Effect of Runzao zhiyang capsule on gut microbiota and short-chain fatty acids in eczema rats

HU Yinxia; CHEN Zizhao; LIU Changshun; HAO Yong

doi:10.6039/j.issn.1001-0408.2024.19.09

您当前的位置：

首页 >

文章列表页 >

Effect of Runzao zhiyang capsule on gut microbiota and short-chain fatty acids in eczema rats

更新时间：2024-10-11

- Effect of Runzao zhiyang capsule on gut microbiota and short-chain fatty acids in eczema rats
- ZHONGGUO YAOFANG Vol. 35, Issue 19, Pages: 2372-2377(2024)
- 作者机构：
  
  1.中国人民解放军南部战区总医院消化内科，广州　510010
  2.广东药科大学临床医学院，广州　510006
  3.南方医科大学中医药学院，广州　510515
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.19.09
  CLC： R965;R285
- Published：15 October 2024，
  
  Received：09 April 2024，
  
  Revised：21 August 2024，
- 稿件说明：
移动端阅览
胡银霞,陈梓钊,刘昌顺等.润燥止痒胶囊对湿疹大鼠肠道菌群及短链脂肪酸的影响 ^Δ[J].中国药房,2024,35(19):2372-2377.

HU Yinxia,CHEN Zizhao,LIU Changshun,et al.Effect of Runzao zhiyang capsule on gut microbiota and short-chain fatty acids in eczema rats[J].ZHONGGUO YAOFANG,2024,35(19):2372-2377.
胡银霞,陈梓钊,刘昌顺等.润燥止痒胶囊对湿疹大鼠肠道菌群及短链脂肪酸的影响 ^Δ[J].中国药房,2024,35(19):2372-2377. DOI： 10.6039/j.issn.1001-0408.2024.19.09.

HU Yinxia,CHEN Zizhao,LIU Changshun,et al.Effect of Runzao zhiyang capsule on gut microbiota and short-chain fatty acids in eczema rats[J].ZHONGGUO YAOFANG,2024,35(19):2372-2377. DOI： 10.6039/j.issn.1001-0408.2024.19.09.

摘要

目的

研究润燥止痒胶囊（RZZY）对湿疹大鼠肠道菌群及其代谢物短链脂肪酸（SCFA）的影响，以揭示RZZY对湿疹的改善作用及可能机制。

方法

将大鼠分为正常组、模型组、地氯雷他定组（1.5 mg/kg）和RZZY低、高剂量组（0.6、1.2 g/kg）。除正常组外，其余各组大鼠采用2，4-二硝基氯苯溶液涂抹脱毛区域致敏以构建湿疹模型，并灌胃相应药物，每天1次，连续2周。给药完成后，检测大鼠皮肤厚度，并进行湿疹面积及严重度指数（EASI）评分和病理评分；基于16S rDNA测序分析大鼠肠道菌群结构；采用气相色谱-质谱联用仪检测大鼠粪便中SCFA（乙酸、丙酸和丁酸）含量；检测大鼠结肠和皮肤组织中信号转导及转录激活因子3（STAT3）的表达水平。

结果

RZZY可明显减轻湿疹大鼠皮肤红肿、溃烂症状，显著减小/降低大鼠EASI、皮肤厚度、病理评分（

＜0.05）；显著升高湿疹大鼠肠道菌群ACE、Sobs和Shannon指数（

＜0.05），促进大鼠肠道菌群向正常结构回调；提高湿疹大鼠肠道厚壁菌门和毛螺菌属、乳杆菌属、另枝菌属丰度，降低拟杆菌门/属丰度；显著升高湿疹

大鼠粪便中丙酸、丁酸含量（

＜0.05）；显著下调结肠和皮肤组织中STAT3蛋白表达（

＜0.05）。

结论

RZZY可通过调节肠道菌群提高SCFA水平来抑制STAT3表达，从而发挥抗炎作用，进而改善湿疹大鼠的皮肤炎症。

Abstract

OBJECTIVE

To study the effect of Runzao zhiyang capsule （RZZY） on gut microbiota and its metabolites short-chain fatty acids （SCFA） in eczema rats， in order to reveal the improvement effect and possible mechanism of RZZY on eczema.

