in gastric mucosal tissue； Western blot was used to detect the expressions of Nrf2， HO-1， Bax and Bcl-2 proteins in gastric mucosal tissue.
RESULTS
2
Compared with the control group， the gastric mucosal tissue was damaged； the levels of GAS， MTL， PP and SOD， and the protein expressions of Nrf2， HO-1 and Bcl-2 were significantly reduced in model group （
P
＜0.05）， while the levels of MDA， TNF-α and IL
-8， the cell apoptosis index， and the protein expression of Bax were significantly increased （
P
＜0.05）. Compared with model group， CPP low-dose， medium-dose and high-dose groups showed varying degrees of improvement in gastric mucosal histopathology； the levels of the quantitative indicators were significantly reversed （
P
＜0.05）. Nrf2 inhibitor ML385 significantly attenuated the improvement effect of high-dose CPP on the above indicators in CAG rats （
P
＜0.05）.
CONCLUSIONS
2
CPP can improve gastric mucosal injury in CAG rats， and inhibit oxidative stress， inflammatory response， and cell apoptosis. The mechanism of action may be related to the activation of Nrf2/HO-1 signaling pathway.
关键词
党参多糖慢性萎缩性胃炎胃黏膜损伤Nrf2/HO-1信号通路
Keywords
chronic atrophic gastritisgastric mucosal injuryNrf2/HO-1 signaling pathway
references
YIN Y，LIANG H L，WEI N，et al. Prevalence of chronic atrophic gastritis worldwide from 2010 to 2020：an updated systematic review and meta-analysis[J]. Ann Palliat Med，2022，11（12）：3697-3703.
LIN X K，WANG W L. Analysis of high risk factors for chronic atrophic gastritis[J]. Saudi J Gastroenterol，2023，29（2）：127-134.
WENG J，WU X F，SHAO P，et al. Medicine for chronic atrophic gastritis：a systematic review，meta- and network pharmacology analysis[J]. Ann Med，2023，55（2）：2299352.
CHEN B，DONG X Q，ZHANG J L，et al. Effects of oxidative stress regulation in inflammation-associated gastric cancer progression treated using traditional Chinese medicines：a review[J]. Medicine，2023，102（46）：e36157.
HE R P，MA R Y，JIN Z，et al. Proteomics and metabolomics unveil Codonopsis pilosula（Franch.）Nannf. ameliorates gastric precancerous lesions via regulating energy metabolism[J]. Front Pharmacol，2022，13：933096.
LUAN F，JI Y F，PENG L X，et al. Extraction，purification，structural characteristics and biological properties of the polysaccharides from Codonopsis pilosula：a review[J]. Carbohydr Polym，2021，261：117863.
王涛，葛睿，杨飞，等. 党参提取物对大鼠胃黏膜损伤的保护作用[J]. 中药药理与临床，2015，31（4）：138-141.
WANG T，GE R，YANG F，et al. Protection of the extract of Codonopsis pilosula on rat gastric mucosal injury[J]. Pharmacol Clin Chin Mater Med，2015，31（4）：138-141.
ZOU Y F，YAN H，LI C Y，et al. A pectic polysaccharide from Codonopsis pilosula alleviates inflammatory response and oxidative stress of aging mice via modulating intestinal microbiota-related gut-liver axis[J]. Antioxidants，2023，12（9）：1781.
XU L L，GAO Y R，HU M，et al. Edaravone dexborneol protects cerebral ischemia reperfusion injury through activating Nrf2/HO-1 signaling pathway in mice[J]. Fundam Clin Pharmacol，2022，36（5）：790-800.
LIU S，SHU L，YANG J Y，et al. Rhein exhibits anti-inflammatory effects in chronic atrophic gastritis via Nrf2 and MAPK signaling[J]. Turk J Gastroenterol，2023，34（5）：525-532.
马竞，何文龙，高重阳，等. 党参多糖介导Nrf2通路对缺氧缺血性脑损伤的抗氧化和神经保护作用[J]. 中国临床解剖学杂志，2019，37（4）：403-408.
