OBJECTIVE：To study the anti-oxidation and an ti-hypoxia effect of Yuanshen granule on normal mice. METHODS ： Totally 800 mice were randomly divided into blank control group ，Yuanshen granule low-dose ，middle-dose and high-dose groups （10，20，40 g/kg）and Hongjingtian oral liquid group （positive control ，2 mL/kg），with 160 mice in each group. Administration groups were given relevant medicine intragastrically ，once a day ，for consecutive 30 d. Blank control group was given constant volume of normal saline by the same method. At 1st，3rd，7th，14th and 30th day after administration ，8 mice in each group at each time point were randomly selected for anoxia tolerance test under normobaric pressure and decompression ；the contents of MDA and SOD in myocardium and cerebral tissue were measured ；the hemolysis effect of red blood cell induced by H 2O2 was investigated. RESULTS ：Compared with blank control group ，the survival time of anoxia tolerance under normobaric pressure and under decompression （after 7 days of administration ）were prolonged significantly in Yuanshen granule groups （P＜0.05）. The contents of MDA in myocardium and cerebral tissue （after 3 or 7 days of administration ；after 3，7 or 14 days of administration ） were significantly decreased （P＜0.05），and the contents of SOD （after 7，14 or 30 days of administration ；after 7 days of administration）were increased significantly （P＜0.05）；the erythrocyte hemolysis rate （after 3 or 7 days of administration ）was significantly decreased （P＜0.05）. At some time points ，the improvement effect of the above indexes in the Yuanshen granule middle-dose and high-dose groups was significantly better than that of Hongjingtian oral liquid group （P＜0.05）. CONCLUSIONS ： Yuanshen granule can effectively prolong the hypoxia tolerance survival time of normal mice ，it has anti-oxidant and anti-hypoxia effects on normal mice ，and the effect of Yuanshen granule middle-dose and high-dose groups is better than that of Hongjingtian oral liquid group.