OBJECTIVE

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To establish a method for simultaneously determining the contents of camphor， menthol， borneol and methyl salicylate in Compound shexiang xuelian liuzhi plaster.

METHODS

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The test solution was prepared by reflux extraction with ethyl acetate， and was determined by gas chromatography （GC）. A Shimadzu SH-Rtx-Wax capillary column was used as the chromatographic column， and a flame ionization detector was used as the detector. The detector temperature and the injector temperature were both set at 200 ℃. The flow rate of carrier gas （nitrogen） was 2.0 mL/min， the separation ratio was 20∶1， and the sample size was 1.0 μL.

RESULTS

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The linear ranges of camphor， menthol， borneol （calculated by t

he sum of isoborneol and borneol） and methyl salicylate were 11.5-230.4， 10.6-211.6， 11.3-225.5， 11.0-219.1 μg/mL （

r

＞0.999）. RSDs of the precision， repeatability and stability （48 h） tests were all less than 4%. The average recoveries of the four components were 100.7%， 99.7%， 98.9% and 100.7% （RSDs were 4.3%， 2.9%， 2.2%， 3.7%，

n

＝9）. The contents of camphor， menthol， borneol and methyl salicylate in two specifications of Compound shexiang xuelian liuzhi plaster were 16.8-19.5， 4.6-6.0， 9.8-11.9， 6.9-8.2 mg/piece （7 cm×10 cm/piece）， and 8.3-8.6， 2.2-2.4， 4.7-4.8， 3.2-3.6 mg/piece （5 cm×7 cm/piece）.

CONCLUSIONS

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The method is successfully established for simultaneous determination of four volatile components in Compound shexiang xuelian liuzhi plaster.