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Effects of polydatin on inflammatory response in dry eye disease rats and its mechanism
- ZHONGGUO YAOFANG Vol. 35, Issue 18, Pages: 2213-2218(2024)
- 作者机构：
1.天津医科大学眼科医院/眼视光学院/眼科研究所/国家眼耳鼻喉疾病临床医学研究中心天津市分中心/天津市视网膜功能与疾病重点实验室视光中心，天津 300384
2.天津医科大学眼科医院/眼视光学院/眼科研究所/国家眼耳鼻喉疾病临床医学研究中心天津市分中心/天津市视网膜功能与疾病重点实验室屈光角膜科，天津 300384
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2024.18.05
CLC： R965Published：30 September 2024，
Received：07 March 2024，
Revised：08 July 2024，
- 稿件说明：
移动端阅览
赵丽琼,魏瑞华.虎杖苷对干眼症大鼠炎症反应的影响及机制 Δ[J].中国药房,2024,35(18):2213-2218.
ZHAO Liqiong,WEI Ruihua.Effects of polydatin on inflammatory response in dry eye disease rats and its mechanism[J].ZHONGGUO YAOFANG,2024,35(18):2213-2218.
赵丽琼,魏瑞华.虎杖苷对干眼症大鼠炎症反应的影响及机制 Δ[J].中国药房,2024,35(18):2213-2218. DOI： 10.6039/j.issn.1001-0408.2024.18.05.
ZHAO Liqiong,WEI Ruihua.Effects of polydatin on inflammatory response in dry eye disease rats and its mechanism[J].ZHONGGUO YAOFANG,2024,35(18):2213-2218. DOI： 10.6039/j.issn.1001-0408.2024.18.05.
摘要
目的
2
基于蛋白激酶A（PKA）/环磷酸腺苷反应元件结合蛋白（CREB）信号通路，探讨虎杖苷（PD）对干眼症（DED）大鼠炎症反应的影响及潜在机制。
方法
2
将雄性SD大鼠随机分为空白对照组（Normal组）、模型组（单纯DED组）、0.05%PD组、0.5%PD组和0.5%PD+PKA抑制剂H-89组（0.5%PD+H-89组），每组15只。除Normal组外，其余各组大鼠通过眼球表面注射氢溴酸东莨菪碱12.5 mg/d的方式制备DED模型；与此同时，各药物组给予相应药液（0.5%、0.05%的PD，滴眼，每天3次；1 mg/kg的H-89，腹腔注射，每天1次），共7 d。检测各组大鼠的泪液分泌量、角膜荧光素染色评分和结膜组织杯状细胞密度，观察其角膜组织病理变化，检测其角膜组织中炎症因子（肿瘤坏死因子α、白细胞介素1β、白细胞介素6）水平和PKA/CREB信号通路相关蛋白的表达情况。
结果
2
与Normal组比较，单纯DED组大鼠角膜组织上皮层增厚，基质层细胞排列紊乱、疏松且部分缺失，核间距较大，有大量炎症细胞浸润；泪液分泌量、结膜组织杯状细胞密度和PKA、CREB蛋白的磷酸化水平均显著降低，角膜荧光素染色评分和角膜组织中炎症因子水平均显著升高（
P
＜0.05）。与单纯DED组比较，0.05%PD、0.5%PD组大鼠的角膜组织病理损伤有所缓解，各定量指标均显著改善，且0.5%PD组的改善效果更优（
P
＜0.05）；而H-89可显著逆转PD对各指标的改善作用（
P
＜0.05）。
结论
2
PD可增加DED大鼠的泪液分泌量和结膜组织杯状细胞密度，减轻其角膜组织炎症反应和病理损伤，上述作用与激活PKA/CREB信号通路有关。
Abstract
OBJECTIVE
2
To investigate the effects of polydatin （PD） on the inflammatory response in dry eye disease （DED） rats and its potential mechanism based on protein kinase A （PKA）/cyclic adenosine monophosphate response element binding protein （CREB） signaling pathway.
METHODS
2
Male SD rats were randomly divided into blank control group （normal group）， model group （simple DED group）， 0.05%PD group， 0.5%PD group and 0.5%PD+PKA inhibitor H-89 group （0.5%PD+H-89 group）， with 15 rats in each group. Except for the normal group， the rats in other groups were prepared with a DED model by injecting scopolamine hydrobromide 12.5 mg/d onto the surface of eyeball. At the same time， each drug group was given corresponding liquid medicine （0.5% or 0.05% PD， eye drip， 3 times a day； 1 mg/kg H-89， intraperitoneal injection， once a day） for 7 d in total. The tear secretion， corneal fluorescein staining score and conjunctival goblet cell density of rats were detected in each group； the pathological changes of corneal tissues were observed， and the levels of inflammatory factors （tumor necrosis factor-α， interleukin-1β， interleukin-6） and the expression of PKA/CREB signaling pathway-related proteins were detected in corneal tissues.
RESULTS
2
Compared with normal group， the corneal epithelium of rats in the simple DED group was thickened， stromal layer cells were disordered and partially absent， nuclear spacing was larger， and a large number of inflammatory cells infiltrated； the tear secretion， conjunctival goblet cell density and the phosphorylation levels of PKA and CREB in corneal tissues were reduced significantly， while the corneal fluorescein staining score and the levels of inflammatory factors in corneal tissue were increased significantly （
P
＜0.05）. Compared with the simple DED group， the pathological injuries of corneal tissues of rats in 0.05%PD and 0.5%PD groups were alleviated， each quantitative index was significantly improved， and the improvement effect of 0.5%PD group was more obvious （
P
＜0.05）. H-89 could reverse the improvement effect of PD on each index significantly （
P
＜0.05）.
