OBJECTIVE：To provide methodological reference for evaluating the r isk of occupational exposure to antineoplastic drugs（ADs）among the medical staff of pharmacy intravenous admixture services （PIVAS）in our hospital . METHODS ：From Aug. to Dec. 2019，15 PIVAS staff were enrolled in the exposure group and other 15 non-PIVAS staff were selected as the control group. The determination of peripheral blood lymphocyte apoptosis ，urine 8-hydroxydeoxyguanosine（8-OHdG）and mutagenicity （Ames）in urine （mutation rates of Salmenella typhimurium TA98 and TA 100 caused by concentrated urine samples of 10，20 and 40 mL were investigated ）were carried out. At the same time ，the physical examination data of 2 groups were collected. Spearman rank analysis was used to analyze the correlation of working years with early apoptosis rate ，late apoptosis rate and total （No.cstc2018jscx-msybX0138） apoptosis rate of peripheral blood lymphocytes as well as urine E-mail：hxgcq1987@126.com 8-OHdG level. The differences of various physical examination indexes between the two groups were compared. For the physical examination indexes with differences，the personnel distribution was counted according to the normal value range. RESULTS ：The median of service length were 3 years in the exposure group and 6 years in control group ，and baseline data of 2 groups were comparable （P＞0.05）. The late apoptosis rate of peripheral blood lymphocytes and urinary 8-OHdG level in the exposure group were significantly higher than control group （P＜ 0.05 or P＜0.01），but there was no statistical significance in early apoptosis rate and total apoptosis rate of peripheral blood lymphocytes between 2 groups（P＞0.05）. The Spearman rank correlation coefficients of service length with the early apoptosis rate，late apoptosis rate ，total apoptosis rate of peripheral blood lymphocytes as well as urine 8-OHdG level were 0.22，0.03，0.19 and 0.44，respectively，which were not statistically significant （P＞0.05）. Compared with control group ，the mutation rates of S. typhimurium TA98 and TA 100 induced by low ，medium and high doses of urine concentrate were increased by 13.33％，20.00％， 26.66％ and 6.67％，13.34％，13.34％ respectively. In health examination data ，blood glucose ，large platelet ratio ，neutrophil ratio，median cell ratio ，platelet count ，mean platelet volume and globulin were significantly different between 2 groups（P＜ 0.05）. However ，most of above difference indexes of 2 groups were within the normal range ，and there was no significant difference in the distribution of abnormal value （P＞0.05）. CONCLUSION ：PIVAS staff in our hospital have certain occupational exposure risk ，but the degree of risk is still within the range of body compensation. It is a feasible method to evaluate the occupational exposure risk of PIVAS staff to ADs by using biological indicators of experimental examination combined with physical examination data.