Effects and mechanism of persimmon leaf extract on IEC-6 cell ferroptosis induced by H<sub>2</sub>O<sub>2</sub>

ZHANG Xuexia; ZHOU Min; ZHOU Hongyan; WANG Lifei; LI Huani; LIU Changhe; XU Hongde; ZHANG Mingli

doi:10.6039/j.issn.1001-0408.2025.01.11

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Effects and mechanism of persimmon leaf extract on IEC-6 cell ferroptosis induced by H₂O₂

更新时间：2025-09-25

- Effects and mechanism of persimmon leaf extract on IEC-6 cell ferroptosis induced by H₂O₂
- ZHONGGUO YAOFANG Vol. 36, Issue 1, Pages: 64-70(2025)
- 作者机构：
  
  1.河南省中西医结合医院中药研究所，郑州　450004
  2.郑州大学药学院，郑州　450001
  3.河南中西医结合医院检验科，郑州　450004
  4.郑州大学第二附属医院肾病科，郑州　450014
  5.河南省中西医结合医院肺病科，郑州　450004
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2025.01.11
  CLC： R965
- Received：19 June 2024，
  
  Revised：2024-11-08，
  
  Accepted：11 November 2024，
  
  Published：15 January 2025
- 稿件说明：
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张雪侠,周敏,周红艳,等.柿叶提取物对H₂O₂诱导IEC-6细胞铁死亡的影响及机制研究[J].中国药房,2025,36(01):64-70.

ZHANG Xuexia,ZHOU Min,ZHOU Hongyan,et al.Effects and mechanism of persimmon leaf extract on IEC-6 cell ferroptosis induced by H₂O₂[J].ZHONGGUO YAOFANG,2025,36(01):64-70.
张雪侠,周敏,周红艳,等.柿叶提取物对H₂O₂诱导IEC-6细胞铁死亡的影响及机制研究[J].中国药房,2025,36(01):64-70. DOI： 10.6039/j.issn.1001-0408.2025.01.11.

ZHANG Xuexia,ZHOU Min,ZHOU Hongyan,et al.Effects and mechanism of persimmon leaf extract on IEC-6 cell ferroptosis induced by H₂O₂[J].ZHONGGUO YAOFANG,2025,36(01):64-70. DOI： 10.6039/j.issn.1001-0408.2025.01.11.

摘要

目的

探讨柿叶（PL）提取物对过氧化氢（H

）诱导IEC-6细胞铁死亡的影响及潜在机制。

方法

以IEC-6细胞为对象，考察铁死亡抑制剂ferrostatin-1对H

诱导IEC-6细胞活力的影响；将IEC-6细胞分为对照组、H

组、H

+PL 25 μg/mL组、H

+PL 50 μg/mL组，检测其氧化应激指标[丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性和活性氧（ROS）水平

]

、线粒体膜电位（MMP）以及核转录因子红系2相关因子2（Nrf2）、血红素氧合酶1（HO-1）、还原型烟酰胺腺嘌呤二核苷酸磷酸/醌氧化还原酶1（NQO-1）、胱氨酸/谷氨酸反向转运体（xCT）、谷胱甘肽过氧化物酶4（GPX4）、铁蛋白重链（FTH）mRNA及蛋白的表达情况。

结果

铁死亡抑制剂ferrostatin-1可显著升高H

诱导细胞的存活率（

＜0.01）。与对照组比较，H

组细胞MDA含量和ROS水平，Nrf2、NQO-

1 mRNA及Nrf2、HO-1蛋白的表达均显著升高或上调；SOD活性和MMP，xCT、GPX4、FTH mRNA及GPX4、FTH蛋白的表达均显著降低或下调（

＜0.01或

＜0.05）。与H

组比较，H

+PL 25、50 μg/mL组细胞的氧化应激指标均有不同程度回调，MMP均显著升高，Nrf2、HO-1、NQO-1，xCT、GPX4、FTH mRNA及蛋白的表达均有不同程度上调，部分指标组间比较差异有统计学意义（

＜0.01或

＜0.05）。

结论

PL提取物能减轻H

所导致的细胞线粒体膜损伤、ROS异常堆积，上述作用可能与激活Nrf2/HO-1信号通路从而抑制铁死亡有关。

Abstract

OBJECTIVE

To investigate the effects and potential mechanism of persimmon leaf （PL） extract against ferroptosis induced by H

in IEC-6 cells.

METHODS

Using IEC-6 cells as object， the effects of ferroptosis inhibitor ferrostatin-1 on IEC-6 cell viability induced by H

were investigated； IEC-6 cells were divided into control group， H

group， H

+PL 25 μg/mL group and H

+PL 50 μg/mL group. The levels of oxidant stress indexes [content of malondialdehyde （MDA）， activity of superoxide dismutase （SOD）， and levels of reactive oxygen species （ROS）

]

， mitochondrial membrane potential （MMP） as well as mRNA and protein expressions of nuclear factor-erythroid-2 related factor 2 （Nrf2）， heme oxygenase-1 （HO-1）， NADPH/quinone oxidoreductase-1 （NQO-1）， cystine/glutamate anti-porter （xCT）， glutathione peroxidase 4 （GPX4） and ferritin heavy chain （FTH） were detected.

RESULTS

Ferroptosis inhibitor ferrostatin-1 could significantly increase the survival rate of H

-induced cells （

＜0.01）. Compared with the control group， MDA content， ROS level， mRNA expressions of Nrf2 and NQO-1 as well as protein expressions of Nrf2 and HO-1 were increased or up-regulated significantly， while SOD activity， MMP， mRNA expressions of xCT， GPX4 and FTH as well as protein expression

s of GPX4 and FTH were decreased or down-regulated significantly （

＜0.01 or

＜0.05）. Compared with the H

group， oxidative stress indexes of H

+PL 25， 50 μg/mL groups were reversed to different extents， MMP level was increased significantly， as well as mRNA and protein expressions of Nrf2， HO-1， NQO-1， xCT， GPX4 and FTH were up-regulated to different extents； there were statistical significances in some indexes between groups （

＜0.01 or

＜0.05）.

CONCLUSIONS

PL extract can alleviate mitochondrial membrane damage and abnormal accumulation of ROS caused by H

， which may be related to the inhibition of ferroptosis by activating the Nrf2/HO-1 signaling pathway.

关键词

Keywords

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Effects and mechanism of persimmon leaf extract on IEC-6 cell ferroptosis induced by H2O2

DOI：10.6039/j.issn.1001-0408.2025.01.11

摘要

Abstract

关键词

Keywords

references

Effects and mechanism of persimmon leaf extract on IEC-6 cell ferroptosis induced by H₂O₂