OBJECTIVE： To compare the contents of 4 components and toxicity from Miao medicine Chimonanthus nitens samples after drying in the shade ，oven drying ，steam，charring. METHODS ：The contents of scopolin，fraxin，scopoletin and isofraxin were simultaneously determined by HPLC. The separation was performed on Welch-C18 column with mobile phase consisted of acetonitrile-water （gradient elution ）at the flow rate of 1 mL/min. The detection wavelength was set at 214 nm，and column temperature was 30 ℃，and the sample size was 10 μL. Modified Karber method was used to determine the LD 50 and its 95％CI of different processed C. nitens to mice and evaluate its acute toxicity. RESULTS ： The linear range of scopolin ， fraxin， scopoletin and isofraxin were 0.019-1.856，0.016-1.616，0.009-0.920，0.006-0.624 μg（R2 were all not lower than 0.999 5）. RSDs of precision ， stability and reprodu-cibility tests were all lower than 2.0％（n＝6）. The average recoveries were 104.49％，102.22％， 101.45％，99.26％（all RSDs were lower than 2％，n＝6）. The contents of scopolin were 1.119 0％，0.904 3％， 1.068 4％and 0.036 4％；those o f fraxin were 0.867 8％，0.453 9％，0.423 7％and 0.020 5％；those of scopoletin ； those of isofraxidin were 0.110 2％，0.202 1％，0.208 1％ and 0.249 4％in samples after drying in the shade ，oven CX-2018-001） drying， steam， charring. The LD 50 of 4 processedproducts were 4 118.13，3 733.36，1 643.61，＞10 000 qq.com mg/kg samples ；95％CI were （3 748.87，4 523.76）， （3 422.16，4 072.86），（1 520.90，1 776.23），（＞10 000） mg/kg. CONCLUSIONS ：HPLC method is reproducible and precise. It can be used to determine the contents of 4 components in different processed products of C. niten s. The contents of 4 components in different processed products ， and the contents of glycosides toxicity components are decreased significantly. All the 4 processed products were low or non-toxic.