Effects of astragaloside Ⅳ on arterial endothelial tissue damage in rats with intracranial aneurysm

CAI Qiang; LIU Liuqing; TANG Jiayu

doi:10.6039/j.issn.1001-0408.2025.13.11

您当前的位置：

首页 >

文章列表页 >

Effects of astragaloside Ⅳ on arterial endothelial tissue damage in rats with intracranial aneurysm

更新时间：2025-09-24

- Effects of astragaloside Ⅳ on arterial endothelial tissue damage in rats with intracranial aneurysm
- ZHONGGUO YAOFANG Vol. 36, Issue 13, Pages: 1617-1621(2025)
- 作者机构：
  
  1.湖南省第二人民医院介入医学中心，长沙　417000
  2.湖南省第二人民医院神经内科，长沙　417000
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2025.13.11
  CLC： R965;R285
- Received：14 March 2025，
  
  Revised：2025-05-31，
  
  Accepted：03 June 2025，
  
  Published：15 July 2025
- 稿件说明：
移动端阅览
蔡强,刘柳青,唐佳宇.黄芪甲苷对颅内动脉瘤大鼠动脉血管内皮组织损伤的影响[J].中国药房,2025,36(13):1617-1621.

CAI Qiang,LIU Liuqing,TANG Jiayu.Effects of astragaloside Ⅳ on arterial endothelial tissue damage in rats with intracranial aneurysm[J].ZHONGGUO YAOFANG,2025,36(13):1617-1621.
蔡强,刘柳青,唐佳宇.黄芪甲苷对颅内动脉瘤大鼠动脉血管内皮组织损伤的影响[J].中国药房,2025,36(13):1617-1621. DOI： 10.6039/j.issn.1001-0408.2025.13.11.

CAI Qiang,LIU Liuqing,TANG Jiayu.Effects of astragaloside Ⅳ on arterial endothelial tissue damage in rats with intracranial aneurysm[J].ZHONGGUO YAOFANG,2025,36(13):1617-1621. DOI： 10.6039/j.issn.1001-0408.2025.13.11.

摘要

目的

研究黄芪甲苷（AST）对颅内动脉瘤（IA）大鼠动脉血管内皮组织损伤的影响，并基于核因子κB（NF-κB）/核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3（NLRP3）信号通路探讨其作用机制。

方法

将大鼠分为假手术组（Sham组，灌胃并腹腔注射等体积生理盐水）、IA组（灌胃并腹腔注射等体积生理盐水）、AST低剂量组（AST-L组，灌胃40 mg/kg AST）、AST高剂量组（AST-H组，灌胃80 mg/kg AST）和AST-H+HY-N2485组（灌胃80 mg/kg的AST并腹腔注射25 mg/kg的NF-κB/NLRP3信号通路激活剂HY-N2485）。每天给药1次，连续8周。末次给药后，检测血清中炎症因子[肿瘤坏死因子α、白细胞介素18（IL-18）、IL-6]、血管内皮生长因子（VEGF）和内皮肽（ET）水平，观察IA形态，检测血管组织中血管性血友病因子（vWF）、血管细胞黏附分子1（VCAM-1）、内皮型一氧化氮合酶（eNOS）和NF-κB/NLRP3信号通路相关蛋白表达水平。

结果

与Sham组比较，IA组大鼠颅底动脉环有明显突起，动脉血管内皮细胞有明显损伤；血清中炎症因子和VEGF、ET水平，血管组织中vWF、VCAM-1、NLRP3蛋白表达水平以及NF-κB蛋白磷酸化水平均显著升高（

＜0.05），动脉瘤、内部弹性层破裂均显著增大（

＜0.05），eNOS蛋白表达水平显著降低（

＜0.05）；与IA组比较，AST-L组、AST-H组大鼠IA形态及上述指标水平均显著改善（

＜0.05），且AST-H组较AST-L组改善得更明显（

＜0.05）；HY-N2485可显著减轻AST对IA大鼠血管内皮组织损伤的改善作用（

＜0.05）。

结论

AST可能通过抑制NF-κB/NLRP3信号通路来抑制炎症因子表达，减轻炎症和血管内皮组织损伤，从而抑制IA形成。

Abstract

OBJECTIVE

To investigate the effect of astragaloside Ⅳ（AST） on the injury of arterial endothelial tissue in rats with intracranial aneurysms （IA）， and to explore its mechanism of action based on the nuclear factor κB （NF-κB）/nucleotide-binding domain leucine-rich repeat and pyrin domain-containing protein 3 （NLRP3） signaling pathway.

