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Study on pharmacodynamic substances of anti-inflammatory effect of Zhuang medicine
Stahlianthus involucratus based on metabolism in rats- ZHONGGUO YAOFANG Vol. 35, Issue 19, Pages: 2358-2364(2024)
- 作者机构：
1.广西中医药大学药学院/广西壮瑶药重点实验室，南宁 530200
2.壮瑶药协同创新中心，南宁 530200
3.广西壮族自治区民族药资源与应用工程研究中心，南宁 530200
4.钦州市检验检测中心，广西 钦州 535000
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2024.19.07
CLC： R917;R284Published：15 October 2024，
Received：05 April 2024，
Revised：12 August 2024，
- 稿件说明：
移动端阅览
刘星晨,梁洁,黄春燕等.基于大鼠体内代谢的壮药三七姜抗炎药效物质基础研究 Δ[J].中国药房,2024,35(19):2358-2364.
LIU Xingchen,LIANG Jie,HUANG Chunyan,et al.Study on pharmacodynamic substances of anti-inflammatory effect of Zhuang medicine Stahlianthus involucratus based on metabolism in rats[J].ZHONGGUO YAOFANG,2024,35(19):2358-2364.
刘星晨,梁洁,黄春燕等.基于大鼠体内代谢的壮药三七姜抗炎药效物质基础研究 Δ[J].中国药房,2024,35(19):2358-2364. DOI： 10.6039/j.issn.1001-0408.2024.19.07.
LIU Xingchen,LIANG Jie,HUANG Chunyan,et al.Study on pharmacodynamic substances of anti-inflammatory effect of Zhuang medicine Stahlianthus involucratus based on metabolism in rats[J].ZHONGGUO YAOFANG,2024,35(19):2358-2364. DOI： 10.6039/j.issn.1001-0408.2024.19.07.
摘要
目的
2
为壮药三七姜的药效物质基础研究提供参考。
方法
2
将24只雄性SD大鼠随机分为空白组（纯化水）、给药组（三七姜醇提物，以生药量计为15.75 g/kg），每组12只。每天灌胃药液/纯化水2次，每次灌胃间隔6～8 h，连续3 d。给药结束后，收集2组大鼠血液、尿液及粪便样品。利用超高效液相色谱联用四极杆-静电场轨道阱高分辨质谱技术对三七姜醇提物中的化学成分及大鼠灌胃三七姜醇提物后的血清、尿液、粪便代谢物进行鉴定。采用多元统计分析方法筛选血清中差异代谢物，并使用MetaboAnalyst 5.0平台对代谢通路进行分析。
结果
2
从三七姜醇提物中鉴定出了38个化合物，从尿液样品中鉴定出了14个原型成分和3个代谢产物，从粪便样品中鉴定出了9个原型成分，从血清样品中鉴定出了10个原型成分和1个代谢产物。在2组大鼠血清样本中共筛选得到71个差异代谢物，其中给药组中阿魏酸、甘草素等44个差异代谢物的水平相较于空白组上调，花生四烯酸、苯乙酰谷氨酰胺等27个差异代谢物的水平相较于空白组下调。71个差异代谢物主要富集在苯丙氨酸代谢、亚油酸代谢、花生四烯酸代谢、色氨酸代谢等11条代谢通路上。
结论
2
阿魏酸、甘草素、异嗪皮啶和刺芒柄花素等成分可能是三七姜直接发挥抗炎药效作用的物质基础，三七姜可能通过影响花生四烯酸代谢和色氨酸代谢等通路发挥抗炎作用。
Abstract
OBJECTIVE
2
To provide reference for basic analysis of the pharmacodynamic substance in
Stahlianthus involucratus
.
METHODS
2
Overall 24 SD male rats were randomly divided into blank group （purified water）， and administration group （ethanol extract of
S. involucratus
， 15.75 g/kg， calculated by crude drug）， with 12 rats in each group. They were given drug liquid/purified water intragastrically， twice a day， every 6-8 h， for consecutive 3 days. After medication， the blood， urine and fecal samples were collected from two groups of rats. UPLC-Q-Exactive-MS technology was used to identify the chemical constituents in the ethanol extract of
S. involucratus
， and metabolites in the blood， urine and fecal of rats after intragastrical administration of the ethanol extract of
S. involucratus
. Multivariate statistical analysis was employed to screen various serum metabolites. Metabolic pathways were analyzed by MetaboAnalyst 5.0 platform.
RESULTS
2
A total of 38 chemical constituents were identified from the ethanol extract of
S. involucratus
， including fourteen prototype components and three metabolites identified from urine samples， nine prototype components identified from fecal samples， and ten prototype components and one metabolite identified f
rom serum samples. A total of 71 differential metabolites were screened from two groups of rat serum samples， of which 44 differential metabolites， such as ferulic acid， glycyrrhizin， were up-regulated and 27 differential metabolites， such as arachidonic acid， phenylacetylglutamine， were down-regulated. The 71 differential metabolites were mainly enriched in 11 metabolic pathways， including phenylalanine metabolism， linoleic acid metabolism， arachidonic acid metabolism， and tryptophan metabolism.
CONCLUSIONS
2
Ferulic acid， liquiritigenin， isofraxidin and formononetin may be the material basis that directly exert pharmacological effects of
S. involucratus
.
S. involucratus
may exert anti-inflammatory effects by affecting metabolic pathways， including arachidonic acid metabolism and tryptophan metabolism.
