Establishment and clinical application of imipenem measurement method in patients with severe infection

CHEN Yongyan; SUN Didi; HAN Wenchao; WANG Qian; ZHANG Hanjuan

doi:10.6039/j.issn.1001-0408.2024.16.15

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Establishment and clinical application of imipenem measurement method in patients with severe infection

更新时间：2025-09-23

- Establishment and clinical application of imipenem measurement method in patients with severe infection
- ZHONGGUO YAOFANG Vol. 35, Issue 16, Pages: 2023-2026(2024)
- 作者机构：
  
  郑州市第七人民医院药学部，郑州　450016
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.16.15
  CLC： R917;R978.1
- Received：19 February 2024，
  
  Revised：2024-07-17，
  
  Accepted：23 July 2024，
  
  Published：30 August 2024
- 稿件说明：
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陈永妍,孙迪迪,韩文超,等.重症感染患者亚胺培南检测方法的建立及临床应用 [J].中国药房,2024,35(16):2023-2026.

CHEN Yongyan,SUN Didi,HAN Wenchao,et al.Establishment and clinical application of imipenem measurement method in patients with severe infection[J].ZHONGGUO YAOFANG,2024,35(16):2023-2026.
陈永妍,孙迪迪,韩文超,等.重症感染患者亚胺培南检测方法的建立及临床应用 [J].中国药房,2024,35(16):2023-2026. DOI： 10.6039/j.issn.1001-0408.2024.16.15.

CHEN Yongyan,SUN Didi,HAN Wenchao,et al.Establishment and clinical application of imipenem measurement method in patients with severe infection[J].ZHONGGUO YAOFANG,2024,35(16):2023-2026. DOI： 10.6039/j.issn.1001-0408.2024.16.15.

摘要

目的

建立适用于检测重症感染患者亚胺培南血药浓度的二维液相色谱法，并应用于临床。

方法

基于全自动二维液相色谱仪建立亚胺培南血药浓度检测方法，以一维色谱柱Aston SNCB（50 mm×4.6 mm，5 μm）萃取分离目标物，再经二维色谱柱Aston SCB（250 mm×4.6 mm，5 μm）进一步分离测定。一维流动相为亚胺培南-1D移动相[乙腈-甲醇-水（15∶10∶75，

）

]

，流速为1.0 mL/min；二维流动相为72%OPI-1有机移动相（色谱级甲醇）-20%BPI-1碱性移动相[水（含20.0 mmol/L的磷酸铵，用三乙胺调pH至7.2）

]

-8%API-1酸性移动相[水（含20.0 mmol/L的磷酸铵，用磷酸调pH至3.0）

]

，流速为1.0 mL/min；柱温为40 ℃，紫外检测波长为310 nm，进样量为100 μL。洗脱程序：0～3.40 min，一维色谱柱（亚胺培南-1D移动相）；3.40～11.00 min，二维色谱柱（72%OPI-1有机移动相-20%BPI-1碱性移动相-8%API-1酸性移动相）。

结果

亚胺培南检测质量浓度的线性范围为0.171～18.570 μg/mL（

＝0.999 9），定量下限为0.171 μg/mL；回收率在93.47%～106.16%（

＝5），日内和日间精密度的RSD均低于15%（

＝5）。51例患者的亚胺培南谷浓度为0～19.57 μg/mL。

结论

所建立的方法前处理简单、快捷，进样量大，可用于重症感染患者亚胺培南血药浓度的检测。

Abstract

OBJECTIVE

To establish two-dimensional liquid chromatography method for the determination of imipenem blood concentration and apply it in clinical practice.

METHODS

The method for the determination of imipenem blood concentration was established base

d on automatic two-dimensional liquid chromatography. The targets were extracted by 1-dimensional column Aston SNCB （50 mm ×4.6 mm， 5 μm） and further separated and determined by 2-dimensional column Aston SCB （250 mm×4.6 mm， 5 μm）. The 1-dimensional mobile phase was imipenem-1D mobile phase [acetonitrile-methanol-water （15∶10∶75，

）

]

with a flow rate of 1.0 mL/min； 2-dimensional mobile phase was 72%OPI-1 organic mobile phase （chromatographic grade methanol）-20% BPI-1 alkaline mobile phase [water （containing 20.0 mmol/L ammonium phosphate， pH adjusted to 7.2 with triethylamine）

]

-8%API-1 acidic mobile phase [water （containing 20.0 mmol/L ammonium phosphate， pH adjusted to 3.0 with phosphoric acid）

]

with a flow rate of 1.0 mL/min； the column temperature was 40 ℃， UV detection wavelength was 310 nm and injection volume was 100 μL. Elution procedure： 1-dimensional column consisted of imipenem-1D mobile phase with eluting for 0-3.40 min； 2-dimensional column consisted of 72% OPI-1 organic mobile phase-20%BPI-1 alkaline mobile phase-8%API-1 acidic mobile phase with eluting for 3.40-11.00 min.

