OBJECTIVE

2

To establish the fingerprint of

Gerbera delavayi

and the methods for the content determination of 11 components in

G. delavayi

.

METHODS

2

High-performance liquid chromatography（HPLC）was adopted to establish the fingerprints of 13 batches of

G. delavayi

（No.S1-S13）， and the similarities were evaluated according to

Similarity Evaluation System of Chromatographic Fingerprint of TCM

（

2012 edition

）， while the common peaks were identified. Hierarchical clustering analysis （HCA）， principal component analysis （PCA） and orthogonal partial least square-discriminant analysis （OPLS-DA） were carried out by using SPSS 25.0 software and SIMCA 14.1 software. The contents of neochlorogenic acid， chlorogenic acid， cryptochlorogenic acid， 3，8-dihydroxy-4-methoxy-2-oxo-2

H

-1-benzopyran-5-carboxylic acid， caffeic acid， 3-hydroxy-4-methoxy-2-oxo-2

H

-1-benzopyran- 5-carboxylic acid， luteolin-7-

O

-

β

-D-glucoside， isochlorogenic acid A， apigenin-7-

O

-

β

-D-glucoside， isochlorogenic acid C and xanthotoxin were determined by HPLC.

RESULTS

2

The similarities in HPLC fingerprint of 13 batches of

G. delavayi

w

ere 0.801-0.994； a total of 38 common peaks were identified and 13 common peaks were identified. The results of HCA showed that S1-S5 and S7 were clustered into one group， S6 into one category， S8 into one category， S9 and S11 into one category， S10， S12 and S13 into one category， and the results of PCA were consistent with them. The results of OPLS-DA showed that variable importance values for the projection of peak 7 （chlorogenic acid）， peak 21 （isochlorogenic acid A）， peak 26 （xanthotoxin）， peak 19 （isochlorogenic acid B）， peak 33， peak 13， peak 23 （isochlorogenic acid C）， peak 2 （new chlorogenic acid）， peak 17 （luteolin-7-

O

-

β

-D-glucoside） were greater than 1. The above 11 components had good linearity in their respective detection concentration ranges （

r

was greater than 0.999）. RSDs of precision， repeatability， and stability tests were not more than 2% （

n

＝6）. The average recovery rates were 92.54%-105.55%， and the RSDs were 0.83%-1.93% （

n

＝6）. The average contents of 11 components were 0.744， 5.014， 0.646， 0.431， 0.069， 0.582， 0.979， 2.754， 0.157， 1.284 and 2.943 mg/g， respectively.

CONCLUSIONS

2

The constructed HPLC fingerprint and content determination methods are simple， accurate and stable， which can provide reference for quality control of

G. delavayi

. Xanthotoxin， chlorogenic acid， isochlorogenic acid A， luteolin-7-

O

-

β

-D-glucoside， isochlorogenic acid C and new chlorogenic acid can be used as markers for

G. delavayi

.