CONCLUSIONS
2
PD can increase tear secretion and the conjunctival goblet cell density， and reduce the inflammatory response and pathological injury of corneal tissue in DED rats. The above effects are related to the activation of PKA/CREB signaling pathway.
关键词
虎杖苷干眼症炎症反应PKA/CREB信号通路
Keywords
dry eye diseaseinflammatory responsePKA/CREB signaling pathway
references
ROLANDO M，MERAYO-LLOVES J. Management stra-tegies for evaporative dry eye disease and future perspective[J]. Curr Eye Res，2022，47（6）：813-823.
MOHAMED H B，ABD EL-HAMID B N，FATHALLA D，et al. Current trends in pharmaceutical treatment of dry eye disease：a review[J]. Eur J Pharm Sci，2022，175：106206.
NGUYEN A，KOLLURU A，BEGLARIAN T. Dry eye disease：a review of anti-inflammatory therapies[J]. Taiwan J Ophthalmol，2023，13（1）：3-12.
林忠嗣，于晓斌，张正，等. 杞菊地黄汤加减联合中药熏蒸治疗干眼症及对泪液IL-6、MMP-9水平的影响[J]. 中国实验方剂学杂志，2023，29（7）：133-138.
LIN Z S，YU X B，ZHANG Z，et al. Qiju dihuang tang combined with Chinese medicine fumigation in treatment of dry eye and its effect on levels of tear IL-6 and MMP-9[J]. Chin J Exp Tradit Med Formulae，2023，29（7）：133-138.
白雪，刘艳茹，王海. 刺槐素通过调控TLR4通路对干眼症大鼠的保护作用及其机制研究[J].世界科学技术-中医药现代化，2022，24（7）：2740-2747.
BAI X，LIU Y R，WANG H. Protective effects of acacetin on xerophthalmia rats by regulating TLR4 pathway and its mechanism[J]. Modern TCM Materia Medica-World Sci Technol，2022，24（7）：2740-2747.
LING J W，CHAN C L，HO C Y，et al. The extracts of Dendrobium alleviate dry eye disease in rat model by regulating aquaporin expression and MAPKs/NF-κB signalling[J]. Int J Mol Sci，2022，23（19）：11195.
KARAMI A，FAKHRI S，KOOSHKI L，et al. Polydatin：pharmacological mechanisms，therapeutic targets，biolo-gical activities，and health benefits[J]. Molecules，2022，27（19）：6474.
PARK B，JO K，LEE T G，et al. Polydatin inhibits NLRP3 inflammasome in dry eye disease by attenuating oxidative stress and inhibiting the NF-κB pathway[J]. Nutrients，2019，11（11）：2792.
陈敬君，马贤聪，杨泉，等. 芍药苷调控环磷酸腺苷/蛋白激酶A/环磷酸腺苷反应元件结合蛋白通路对脑卒中大鼠的影响[J]. 安徽医药，2022，26（6）：1073-1078.
CHEN J J，MA X C，YANG Q，et al. The effect of paeoniflorin on stroke rats by regulating cAMP/PKA/CREB pathway[J]. Anhui Med Pharm J，2022，26（6）：1073-1078.
RU Y S，HUANG Y，LIU H J，et al. α-Melanocyte-stimulating hormone ameliorates ocular surface dysfunctions and lesions in a scopolamine-induced dry eye model via PKA-CREB and MEK-Erk pathways[J]. Sci Rep，2015，5：18619.
姚倩，田蕴霖，周歆，等. 环孢素A滴眼液联合维生素A治疗大鼠干眼症的效果及作用机制[J]. 山东医药，2020，60（16）：26-29.
YAO Q，TIAN Y L，ZHOU X，et al. Effect of cyclosporine A eye drops combined with vitamin A on dry eye syndrome in rats and its mechanism[J]. Shandong Med J，2020，60（16）：26-29.
YAZDANI M，ELGSTØEN K B P，ROOTWELT H，et al. Tear metabolomics in dry eye disease：a review[J]. Int J Mol Sci，2019，20（15）：3755.
MESSMER E M，AHMAD S，BENITEZ DEL CASTILLO J M，et al. Management of inflammation in dry eye disease：recommendations from a European panel of experts[J]. Eur J Ophthalmol，2023，33（3）：1294-1307.
曹瑾，龚兰兰，吕旭东. 虎杖苷激活MEK/ERK信号通路改善东莨菪碱诱导的干眼症大鼠眼表功能障碍和病变[J]. 中国免疫学杂志，2024，40（5）：1089-1095.
CAO J，GONG L L，LYU X D. Polydatin activates MEK/ERK signaling pathway to improve ocular surface dysfunction and pathology in rats with scopolamine-induced dry eye disease[J]. Chin J Immunol，2024，40（5）：1089-1095.
YU L T，LIU S X，ZHOU R X，et al. Atorvastatin inhibits neuronal apoptosis via activating cAMP/PKA/p-CREB/BDNF pathway in hypoxic-ischemic neonatal rats[J]. FASEB J，2022，36（4）：e22263.
CAMMALLERI M，AMATO R，OLIVIERI M，et al. Effects of topical gabapentin on ocular pain and tear secretion[J]. Front Pharmacol，2021，12：671238.
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