METHODS

Rats were divided into Sham group （intragastric administration and intraperitoneal injection of the same volume of normal saline）， IA group （intragastric administration and intraperitoneal injection of the same volume of normal saline）， AST low-dose group （AST-L group， intragastric administration of 40 mg/kg AST）， AST high-dose group （AST-H group， intragastric administration of 80 mg/kg AST）， AST-H+HY-N2485 group [intragastric administration of 80 mg/kg AST and intraperitoneal injection of 25 mg/kg HY-N2485 （activator of NF-κB/NLRP3 signaling pathway）]. They were given relevant medicine， once a day， for 8 consecutive weeks. After last medication， the levels of inflammatory factors [serum tumor necrosis factor-α （TNF-α）， interleukin-18 （IL-18）， IL-6] and vascular endothelial growth factor （VEGF） and endothelin （ET） were detected； the morphology of IA was observed； the expressions of von Willebrand factor （vWF）， vascular cell adhesion molecule-1 （VCAM-1）， endothelial nitric oxide synthase （eNOS）， and NF-κB/NLRP3 pathway related proteins in vascular tissue were also determined.

RESULTS

Compared with the Sham group， the basilar arterial ring of rats in the IA group had obvious protrusions， and the arterial vascular endothelial cells were significantly damaged. The levels of inflammatory factors， VEGF and ET in serum， as well as the expression levels of vWF， VCAM-1 and NLRP3 proteins and the phosphorylation level of NF-κB protein in vascular tissues were increased significantly （

＜0.05）. Aneurysms and ruptures of the internal elastic layer were significantly increased （

＜0.05）， while the expression level of eNOS protein was significantly decreased （

＜0.05）. Compared with IA group， the morphology of IA and the levels of above indexes were all improved signi

ficantly in AST-L and AST-H groups （

＜0.05），and the improvement in the AST-H group was more significant than that in the AST-L group （

＜0.05）； HY-N2485 could attenuate the improvement effect of AST on vascular endothelial tissue damage in IA rats （

＜0.05）.

CONCLUSIONS

AST may inhibit the expression of inflammatory factors， alleviate inflammation and vascular endothelial tissue damage in IA rats by inhibiting NF-κB/NLRP3 signaling pathway， thereby inhibiting the formation of IA.

关键词

Keywords

references

NAFEES AHMED S ， PRAKASAM P . A systematic review on intracranial aneurysm and hemorrhage detection using machine learning and deep learning techniques [J ] . Prog Biophys Mol Biol ， 2023 ， 183 ： 1 - 16 .

STRANGE F ， GRUTER B E ， FANDINO J ， et al . Preclinical intracranial aneurysm models：a systematic review [J ] . Brain Sci ， 2020 ， 10 （ 3 ）： 134 .

HUANG J X ， ZHANG H ， YOU L T ， et al . Coenzyme Q10 inhibits intracranial aneurysm formation and progression in a mouse model [J ] . Pediatr Res ， 2022 ， 91 （ 4 ）： 839 - 845 .

李康丽，周奕彤，李艳荣，等 . 黄芪甲苷治疗中枢神经系统疾病作用机制研究进展 [J ] . 中成药， 2023 ， 45 （ 8 ）： 2634 - 2641 .

谭维，傅馨莹，杨仁义，等 . 黄芪甲苷调控Nrf2/HO-1信号通路对血管内皮细胞氧化损伤的影响 [J ] . 湖南中医药大学学报， 2024 ， 44 （ 9 ）： 1592 - 1600 .

孙洋，王洪新 . 黄芪甲苷通过NF-κB/NLRP3信号通路减轻肺动脉高压大鼠的炎症反应 [J ] . 中成药， 2023 ， 45 （ 2 ）： 578 - 582 .

ZHAO Y L ， SHAO C Y ， ZHOU H F ， et al . Salvianolic acid B inhibits atherosclerosis and TNF-α-induced inflammation by regulating NF-κB/NLRP3 signaling pathway [J ] . Phytomedicine ， 2023 ， 119 ： 155002 .

杨成，张燕飞，管宏新，等 . 基于TLR4/NF-κB信号通路上调miR-143对颅内动脉瘤模型大鼠的干预作用 [J ] . 疑难病杂志， 2022 ， 21 （ 8 ）： 850 - 855 .