MA J，HE W L，GAO C Y，et al. Antioxidant and neuroprotective effects of Codonopsis pilosula polysaccharides on hypoxic-ischemic brain injury induced by Nrf2 pathway[J]. Chin J Clin Anat，2019，37（4）：403-408.
游绍伟，易旭，赵琦，等. 基于NF-κB信号通路探讨萎胃通调汤对大鼠慢性萎缩性胃炎的作用机制[J]. 中国实验方剂学杂志，2023，29（2）：88-97.
YOU S W，YI X，ZHAO Q，et al. Mechanism of Weiwei tongtiao decoction against chronic atrophic gastritis in rats：based on NF-κB signaling pathway[J] Chin J Exp Tradit Med Formul，2023，29（2）：88-97.
SUN Y Y，ZHU H J，ZHAO R Y，et al. Remote ischemic conditioning attenuates oxidative stress and inflammation via the Nrf2/HO-1 pathway in MCAO mice[J]. Redox Biol，2023，66：102852.
SUN L，JIN X L，HUANG L，et al. Risk of progression in patients with chronic atrophic gastritis：a retrospective study[J]. Front Oncol，2022，12：942091.
LI F，YANG Y P，GE J L，et al. Multi-omics revealed the mechanisms of Codonopsis pilosula aqueous extract in improving UC through blocking abnormal activation of PI3K/Akt signaling pathway[J]. J Ethnopharmacol，2024，319（Pt 2）：117220.
XU Q H，CUI F，LI X D，et al. Dangshen huangjiu prevents gastric mucosal injury and inhibits Akt/NF-κB pathway[J]. Food Funct，2023，14（17）：7897-7911.
MENG X Q，KUANG H X，WANG Q H，et al. A poly-saccharide from Codonopsis pilosula roots attenuates carbon tetrachloride-induced liver fibrosis via modulation of TLR4/NF-κB and TGF-β1/Smad3 signaling pathway[J]. Int Immunopharmacol，2023，119：110180.
YAN Z P，XU T T，XU Y X，et al. Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β-catenin signaling pathway[J]. Exp Ther Med，2021，22（2）：878.
HE Y，LIU H H，ZHOU X L，et al. Rutaecarpine ameliorates murine N-methyl-N′-nitro-N-nitrosoguanidine-induced chronic atrophic gastritis by sonic hedgehog pathway[J]. Molecules，2023，28（17）：6294.
ALOMAIR M K，ALABDULADHEEM L S，ALMAJED M A，et al. Achillea millefolium essential oil mitigates peptic ulcer in rats through Nrf2/HO-1 pathway[J]. Molecules，2022，27（22）：7908.
PEI J，WU M D，CAI S Y，et al. The protective effect of ursolic acid on unilateral ureteral obstruction in rats by activating the Nrf2/HO-1 antioxidant signaling pathway[J]. Comput Intell Neurosci，2022，2022：3690524.
BALAHA M F，ALMALKI Z S，ALAHMARI A K，et al. AMPK/mTOR-driven autophagy & Nrf2/HO-1 cascade modulation by amentoflavone ameliorates indomethacin-induced gastric ulcer[J]. Biomed Pharmacother，2022，151：113200.
XIE L，LUO M Y，LI J L，et al. Gastroprotective mechanism of modified LVdou gancao decoction on ethanol-induced gastric lesions in mice：involvement of Nrf-2/HO-1/NF-κB signaling pathway[J]. Front Pharmacol，2022，13：953885.
0
Views
0
下载量
0
CSCD
- L-PDFDownload
- WORDDownload
- Meta-XMLDownload
- BibTeXDownload
- EndnoteDownload
- RefMan Download
- NoteExpressDownload
- NoteFirstDownload
Publicity Resources
Related Articles
Related Author
Related Institution
- Address：8th Floor, Sihuan Building, No. 129 Daping Main Street, Yuzhong District, Chongqing, China Postal code：400042
- Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号
公网安备50010302001817 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.