关键词
三七姜化学成分药效物质体内代谢炎症UPLC-Q-Exactive-MS技术
Keywords
chemical constituentspharmacodynamic substancesinternal metabolisminflammationUPLC-Q-Exactive-MS technology
references
广西壮族自治区食品药品监督管理局.广西壮族自治区壮药质量标准：第二卷：2011年版[M]. 南宁：广西科学技术出版社，2011：12.
Guangxi Zhuang Autonomous Region Food and Drug Administration. Guangxi Zhuang Autonomous Region Zhuang medicine quality standards：volume 2：2011 edition [M]. Nanning：Guangxi Science and Technology Press，2011：12.
中国科学院中国植物志编辑委员会. 中国植物志：第四十六卷[M]. 北京：科学出版社，1981：44.
Editorial Committee of the Flora of China，Chinese Aca- demy of Sciences. Flora of China：volume 46[M]. Beijing：Science Press，1981：44.
谢珍珍，杨颖欣，梁洁，等. 壮药姜叶三七的化学成分鉴别研究[J]. 广西中医药大学学报，2015，18（3）：49-51.
XIE Z Z，YANG Y X，LIANG J，et al. Identification of chemical constituents of Zhuang medicine Stahlianthus involucratus[J]. J Guangxi Univ Chin Med，2015，18（3）：49-51.
曾立威，唐春燕，徐勤.三七姜挥发油成分的GC-MS分析与体外抗肿瘤活性研究[J].华夏医学，2017，30（4）：33-38.
ZENG L W，TANG C Y，XU Q. GC-MS analysis of components of essential oil of Stahlianthus involucratus and its in vitro antitumor activity[J]. Acta Med Sin，2017，30（4）：33-38.
LI W K，WU B M，WANG Y G，et al. The potential antioxidant activity and characterization of bioactive compounds of Stahlianthus involucratus[J]. Biomed Res Int，2021，2021：9490162.
陈成，刘毅，许汉林.土田七醇提物抑制肝癌细胞增殖活性部位筛选及其对肝癌细胞侵袭迁移的影响[J]. 湖南中医药大学学报，2022，42（6）：930-933.
CHEN C，LIU Y，XU H L. Screening of active fractions from the alcohol extract of Stahlianthus involucratus and its effect on invasion and migration of hepatocellular carcinoma cells[J]. J Hunan Univ Chin Med，2022，42（6）：930-933.
陈成，李锦周，刘毅，等. 土田七醇提物对实验性糖尿病小鼠血糖、血脂的影响及安全性研究[J]. 湖北中医药大学学报，2022，24（3）：5-8.
CHEN C，LI J Z，LIU Y，et al. Effects of ethanolic extract from Stahlianthus involucratus serum glucose and lipids of experimental diabetic mice and its safety evaluation[J]. J Hubei Univ Chin Med，2022，24（3）：5-8.
刘新燕，王羽，尚峰，等.基于Nrf2/ARE信号通路研究姜叶三七挥发油对氧化低密度脂蛋白诱导人脐静脉内皮细胞损伤的保护作用[J]. 环球中医药，2022，15（4）：566-576.
LIU X Y，WANG Y，SHANG F，et al. The protective effect of essential oil of rhizome from Stahlianthus involucratus on ox-LDL-induced HUVECs damage based on Nrf2/ARE signaling pathway[J]. Glob Tradit Chin Med，2022，15（4）：566-576.
余胜.基于TXNIP/NLRP3通路对透脓散及其单体阿魏酸抑制溃疡性结肠炎的实验和临床研究[D].南京：南京中医药大学，2022.
YU S. Experimental and clinical study on the inhibition of ulcerative colitis by Tounong san and its monomer ferulic acid based on TXNIP/NLRP3 pathway[D]. Nanjing： Nanjing University of Chinese Medicine，2022.
ZHU X G，SHI J，LI H C. Liquiritigenin attenuates high glucose-induced mesangial matrix accumulation，oxidative stress，and inflammation by suppression of the NF-κB and NLRP3 inflammasome pathways[J]. Biomed Pharmacother，2018，106：976-982.
QIN M M，GUO A L，LI F，et al. Liquiritigenin enhances cyclic adenosine monophosphate production to mitigate inflammation in dendritic cells[J]. Int J Immunopathol Pharmacol，2021，35：20587384211038098.
HE S，ZHANG T，WANG Y Y，et al. Isofraxidin attenuates dextran sulfate sodium-induced ulcerative colitis through inhibiting pyroptosis by upregulating Nrf2 and reducing reactive oxidative species[J]. Int Immunopharmacol，2024，128：111570.
FENG Z H，GU L W，LIN J，et al. Formononetin protects against Aspergillus fumigatus keratitis：targeting inflammation and fungal load[J]. Int Immunopharmacol，2024，132：112046.
KIKUT J，KOMORNIAK N，ZIĘTEK M，et al. Inflammation with the participation of arachidonic （AA） and linoleic acid （LA） derivatives （HETEs and HODEs） is necessary in the course of a normal reproductive cycle and pregnancy[J]. J Reprod Immunol，2020，141：103177.
CERVENKA I，AGUDELO L Z，RUAS J L. Kynurenines：tryptophan’s metabolites in exercise，inflammation，and mental health[J]. Science，2017，357（6349）：eaaf9794.
ISLAM J，SATO S，WATANABE K，et al. Dietary tryptophan alleviates dextran sodium sulfate-induced colitis through aryl hydrocarbon receptor in mice[J]. 2017，42：43-50.
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