刘宇，孙媛媛，战爱波，等 . 金丝桃苷对颅内动脉瘤大鼠血管内皮组织Hippo信号通路影响 [J ] . 中国药师， 2022 ， 25 （ 2 ）： 238 - 244 .

YU X J ， WANG Y G ， LU R ， et al . BMP7 ameliorates intervertebral disc degeneration in type 1 diabetic rats by inhibiting pyroptosis of nucleus pulposus cells and NLRP3 inflammasome activity [J ] . Mol Med ， 2023 ， 29 （ 1 ）： 30 .

SUN B ， LIU Z H ， YU Z Q . miRNA-323a-3p promoted intracranial，aneurysm-induced inflammation via AMPK/NF-κB signaling pathway by AdipoR1 [J ] . Adv Clin Exp Med ， 2022 ， 31 （ 11 ）： 1243 - 1254 .

YANG S Z ， LIU Q Y ， YANG J H ， et al . Increased levels of serum IL-15 and TNF-β indicate the progression of human intracranial aneurysm [J ] . Front Aging Neurosci ， 2022 ， 14 ： 903619 .

SUN J W ， ZHANG L F ， CHENG Q J ， et al . Aberrant expression and regulatory role of histone deacetylase 9 in vascular endothelial cell injury in intracranial aneurysm [J ] . Biomol Biomed ， 2024 ， 24 （ 1 ）： 61 - 72 .

LIU Y ， SU Y X ， CHEN L ， et al . Exploring the roles and therapeutic implications of melatonin-mediated KLF6 in the development of intracranial aneurysm [J ] . Ann Med ， 2024 ， 56 （ 1 ）： 2397568 .

USATEGUI-MARTÍN R ， JIMÉNEZ-ARRIBAS P ， SAKAS-GANDULLO C ， et al . Endothelial nitric oxide synthase rs1799983 gene polymorphism is associated with the risk of developing intracranial aneurysm [J ] . Acta Neurochir （Wien）， 2023 ， 165 （ 5 ）： 1261 - 1267 .

SU H ， LIU B ， CHEN H M ， et al . LncRNA ANRIL me- diates endothelial dysfunction through BDNF downregulation in chronic kidney disease [J ] . Cell Death Dis ， 2022 ， 13 （ 7 ）： 661 .

ZHANG P Y ， WU P F ， KHAN U Z ， et al . Exosomes derived from LPS-preconditioned bone marrow-derived MSC modulate macrophage plasticity to promote allograft survival via the NF-κB/NLRP3 signaling pathway [J ] . J Nanobiotechnology ， 2023 ， 21 （ 1 ）： 332 .

YU H M ， LIU Q Y ， XIE M H ， et al . Nesfatin-1 inhibits cerebral aneurysms by activating Nrf2 and inhibiting NF-κB signaling [J ] . CNS Neurosci Ther ， 2024 ， 30 （ 8 ）： e14864 .

JIANG Z X ， HUANG J X ， YOU L T ， et al . Pharmacological inhibition of STAT3 by BP-1-102 inhibits intracranial aneurysm formation and rupture in mice through modula- ting inflammatory response [J ] . Pharmacol Res Perspect ， 2021 ， 9 （ 1 ）： e00704 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Portulaca oleracea cream on skin pruritus and barrier function in allergic contact dermatitis mice

Clinical observation of ivabradine in the treatment of chronic heart failure in end-stage renal disease patients undergoing maintenance hemodialysis

Research progress on traditional Chinese medicine improving polycystic ovary syndrome by regulating NF-κB signaling pathway

Improvement effects of vitexin on asthmatic model mice based on Notch signaling pathway

Effect and mechanism of cucurbitacin B preventing sepsis-induced acute lung injury in mice

Related Author

LUO Jiangli

WU Nana

TANG Ying

KONG Xiangyan

FAN Guanwei

PU Xiang

CHEN Xia

WANG Xiaoxue

Related Institution

School of Pharmacy， Guizhou University of Traditional Chinese Medicine

Experimental Center， the First Affiliated Hospital of Tianjin University of Chinese Medicine

Dept. of Dermatology， the First Affiliated Hospital of Guizhou University of Chinese Medicine

Dept. of Cardiology， Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital

Endoscopy Room， Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital

Address：8th Floor, Sihuan Building, No. 129 Daping Main Street, Yuzhong District, Chongqing, China Postal code：400042
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