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Effects of ligustilide on chemotherapy resistance of cervical cancer cells
- ZHONGGUO YAOFANG Vol. 35, Issue 13, Pages: 1582-1587(2024)
- 作者机构：
1.庆阳市中医医院妇产科，甘肃 庆阳 745000
2.西北妇女儿童医院妇三科，西安 710061
3.西安医学院基础医学部遗传教研室，西安 710061
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2024.13.07
CLC： R965Published：15 July 2024，
Received：05 December 2023，
Revised：17 May 2024，
- 稿件说明：
扫 描 看 全 文
张文渊,王倩,张丽红等.藁本内酯对宫颈癌细胞化疗耐药性的影响 Δ[J].中国药房,2024,35(13):1582-1587.
ZHANG Wenyuan,WANG Qian,ZHANG Lihong,et al.Effects of ligustilide on chemotherapy resistance of cervical cancer cells[J].ZHONGGUO YAOFANG,2024,35(13):1582-1587.
张文渊,王倩,张丽红等.藁本内酯对宫颈癌细胞化疗耐药性的影响 Δ[J].中国药房,2024,35(13):1582-1587. DOI： 10.6039/j.issn.1001-0408.2024.13.07.
ZHANG Wenyuan,WANG Qian,ZHANG Lihong,et al.Effects of ligustilide on chemotherapy resistance of cervical cancer cells[J].ZHONGGUO YAOFANG,2024,35(13):1582-1587. DOI： 10.6039/j.issn.1001-0408.2024.13.07.
摘要
目的
2
基于Hippo-Yes相关蛋白（YAP）信号通路探讨藁本内酯对宫颈癌细胞化疗耐药性的影响。
方法
2
将人宫颈癌顺铂耐药细胞HeLa/DDP分为对照组、顺铂组（10 μmol/L顺铂）和顺铂+低、中、高浓度藁本内酯组（10 μmol/L顺铂+25、50、100 μmol/L藁本内酯），检测各组细胞的增殖、凋亡、迁移、侵袭情况，以及YAP、具有PDZ结合基序的转录共激活因子（TAZ）mRNA和YAP、TAZ、基质金属蛋白酶2（MMP2）、Ki67、切割型胱天蛋白酶3（cleaved-caspase-3）、caspase-3蛋白的表达情况。
结果
2
与对照组比较，顺铂组细胞的增殖抑制率、凋亡率和cleaved-caspase-3/caspase-3均显著升高；划痕愈合率、侵袭细胞数，YAP、TAZ mRNA和YAP、TAZ、MMP2、Ki67蛋白的表达水平均显著降低（
P
＜0.05）。与顺铂组比较，顺铂+低、中、高浓度藁本内酯组细胞的增殖抑制率、凋亡率和cleaved-caspase-3/caspase-3均进一步升高；划痕愈合率、侵袭细胞数，YAP、TAZ mRNA和YAP、TAZ、MMP2、Ki67蛋白的表达水平均进一步降低，且呈剂量依赖性（
P
＜0.05）。
结论
2
藁本内酯可通过抑制Hippo-YAP信号通路提高宫颈癌耐药细胞对顺铂的敏感性。
Abstract
OBJECTIVE
2
To investigate the effects of ligustilide on chemotherapy resistance of cervical cancer cells based on Hippo-Yes-associated protein （YAP） signaling pathway.
METHODS
2
Human cervical cancer cisplatin-resistant cells HeLa/DDP were divided into control group， cisplatin group （10 μmol/L cisplatin）， cisplatin+ligustilide low-， medium- and high-concentration groups （10 μmol/L cisplatin+25， 50， 100 μmol/L ligustilide）. The proliferation， apoptosis， migration and invasion of HeLa/DDP cells were all detected in each group. The mRNA expressions of YAP and transcriptional coactivator with PDZ binding motif （TAZ） as well as the protein expressions of YAP， TAZ， matrix metalloproteinase 2 （MMP2）， Ki67， cleaved-caspase-3 and caspase-3 were determined in HeLa/DDP cells.
RESULTS
2
Compared with control group， the inhibitory rate， apoptotic rate and cleaved-caspase-3/caspase-3 of cisplatin group were increased significantly； scratch healing rate， the number of invasive cells， the mRNA expressions of YAP and TAZ， and the protein expressions of YAP， TAZ， MMP2 and Ki67 were decreased significantly in cisplatin group （
P
＜0.05）. Compared with cisplatin group， the inhibitory rate of cell proliferation， apoptotic rate and cleaved-caspase-3/caspase-3 were further increased in cisplatin+ligustilide low-， medium- and high-concentration groups， while scratch healing rate， the number of inva
sive cells， the mRNA expressions of YAP and TAZ， and the protein expressions of YAP， TAZ， MMP2 and Ki67 were further decreased， in a dose-dependent manner （
P
＜0.05）.
CONCLUSIONS
2
Ligustilide can increase the sensitivity of drug-resistant cervical cancer cells to cisplatin by inhibiting Hippo-YAP signaling pathway.
关键词
藁本内酯宫颈癌细胞顺铂化疗耐药性Hippo-YAP信号通路
Keywords
cervical cancer cellscisplatinchemotherapy resistanceHippo-YAP signaling pathway
references
TIAN J，CHENG L，KONG E Q，et al. linc00958/miR-185-5p/RSF-1 modulates cisplatin resistance and angiogenesis through AKT1/GSK3β/VEGFA pathway in cervical cancer[J]. Reprod Biol Endocrinol，2022，20（1）：132.
