OBJECTIVE

2

To determine the contents of 15 components in Aurantii Fructus Immaturus from different origins （

Citrus aurantium

，

C. junos

，

C. aurantium

Linn.，

C. sinensis

Osb

.

，

C. sinensis

）， screen differential components， and provide references for the quality evaluation of Aurantii Fructus Immaturus.

METHODS

2

HPLC method was adopted to determine the contents of synephrine，

N

-methyltyramine， 5，7-dihydroxychromone-7-neohesperidoside， neoponcirin， narirutin， naringin， hesperidin， neohesperidin， naringenin， hesperetin， sinensetin， nobiletin， tangeretin， 5-demethylnobiletin， and auraptene in 46 batches of Aurantii Fructus Immaturus from different origins. The determination was performed on Waters Symmetry C

18

column with mobile phase consisted of acetonitrile-0.1% formic acid （gradient elution） at the flow rate of 1.0 mL/min； column temperature was set at 40 ℃， detection wavelength was 284 nm， and sample injection volume was 5 μL. The differences between different origins of Aurantii Fructus Immaturus were analyzed by cluster analysis， principal component analysis （PCA） and orthogonal partial l

east squares-discriminant analysis （OPLS-DA）， and differential components were screened.

RESULTS

2

The linear relationships of the aforementioned 15 components were all good within the tested mass concentration ranges （all

r

＞0.999 0）. The RSDs for precision， stability （24 h）， and repeatability tests were all less than 2.00%. The average recovery rate ranged from 91.1% to 103.9% （all RSDs＜3.10%）. Cluster analysis， PCA， and OPLS-DA revealed that

C. sinensis

Osb. and

C. sinensis

were clustered into one category， while

C. aurantium

，

C. junos

and

C. aurantium

Linn. were clustered into another category. The variable importance projection values for neohesperidin， auraptene， naringin， neoponcirin， tangeretin， hesperidin， sinensetin， and 5，7-dihydroxychromone-7-neohesperidoside were all greater than 1.

CONCLUSIONS

2

In this study， the contents of 15 components in Aurantii Fructus Immaturus from different origins are determined， and 8 differential components， including neohesperidin， auraptene， naringin， and neoponcirin， are screened out.