Identification of chemical constituents of modified Yupingfengsan formula

LIU Cuizhen; FENG Siqi; LIN Xiujuan; LU Qin; HUANG Yusheng; ZHANG Lijuan; LI Detang

doi:10.6039/j.issn.1001-0408.2024.18.07

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Identification of chemical constituents of modified Yupingfengsan formula

更新时间：2024-09-25

- Identification of chemical constituents of modified Yupingfengsan formula
- ZHONGGUO YAOFANG Vol. 35, Issue 18, Pages: 2225-2231(2024)
- 作者机构：
  
  1.广州中医药大学第一临床医学院，广州　510405
  2.广州中医药大学岭南医学研究中心，广州　510407
  3.广东省中医临床研究院，广州　510405
  4.广州中医药大学第一附属医院耳鼻喉科，广州　510405
  5.广州中医药大学第一附属医院药学部，广州　510405
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.18.07
  CLC： R917
- Published：30 September 2024，
  
  Received：15 March 2024，
  
  Revised：18 August 2024，
- 稿件说明：
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刘翠珍,冯思琪,林秀娟等.玉屏风散加味方化学成分鉴定 ^Δ[J].中国药房,2024,35(18):2225-2231.

LIU Cuizhen,FENG Siqi,LIN Xiujuan,et al.Identification of chemical constituents of modified Yupingfengsan formula[J].ZHONGGUO YAOFANG,2024,35(18):2225-2231.
刘翠珍,冯思琪,林秀娟等.玉屏风散加味方化学成分鉴定 ^Δ[J].中国药房,2024,35(18):2225-2231. DOI： 10.6039/j.issn.1001-0408.2024.18.07.

LIU Cuizhen,FENG Siqi,LIN Xiujuan,et al.Identification of chemical constituents of modified Yupingfengsan formula[J].ZHONGGUO YAOFANG,2024,35(18):2225-2231. DOI： 10.6039/j.issn.1001-0408.2024.18.07.

摘要

目的

鉴定玉屏风散加味方的化学成分。

方法

采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术。以Waters BEH C

为色谱柱，乙腈（A）-0.1%甲酸溶液（B）为流动相进行梯度洗脱；采用加热电喷雾离子源进行正、负离子模式扫描，扫描范围为

50～1 500，喷雾电压为2 kV（正离子模式）、1.5 kV（负离子模式）。通过查阅文献收集玉屏风散加味方的化学成分信息，建立数据库；结合上述成分数据库、相关文献以及对照品的色谱、质谱信息对该方成分结构进行鉴定。结果与

结论

从玉屏风散加味方中共鉴定出114个化学成分，包括黄酮类（31个）、苯丙素类（39个）、皂苷类（5个）、萜类（8个）、色原酮类（3个）、姜辣素类（3个）等；通过与对照品比对，最终确定了8个成分，分别为木兰花碱、毛蕊异黄酮、毛蕊异黄酮葡萄糖苷、升麻素、5-

-甲基维斯阿米醇苷、亥茅酚苷、木犀草素、芒柄花素；主要涉及糖基断裂、逆狄尔斯-阿尔德重排、糖基丢失、中性分子丢失等裂解途径。

Abstract

OBJECTIVE

To identify the chemical constituents of the modified Yupingfengsan formula.

METHODS

UPLC-Q-Exac

tive Orbitrap-MS technology was adopted. The separation was performed on Waters BEH C

column with acetonitrile （A）-0.1% formic acid solution （B） as mobile phase for gradient elution. The heating electrospray ionization was used for positive and negative ion mode scanning. The scanning range was

50-1 500， and the spray voltage was 2 kV （positive ion mode） and 1.5 kV （negative ion mode）. The information of chemical constituents of modified Yupingfengsan formula was collected through literature review to establish a database； the structure of the constituent was identified based on the above database， relevant literature， and chromatography and mass spectrometry information of reference standards.

RESULTS &

CONCLUSIONS

Totally 114 chemical constituents were identified from modified Yupingfengsan formula， including 31 flavonoids， 39 phenylpropanoids， 5 saponins， 8 terpenoids， 3 chromones， 3 curcuminoids， etc. Based on the comparison of reference standards， 8 constituents were ultimately determined， including magnoflorine， calycosin， calycosin glycoside， cimifugin， 5-

-methylvisammioside， sec-

-glucosylhamaudol， luteolin and mangiferin. These constituents mainly involved glycosylation cleavage， retro Diels-Alder fragmentation， glycosylation loss， neutral molecule loss and other fragmentation pathways.

关键词

玉屏风散加味方化学成分黄酮类苯丙素类裂解规律

Keywords

chemical constituentsflavonoidsphenylpropanoidsfragmentation pattern

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