METHODS

The rats were divided into normal group， model group， desloratadine group （1.5 mg/kg）， and RZZY low-dose and high-dose groups （0.6， 1.2 g/kg）. Except for the normal group， the eczema model was established in other groups by applying the 2，4-dinitrochlorobenzene solution to the hair removal area to sensitize it； they were given relevant drugs intragastrically， once a day， for consecutive 2 weeks. After medication， the skin thickness of rats was measured， and the eczema area and severity index （EASI） scoring and pathology scoring were performed； the structure of gut microbiota was analyzed by 16S rDNA sequencing. The contents of SCFA （including acetic acid， propionic acid， and butyric acid） in feces were determined by gas phase-mass spectrometry. The expressions of signal transduction and activator of transcription 3 （STAT3） in the colon and skin were detected.

RESULTS

RZZY significantly alleviated skin swelling and ulceration and reduced EASI， skin thickness and pathological score in eczema rats （

＜0.05）. RZZY significantly increased ACE， Sobs and Shannon indexes （

＜0.05） and promoted the microbiota of eczema rats to return to normal structure. RZZY increased the abundance of Firmicutes and

Lachnospira

，

Lactobacillus

and

Alistipes

， while decreased the abundance of Bacteroidetes and

Bacteroides

. RZZY significantly increased the contents of propionic

acid and butyric acid in the feces of eczema rats （

＜0.05）， while significantly down-regulated the expressions of STAT3 protein in the colon and skin （

＜0.05）.

CONCLUSIONS

RZZY can improve SCFA levels by regulating gut microbiota， thereby inhibiting STAT3 expression， playing an anti-inflammatory role， and improving skin inflammation in eczema rats.

关键词

润燥止痒胶囊湿疹肠道菌群短链脂肪酸

Keywords

eczemagut microbiotashort-chain fatty acid

references

胡辉莹，胡银霞，刘昌顺，等. 网络药理学联合动物实验探讨润燥止痒胶囊治疗湿疹的作用机制[J]. 中国临床解剖学杂志， 2023， 41（5）： 572-577.

HU H Y， HU Y X， LIU C S， et al. Network pharmacology combined with animal experiment to explore the mechanism of action of Runzao zhiyang capsule in treating eczema[J]. Chin J Clin Anat， 2023， 41（5）： 572-577.

HUANG D， CHEN K， ZHANG F R， et al. Efficacy and safety of Runzao zhiyang capsule on chronic eczema： a multiple-center， randomized， double-blind， placebo-controlled clinical study[J]. J Dermatolog Treat， 2019， 30（7）： 677-684.

MARRS T， FLOHR C. The role of skin and gut microbiota in the development of atopic eczema[J]. Br J Dermatol， 2016， 175（Suppl. 2）： 13-18.

TA L D H， CHAN J C Y， YAP G C， et al. A compromised developmental trajectory of the infant gut microbiome and metabolome in atopic eczema[J]. Gut Microbes， 2020， 12（1）： 1-22.

黎豫川，张远哲，杨元凤，等. 白术-木香药对对脾虚腹泻型肠易激综合征大鼠肠道菌群与短链脂肪酸代谢的调节作用[J]. 中国药房， 2024， 35（3）： 304-310.

LI Y C， ZHANG Y Z， YANG Y F， et al. Regulatory effects of couplet medicinals of Atractylodes macrocephala-Aucklandia lappa on gut microbiota and short-chain fatty acid metabolism in the irritable bowel syndrome rat with spleen deficiency and diarrhea[J]. China Pharm， 2024， 35（3）： 304-310.

KIM H K， RUTTEN N B， BESSELING-VAN DER VAART I， et al. Probiotic supplementation influences faecal short chain fatty acids in infants at high risk for eczema[J]. Benef Microbes， 2015， 6（6）： 783-790.

HUANG I H， CHUNG W H， WU P C， et al. JAK-STAT signaling pathway in the pathogenesis of atopic dermatitis： an updated review[J]. Front Immunol， 2022， 13： 1068260.

ZHONG W B， WU K H， LONG Z N， et al. Gut dysbiosis promotes prostate cancer progression and docetaxel resistance via activating NF-κB-IL6-STAT3 axis[J]. Microbiome， 2022， 10（1）： 94.