RESULTS

The linear range of imipenem was 0.171-18.570 μg/mL （

＝0.999 9） with the lower limit of quantification for 0.171 μg/mL； the recovery rate ranged from 93.47% to 106.16% （

＝5） and the RSDs of both intra-day and inter-day precision were below 15% （

＝5）. The minimum concentration of imipenem in 51 patients ranged from 0 to 19.57 μg/mL.

CONCLUSIONS

The established method is simple and fast with the large scale of sample， and can be used for the imipenem blood concentration monitoring in patients with severe infection.

关键词

Keywords

references

中国医药教育协会感染疾病专业委员会 . 抗菌药物药代动力学/药效学理论临床应用专家共识 [J ] . 中华结核和呼吸杂志， 2018 ， 41 （ 6 ）： 409 - 446 .

Infectious Disease Professional Committee of China Medical Education Association . Expert consensus on clinical application of pharmacokinetics/pharmacodynamics theory of antibacterial drugs [J ] . Chin J Tuberc Respir Dis ， 2018 ， 41 （ 6 ）： 409 - 446 .

DHAESE S ， VAN VOOREN S ， BOELENS J ， et al . Therapeutic drug monitoring of β-lactam antibiotics in the ICU [J ] . Expert Rev Anti Infect Ther ， 2020 ， 18 （ 11 ）： 1155 - 1164 .

STAŠEK J ， KELLER F ， KOČÍ V ， et al . Update on therapeutic drug monitoring of beta-lactam antibiotics in critically ill patients：a narrative review [J ] . Antibiotics ， 2023 ， 12 （ 3 ）： 568 .

DOWNES K J ， GOLDMAN J L . Too much of a good thing： defining antimicrobial therapeutic targets to minimize toxicity [J ] . Clin Pharmacol Ther ， 2021 ， 109 （ 4 ）： 905 - 917 .

GASPAR V P ， IBRAHIM S ， ZAHEDI R P ， et al . Uti-lity， promise， and limitations of liquid chromatography-mass spectrometry-based therapeutic drug monitoring in precision medicine [J ] . J Mass Spectrom ， 2021 ， 56 （ 11 ）： e4788 .

GUILHAUMOU R ， BENABOUD S ， BENNIS Y ， et al . Optimization of the treatment with beta-lactam antibiotics in critically ill patients：guidelines from the French Society of Pharmacology and Therapeutics （Société Française de Pharmacologie et Thérapeutique-SFPT） and the French Society of Anaesthesia and Intensive Care Medicine （Société Française d’Anesthésie et Réanimation-SFAR） [J ] . Crit Care ， 2019 ， 23 （ 1 ）： 104 .

蔡婕，许金娜，倪穗琴 . HPLC法测定人血浆中亚胺培南浓度及建立临床标本采样流程 [J ] . 广州医药， 2022 ， 8 （ 3 ）： 18 - 21， 26 .

CAI J ， XU J N ， NI S Q . Determination of imipenem concentration in human plasma by HPLC and establishing the sampling process of clinical specimens [J ] . Guangzhou Med J ， 2022 ， 8 （ 3 ）： 18 - 21， 26 .

李逃明，董李晨，旷达彬，等 . HPLC法测定人血浆中美罗培南与亚胺培南的浓度 [J ] . 海峡药学， 2022 ， 34 （ 7 ）： 87 - 90 .

LI T M ， DONG L C ， KUANG D B ， et al . Determination of the concentrations of meropenem and imipenem in hu-man plasma by HPLC [J ] . Strait Pharm J ， 2022 ， 34 （ 7 ）： 87 - 90 .

邓阳，徐兵，李昕，等 . 基于亚胺培南稳定性考察的治疗药物监测临床采样流程建立 [J ] . 中国临床药理学杂志， 2018 ， 4 （ 18 ）： 2207 - 2210 .

DENG Y ， XU B ， LI X ， et al . Establishing the clinical sampling process of therapeutic drug monitoring based on stability of imipenem [J ] . Chin J Clin Pharmacol ， 2018 ， 4 （ 18 ）： 2207 - 2210 .

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