ABSTRACT OBJECTIVE：To determine the suitable storage conditions，packaging materials and packaging methods of Ziziphi Spinosae Semen，and to provide reference for guaranteeing the quality. METHODS：The same batch of Ziziphi Spinosae Semen samples were packed in plastic woven bag，plastic bag，plastic bag in vacuum，aluminum-plastic composite bag，aluminum-plastic composite bag in vacuum and kraft coated paper bag. They were stored in a cool warehouse（temperature ≤20 ℃，relative humidity was 45％-75％） and drug stability test box [temperature was（40±2）℃，relative humidity was（75±5）％]. Five evaluation indexes were detected during 6-month of long-term cool stability test and accelerated stability test，including characters and the contents of moisture，aflatoxins，jujubosides A and spinosin. RESULTS：The results of 6-month long-term cool stability test showed that the character and the content of moisture，aflatoxins and jujubosides A of Ziziphi Spinosae Semen under different packaging conditions were in line with the requirements of 2015 edition of Chinese Pharmacopeia （part Ⅰ）（shorted for “pharmacopeia”）. The content of spinosin didn’t meet the requirement of pharmacopeia（no less than 0.080％），but the content of spinosin（0.079％）of aluminum-plastic composite bag in vacuum was close to the requirements of pharmacopeia. The results of 6-month accelerated stability test showed that the sample packed in kraft coated paper bag and plastic woven bag were seriously moldy；the sample packed in plastic bag and plastic bag in vacuum were blackened；the color of the sample packed in aluminum-plastic composite bag was slightly darker；but the appearance of the sample packed in aluminum-plastic composite bag in vacuum was basically unchanged. Only when stored for 2-month，the content of aflatoxin B1 in the sample packed in plastic woven bag was 8.64 μg/kg，which exceeded the pharmacopoeia regulation. The moisture content of the sample packed in plastic woven bag and kraft coated paper bag exceeded the highest value （9.0％） required by the pharmacopoeia. Although the content of jujuboside A of samples in each package decreased，it meet the requirements of the pharmacopoeia. Only the content of spinosin （0.084％）of sample packed in plastic bag in vacuum meet the requirements of the pharmacopoeia，and the content of spinosin （0.071％）of sample packed in aluminum-plastic composite bag in vacuum was relatively high. CONCLUSIONS：Ziziphi Spinosae Semen should be stored in a cool and dry place after packed in aluminum-plastic composite bag in vacuum.