OBJECTIVE

2

To explore the influence of

Zingiber officinale

and processed

Z. officinale

on pharmacodynamic indexes and intestinal flora on gastric ulcer rats with spleen-stomach deficiency and cold type before and after processing with sand.

METHODS

2

The SD rats were randomly divided into normal group， model group， positive control group （Compound tianqi weitong capsule 0.45 g/kg），

Z. officinale

high-dose （15.0 g/kg） and low-dose （7.5 g/kg） groups， processed

Z. officinale

high-dose （15.0 g/kg） and low-dose （7.5 g/kg） groups， with 10 rats in each group. The rat model of gastric ulcer with spleen-stomach deficiency and cold type was established by intragastric administration of vinegar （day 1-10） and absolute ethanol （day 11）. Administration groups were given relevant liquid intragastrically， and normal group and model group were given water intragastrically （day 5-10）. One hour after intragastric administration of absolute ethanol， blood was taken from the femoral artery of rat

s， the serum contents of motilin （MTL）， gastrin （GAS），epidermal growth factor （EGF） as well as 4 items of blood coagulation [activated partial thromboplastin time （APTT）， prothrombin time （PT）， thrombin time （TT）， fibrinogen （FIB）

]

were detect. The ulcer index and inhibition rate of ulcer in gastric tissue were calculated. The pathological changes of gastric tissue were observed， and the number and area of erosions were recorded. The diversity of gut microbiota in fecal samples of rats was detected.

RESULTS

2

Compared with model group，the contents of MTL （except for processed

Z. officinale

low-dose group），GAS （except for processed

Z. officinale

low-dose group），EGF （except for

Z. officinale

groups） and FIB （except for

Z. officinale

groups）， inhibitory rate of ulcer （only positive control group） were all increased significantly （

P

＜0.05）. APTT （except for

Z. officinale

groups），PT （only processed

Z. officinale

high-dose group），TT （except for

Z. officinale

groups）， ulcer index （except for

Z. officinale

groups）， the number （except for

Z. officinale

groups） and area of erosions （except for

Z. officinale

groups） were shortened and decreased significantly （

P

＜0.05）； improvement effects of processed

Z. officinale

were better than those of the same dose of

Z. officinale

on EGF， 4 items of blood coagulation （except for PT， TT， FIB of processed

Z. officinale

low-dose group）， ulcer index （except for processed

Z. officinale

low-dose group） and inhibitory rate of ulcer （

P

＜0.05）. Compared with model group，α diversity indexes as ACE， Shannon and Simpson of intestinal microorganisms in rats were increased significantly in processed

Z. officinale

group； the relative abundance of Proteobacteria was decreas

ed significantly in processed

Z. officinale

group， while that of Bacteroidetes was increased significantly （

P

＜0.05）； the relative abundance of

Limosilactobacillus

was decreased significantly in

Z. officinale

group （

P

＜0.05）.

CONCLUSIONS

2

Z. officinale

and processed

Z. officinale

can improve the symptoms of spleen-stomach deficiency and cold，and enhance gastrointestinal function by increasing the content of GAS and MTL. Processed

Z. officinale

can significantly inhibit gastric ulcer of spleen-stomach deficiency and cold type， which is related to the promotion of mucosal protection and repair，improvement of coagulation functionand adjustment of gut microbiotadisorder.