XIA T， LIANG X， LIU C S， et al. Network pharmaco- logy integrated with transcriptomics analysis reveals Ermiao wan alleviates atopic dermatitis via suppressing MAPK and activating the EGFR/AKT signaling[J]. Drug Des Devel Ther， 2022， 16： 4325-4341.

赵辨. 湿疹面积及严重度指数评分法[J]. 中华皮肤科杂志， 2004， 37（1）： 3-4.

ZHAO B. Eczema area and severity index scoring method[J]. Chin J Dermatol， 2004， 37（1）： 3-4.

景明夷. 文氏马齿苋汤治疗急性湿疹（湿热蕴肤证）临床观察及机制研究[D]. 成都：成都中医药大学， 2023.

JING M Y. Clinical observation on Wen’s machixian decoction in treating acute eczema with the syndrome of damp heat accumulating skin and its mechanism[D]. Chengdu： Chengdu University of TCM， 2023.

刘昌顺，夏婷，魏小涵，等. 基于对药动学及血清SCFA的影响研究葛根芩连汤对腹泻小型猪的作用[J]. 世界科学技术-中医药现代化， 2021， 23（3）： 678-685.

LIU C S， XIA T， WEI X H， et al. Effect of Gegen qinlian decoction on diarrheal miniature pigs was studied basing on the change of pharmacokinetics and serum SCFA[J]. Mod Tradit Chin Med Mater Med World Sci Technol， 2021， 23（3）： 678-685.

LIU C S， LIANG X， WEI X H， et al. Gegen qinlian decoction treats diarrhea in piglets by modulating gut microbiota and short-chain fatty acids[J]. Front Microbiol， 2019， 10： 825.

刘欣，张广中，肖士菊，等. 银屑病血热证与血燥证肠道菌群特征研究[J]. 世界科学技术-中医药现代化， 2021， 23（7）： 2480-2486.

LIU X， ZHANG G Z， XIAO S J， et al. Study on characteristics of gut microbiota in blood-hest syndrome and blood-dryness syndrome of psoriasis[J]. Mod Tradit Chin Med Mater Med World Sci Technol， 2021， 23（7）： 2480-2486.

CHEUNG M K， YUE G G L， LAUW S， et al. Alterations in gut microbiota of esophageal squamous cell carcinoma patients[J]. J Gastroenterol Hepatol， 2022， 37（10）： 1919-1927.

NEYRINCK A M， RODRIGUEZ J， TAMINIAU B， et al. Constipation mitigation by rhubarb extract in middle-aged adults is linked to gut microbiome modulation： a double-blind randomized placebo-controlled trial[J]. Int J Mol Sci， 2022， 23（23）： 14685.

ZHONG X F， ZHANG Y B， HUANG G D， et al. Proteomic analysis of stachyose contribution to the growth of Lactobacillus acidophilus CICC22162[J]. Food Funct， 2018， 9（5）： 2979-2988.

SZALUS K， TRZECIAK M， NOWICKI R J. JAK-STAT inhibitors in atopic dermatitis from pathogenesis to clinical trials results[J]. Microorganisms， 2020， 8（11）： 1743.

TIAN L R， LI C， XIANG L M， et al. T52 attenuates oncogenic STAT3 signaling and suppresses osteosarcoma[J]. Phytomedicine， 2023， 114： 154799.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Regulatory effects of couplet medicinals of Atractylodes macrocephala-Aucklandia lappa on gut microbiota and short-chain fatty acid metabolism in the irritable bowel syndrome rat with spleen deficiency and diarrhea

Effects of Guizhi Fuling Capsules and Its Main Components on Intestinal Flora of Primary Dysmenorrhea Model Rats

Related Author

LI Yuchuan

ZHANG Yuanzhe

YANG Yuanfeng

CHEN Lida

XU Xianmei

DUAN Kun

GAO Xia

GENG Ting

Related Institution

Dept. of Pharmacy， the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine

School of Basic Medicine， Guizhou University of Traditional Chinese Medicine

Address：8th Floor, Sihuan Building, No. 129 Daping Main Street, Yuzhong District, Chongqing, China Postal code：400042
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