SHEN N N，LIN J H，LIU P P. EBF1 promotes the sensitivity of cervical cancer cells to cisplatin via activating FBN1 transcription[J]. Mol Biol （Mosk），2023，57（3）：503-504.
PENG D，WANG Y X，HUANG T H，et al. Ligustilide improves cognitive impairment via regulating the SIRT1/IRE1α/XBP1s/CHOP pathway in vascular dementia rats[J]. Oxid Med Cell Longev，2022，2022：6664990.
GENG P Y，ZHAO J H，LI Q，et al. Z-ligustilide combined with cisplatin reduces PLPP1-mediated phospholipid synthesis to impair cisplatin resistance in lung cancer[J]. Int J Mol Sci，2023，24（23）：17046.
MAEHAMA T，NISHIO M，OTANI J，et al. The role of Hippo-YAP signaling in squamous cell carcinomas[J]. Cancer Sci，2021，112（1）：51-60.
MOYA I M，HALDER G. Hippo-YAP/TAZ signaling in organ regeneration and regenerative medicine[J]. Nat Rev Mol Cell Biol，2019，20（4）：211-226.
KIM H B，MYUNG S J. Clinical implications of the Hippo-YAP pathway in multiple cancer contexts[J]. BMB Rep，2018，51（3）：119-125.
YANG F，LI L，ZHANG J H，et al. TRIP6 accelerates the proliferation and invasion of cervical cancer by upregula- ting oncogenic YAP signaling[J]. Exp Cell Res，2020，396（1）：112248.
黄根华，谭布珍，占伏良，等. 基于Hippo/yap/TAZ途径探讨雷公藤内酯醇-聚乙烯亚胺-环糊精逆转卵巢癌耐药的作用及机制[J]. 中药新药与临床药理，2020，31（2）：185-191.
HUANG G H，TAN B Z，ZHAN F L，et al. Effect and mechanism of Triptolide-polyethleneimine-cyclodextrin on reversing drug resistance of ovarian cancer through Hippo/yap/TAZ signaling pathway[J]. Tradit Chin Drug Res Clin Pharmacol，2020，31（2）：185-191.
申昌梅，廖振蓉，余瑛. 基于PI3K/Akt信号通路探讨槲皮素增强顺铂对人宫颈癌顺铂耐药细胞HeLa/DDP的作用[J]. 中成药，2022，44（11）：3667-3671.
SHEN C M，LIAO Z R，YU Y. Exploring the effect of quercetin on cisplatin-resistant human cervical cancer cell HeLa/DDP based on PI3K/Akt signaling pathway[J]. Chin Tradit Pat Med，2022，44（11）：3667-3671.
QI H Y，JIANG Z Y，WANG C Q，et al. Sensitization of tamoxifen-resistant breast cancer cells by Z-ligustilide through inhibiting autophagy and accumulating DNA damages[J]. Oncotarget，2017，8（17）：29300-29317.
LIN T C，WANG K H，CHUANG K H，et al. Oct-4 induces cisplatin resistance and tumor stem cell-like properties in endometrial carcinoma cells[J]. Taiwan J Obstet Gynecol，2023，62（1）：16-21.
WU E Y，HUANG L P，BAO J H. MiR-96-5p regulates cervical cancer cell resistance to cisplatin by inhibiting lncRNA TRIM52-AS1 and promoting IGF2BP2[J]. Kaohsiung J Med Sci，2022，38（12）：1178-1189.
LIONTOS M，KYRIAZOGLOU A，DIMITRIADIS I，et al. Systemic therapy in cervical cancer：30 years in review[J]. Crit Rev Oncol Hematol，2019，137：9-17.
XIE Q X，ZHANG L L，XIE L，et al. Z-ligustilide：a review of its pharmacokinetics and pharmacology[J]. Phytother Res，2020，34（8）：1966-1991.
YIN L Q，YING L，GUO R，et al. Ligustilide induces apoptosis and reduces proliferation in human bladder cancer cells by NFκB1 and mitochondria pathway[J]. Chem Biol Drug Des，2023，101（6）：1252-1261.
王博，李恩萍，杨长春，等. 汉黄芩素通过调节P53信号通路增强宫颈癌对顺铂的化疗敏感性[J]. 中国免疫学杂志，2020，36（3）：325-331.
WANG B，LI E P，YANG C C，et al. Enhancement on chemosensitivity of cervical carcinoma to cisplatin by wogonin regulating P53 signaling pathway[J]. Chin J Immunol，2020，36（3）：325-331.
周婷婷，沈卫星，陈桐庆，等. 预知子提取物增敏奥沙利铂诱导结肠癌HCT116细胞凋亡的研究[J]. 南京中医药大学学报，2022，38（3）：204-211.
ZHOU T T，SHEN W X，CHEN T Q，et al. Study on Fructus akebiae extraction inducing apoptosis sensitization of colon cancer HCT116 cells to oxaliplatin[J]. J Nanjing Univ Tradit Chin Med，2022，38（3）：204-211.
WANG D Y，HE J X，DONG J X，et al. The HIPPO pathway in gynecological malignancies[J]. Am J Cancer Res，2020，10（2）：610-629.
陈鹏，徐婷，郭丹，等. YAP蛋白在HER2阳性乳腺癌拉帕替尼耐药中的作用[J]. 第三军医大学学报，2020，42（7）：692-698.
CHEN P，XU T，GUO D，et al. Role of yes-associated protein in resistance of HER2+ breast cancer to lapatinib[J]. J Third Mil Med Univ，2020，42（7）：692-698.
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