Content determination and chemometric analysis of 8 components in <italic style="font-style: italic">Smilax glabra</italic>

ZOU Huiliang; WANG Xincai; CHEN Ke; CUI Hong; MENG Lingjie; LIU Bailian; CHEN Min

doi:10.6039/j.issn.1001-0408.2024.19.10

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Content determination and chemometric analysis of 8 components in Smilax glabra

更新时间：2024-10-11

- Content determination and chemometric analysis of 8 components in Smilax glabra
- ZHONGGUO YAOFANG Vol. 35, Issue 19, Pages: 2378-2382(2024)
- 作者机构：
  
  1.湖州市食品药品检验研究院，浙江湖州　313000
  2.遵义医科大学生命科学研究院，贵州遵义　563000
  3.暨南大学药学院中药及天然药物研究所，广州　510632
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.19.10
  CLC： R917;R284.1
- Published：15 October 2024，
  
  Received：16 April 2024，
  
  Revised：16 August 2024，
- 稿件说明：
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邹惠亮,王新财,陈柯等.土茯苓中8个成分的含量测定及化学计量学分析 ^Δ[J].中国药房,2024,35(19):2378-2382.

ZOU Huiliang,WANG Xincai,CHEN Ke,et al.Content determination and chemometric analysis of 8 components in Smilax glabra[J].ZHONGGUO YAOFANG,2024,35(19):2378-2382.
邹惠亮,王新财,陈柯等.土茯苓中8个成分的含量测定及化学计量学分析 ^Δ[J].中国药房,2024,35(19):2378-2382. DOI： 10.6039/j.issn.1001-0408.2024.19.10.

ZOU Huiliang,WANG Xincai,CHEN Ke,et al.Content determination and chemometric analysis of 8 components in Smilax glabra[J].ZHONGGUO YAOFANG,2024,35(19):2378-2382. DOI： 10.6039/j.issn.1001-0408.2024.19.10.

摘要

目的

为土茯苓药材的质量控制提供依据。

方法

采用超高效液相色谱法同时测定不同产地20批（编号S1～S20）土茯苓中5-

-咖啡酰基莽草酸、新落新妇苷、落新妇苷、新异落新妇苷、异落新妇苷、黄杞苷、白藜芦醇、异黄杞苷的含量。采用化学计量学方法对20批土茯苓样品进行评价，并筛选影响土茯苓质量的差异标志物。

结果

所测8个成分在各自检测质量浓度范围内线性关系良好（

≥0.999 6）；精密度、重复性和稳定性（24 h）试验的RSD均小于2.00%（

＝6）；平均加样回收率为97.60%～106.40%，RSD均小于2.00%（

＝6）。聚类分析结果显示，浙江产（S1～S5）和江西产（S6～S10）样品聚为一类，湖南产（S11～S15）样品聚为一类，云南产（S16～S20）样品聚为一类；主成分分析结果显示，前2个主成分可反映土茯苓中8个成分85.60%的信息量；偏最小二乘-判别分析结果显示，5-

-咖啡酰基莽草酸、落新妇苷、异落新妇苷和新落新妇苷的变量重要性投影值均大于1。

结论

不同产地土茯苓中上述8个成分含量存在差异，5-

-咖啡酰基莽草酸、落新妇苷、异落新妇苷和新落新妇苷可能是影响土茯苓质量的差异标志物。

Abstract

OBJECTIVE

To provide reference for

Smilax glabra

quality control.

METHODS

UPLC method was established to simultaneously determine the contents of 5-

-caffeoylshikimic acid， neoastilbin， astilbin， neoisoastilbin， isoastilbin， engelitin， resveratrol and isoengelitin in 20 batches of

S. glabra

from different areas （No. S1-S20）. The quality evaluation of 20 batches of samples was performed by chemometrics； the differential biomarkers that affected the quality of

S. glabra

were screened.

RESULTS

The measured 8 components had good linear relationship within the range of measured concentration （

≥0.999 6）. RSDs of precision， repeatability and stability tests （24 h） were all lower

than 2.00% （

＝6）. The average recoveries varied between 97.60% and 106.40% （RSDs were all lower than 2.00%，

＝6）. Cluster analysis showed that the samples produced in Zhejiang （S1-S5） and Jiangxi （S6-S10） were clustered into one category； the samples produced in Hunan （S11-S15） were clustered into one category； the samples produced in Yunnan （S16-S20） were clustered into one category. Principal component analysis showed that the first two principal components could represent 85.60% information of 8 components in

S. glabra

. Partial least squares-discriminant analysis showed that variable importance projection values of 5-

-caffeoylshikimic acid， astilbin， isoastilbin and neoastilbin were all greater than 1.

CONCLUSIONS

There are differences in the contents of the above 8 components in

S. glabra

from different origins； 5-

-caffeoylshikimic acid， astilbin， isoastilbin and neoastilbin may be differential markers affecting the quality of

S. glabra

关键词

土茯苓化学计量学含量测定超高效液相色谱法5-O-咖啡酰基莽草酸落新妇苷异落新妇苷新落新妇苷

Keywords

chemometricscontent determinationUPLC5-O-caffeoylshikimic acidastilbinisoastilbinneoastilbin

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⁰

Content determination and chemometric analysis of 8 components in Smilax glabra

DOI：10.6039/j.issn.1001-0408.2024.19.10

摘要

Abstract

关键词

Keywords

references