Evaluation of antioxidant activity compounds extracted from the pine needle of <italic style="font-style: italic">Cedrus deodara</italic> by HPLC-DPPH screening method

DAI Xiaoyan; SHEN Wei; ZHANG Yan; ZHAO Yanping

doi:10.6039/j.issn.1001-0408.2024.16.10

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Evaluation of antioxidant activity compounds extracted from the pine needle of Cedrus deodara by HPLC-DPPH screening method

更新时间：2024-08-23

- Evaluation of antioxidant activity compounds extracted from the pine needle of Cedrus deodara by HPLC-DPPH screening method
- ZHONGGUO YAOFANG Vol. 35, Issue 16, Pages: 1998-2001(2024)
- 作者机构：
  
  甘肃省肿瘤医院药学部，兰州　730050
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.16.10
  CLC： R917;R284;R285
- Published：30 August 2024，
  
  Received：11 April 2024，
  
  Revised：17 July 2024，
- 稿件说明：
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戴晓雁,沈薇,张燕等.HPLC-DPPH法评价雪松松针抗氧化活性成分 ^Δ[J].中国药房,2024,35(16):1998-2001.

DAI Xiaoyan,SHEN Wei,ZHANG Yan,et al.Evaluation of antioxidant activity compounds extracted from the pine needle of Cedrus deodara by HPLC-DPPH screening method[J].ZHONGGUO YAOFANG,2024,35(16):1998-2001.
戴晓雁,沈薇,张燕等.HPLC-DPPH法评价雪松松针抗氧化活性成分 ^Δ[J].中国药房,2024,35(16):1998-2001. DOI： 10.6039/j.issn.1001-0408.2024.16.10.

DAI Xiaoyan,SHEN Wei,ZHANG Yan,et al.Evaluation of antioxidant activity compounds extracted from the pine needle of Cedrus deodara by HPLC-DPPH screening method[J].ZHONGGUO YAOFANG,2024,35(16):1998-2001. DOI： 10.6039/j.issn.1001-0408.2024.16.10.

摘要

目的

评价雪松松针抗氧化活性成分。

方法

以维生素C（VC）和没食子酸为阳性对照，采用高效液相色谱（HPLC）法测定1，1-二苯基-2-苦肼基（DPPH）溶液加入雪松松针抗氧化活性成分后DPPH峰面积的衰减情况，并通过计算DPPH自由基的清除率和半数抑制浓度（IC

），来评价雪松松针3种有效部位及8个单体成分的抗氧化活性。采用Agilent Eclipse Plus-C

（250 mm×4.6 mm，5 μm）为色谱柱，乙腈-水（62∶38，

）为流动相；检测波长为517 nm；流速为1.0 mL/min；柱温为25 ℃；进样量为20 μL。

结果

雪松松针各有效部位清除DPPH自由基活性的能力大小依次为总黄酮＞总多酚＞总木脂素，IC

分别为76.10、100.50、115.40 μg/mL。与阳性对照比较，除山柰酚-3-

-D-葡萄糖苷外，7个单体化合物清除DPPH自由基活性的能力大小依次为二氢杨梅素＞金丝桃苷＞原儿茶酸＞没食子酸＞阿魏酸＞杨梅素＞山柰酚-3-

-（6″-

O-E-

阿魏酰基）-

-D-吡喃葡萄糖苷＞VC＞异鼠李素，IC

分别为16.50、19.40、23.73、27.24、32.10、32.20、47.60、93.20、297.20 μg/mL。

结论

建立了HPLC-DPPH法用于评价雪松松针抗氧化活性；雪松松针具有较强的抗氧化活性，其中有效部位总黄酮、总多酚及单体成分二氢杨梅素、金丝桃苷等是具有开发前景的纯天然抗氧化剂。

Abstract

OBJECTIVE

To evaluate the antioxidant activity compounds extracted from the pine needle of

Cedrus deodara

METHODS

Using vitamin C （VC） and gallic acid as positive control， HPLC method was employed to determine the changes of the DPPH peak area after antioxidant compounds from pine needle of

C. deodara

were treated with 1， 1-diphenyl-2-picrylhydrazyl radical （DPPH）. Then， the antioxidant activities of 3 active parts and 8 monomer compositions

from pine needle of

C. deodara

were evaluated by calculating the inhibition rate of DPPH free radicals and the half inhibitory concentration （IC

）. HPLC conditions were using Agilent Eclipse Plus-C

column （250 mm × 4.6 mm， 5 μm） with acetonitrile-water （62∶38，

） as the mobile phase. The detection wavelength was 517 nm； the flow rate was 1.0 mL/min； the column temperature was 25 ℃； the injection volume was 20 μL.

RESULTS

The order of DPPH free radical inhibition activity of active parts from pine needle of

C. deodara

was total flavonoids＞total polyphenols＞total lignans， with IC

of 76.10， 100.50 and 115.40 μg/mL， respectively. Compared with positive control， except for kaempferol-3-

-D-glucoside， the sequence of seven monomer compounds for DPPH free radical inhibition activity was dihydromyricetin＞hypericin＞protocatechuic acid＞gallic acid＞ferulic acid＞myricetin＞kaempferol-3-

-（6″-

-feruloyl）-

-D-glucopyranoside＞VC＞isohamnin， with IC

of 16.50， 19.40， 23.73， 27.24， 32.10， 32.20， 47.60， 93.20 and 297.20 μg/mL， respectively.

CONCLUSIONS

HPLC-DPPH method is established to evaluate the antioxidant activity of pine needle of

C. deodara

. The pine needle of

C. deodara

has strong antioxidant activity， among which the effective compounds of total flavonoids， total polyphenols， monomer dihydromyricetin and hypericin are natural antioxidants with development prospects.

关键词

雪松松针抗氧化活性有效部位化学成分HPLC-DPPH

Keywords

antioxidant activityeffective fractionchemical constituentHPLC-DPPH

references

郑万钧，傅立国.中国植物志：第七卷[M].北京：科学出版社，1978：167-203.

ZHENG W J，FU L G. Flora of China：volume 7[M]. Beijing：Science Press，1978：167-203.

白朝辉，石晓峰，刘东彦，等. 雪松松针的化学成分及药理作用研究概述[J]. 中国药师，2012，15（12）：1791-1793.

BAI Z H，SHI X F，LIU D Y，et al. Summary of chemical constituents and pharmacological effects of cedar pine needles[J]. China Pharm，2012，15（12）：1791-1793.

WU Y P，LIANG X，LIU X Y，et al. Cedrus deodara pine needle as a potential source of natural antioxidants：bioactive constituents and antioxidant activities[J]. J Funct Foods，2015，14：605-612.

刘东彦，石晓峰，胡鹏斌，等. 大孔吸附树脂富集纯化雪松松针总黄酮工艺的优化[J]. 中成药，2017，39（2）：308-311.

LIU D Y，SHI X F，HU P B，et al. Optimization of enrichment and purification of total flavonoids from pine needles of Cedrus deodara by macroporous adsorption resin[J]. Chin Tradit Pat Med，2017，39（2）：308-311.

石晓峰，沈薇，宁红霞，等. 雪松松针总多酚的纯化工艺和抗氧化活性研究[J]. 天然产物研究与开发，2016，28（8）：1325-1331.

SHI X F，SHEN W，NING H X，et al. Purification and anti-oxidant activity of polyphenol from pine needles of Cedrus deodara[J]. Nat Prod Res Dev，2016，28（8）：1325-1331.

李师，雷艳萍，石晓峰，等. 雪松松针总皂苷的超声提取工艺优选及体外抗肿瘤活性研究[J]. 中国药房，2016，27（31）：4406-4410.

LI S，LEI Y P，SHI X F，et al. Optimization of the ultrasonic extraction technology of total saponins from pine needle of Cedrus deodara and study on its in vitro anti-tumor activity[J]. China Pharm，2016，27（31）：4406-4410.

张军民，石晓峰，马趣环，等. 雪松松针化学成分研究：Ⅱ[J]. 中药材，2010，33（7）：1084-1086.

ZHANG J M，SHI X F，MA Q H，et al. Studies on the chemical constituents from pine needles of Cedrus deodara：Ⅱ[J]. J Chin Med Mater，2010，33（7）：1084-1086.

刘东彦，石晓峰，李冲，等. 雪松松针黄酮类化学成分的研究[J]. 中草药，2011，42（4）：631-633.

LIU D Y，SHI X F，LI C，et al. Chemical constituents of flavonoids in pine needles of Cedrus deodara[J]. Chin Tradit Herb Drugs，2011，42（4）：631-633.

石晓峰，白朝辉，刘东彦，等. 雪松松针二氯甲烷提取物的化学成分研究[J]. 中药材，2012，35（3）：404-406.

SHI X F，BAIC Z H，LIU D Y，et al. Study on the chemical constituent from the dichloromethane extract of the pine needles of Cedrus deodara[J]. J Chin Med Mater，2012，35（3）：404-406.

王璐，孙莉琼，苏航，等. 联用技术在自由基清除物筛选中的应用[J]. 中草药，2012，43（5）：1032-1036.

WANG L，SUN L Q，SU H，et al. Application of multiple techniques in free radical scavenging material screening[J]. Chin Tradit Herb Drugs，2012，43（5）：1032-1036.

王小淞，陈波，姚守拙.HPLC-DPPH 在线法筛选黄芩提取物中抗氧化活性成分[J].中草药，2009，40（增刊1）：224-227.

WANG X S，CHEN B，YAO S Z.HPLC-DPPH online method for screening antioxidant active ingredients in Scutellaria baicalensis extract [J]. Chin Tradit Herb Drugs，2009，40（Suppl. 1）：224-227.

LIU Y W，LI H B，WANG X F，et al. Evaluation of the free radical scavenging activity of Cynomorium songaricum Rupr. by a novel DPPH-HPLC method[J]. J Food Sci，2011，76（9）：C1245-C1249.

刘明钰，李敏，陈娟娟，等. 2，2-二苯基-1-苦肼基-高效液相色谱法快速筛选亚麻籽抗氧化活性成分[J]. 分析化学，2015，43（2）：245-250.

LIU M Y，LI M，CHEN J J，et al. Evaluation of antioxidant activity of flaxseed extracts by 2，2-diphenyl-1-picrylhydrazyl-high performance liquid chromatography assay[J]. Chin J Anal Chem，2015，43（2）：245-250.

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⁰

Evaluation of antioxidant activity compounds extracted from the pine needle of Cedrus deodara by HPLC-DPPH screening method

DOI：10.6039/j.issn.1001-0408.2024.16.10

摘要

Abstract

关键词

Keywords

references