OBJECTIVE：To investigate the improvement e ffects of Zingiber officinale decoction （ZOD） on doxorubicin （DOX）-induced mitochondrial function injury of H 9c2 cardiomyocytes. METHODS ：Taking H 9c2 cardiomyocytes as research object，the effects of different concentrations of ZOD （0.125，0.25，0.5，1，2，4，8 mg/mL，by crude drug ，the same below ）on its survival rate were investigated by CCK- 8 assay. The effects of low ，medium and high concentrations of ZOD （0.125，0.25，0.5 mg/mL）on the morphology of H 9c2 cardiomyocytes after DOX （5 μmol/L）induced mitochondrial dysfunction were detected by high content living cell imaging system. The relative number of cells ，the relative fluorescence intensity of living cells and the relative fluorescence intensity of dead cells were analyzed quantitatively. The effects of ZOD （0.5 mg/mL）on related indexes of mitochondrial respiratory function （oxygen consumption rate ，extracellular acidification rate ，baseline oxygen consumption rate ， baseline extracellular acidification rate ，stress oxygen consumption rate and stress extracellular acidification rate ） and energy metabolism（basic respiration level ，maximum respiration level ，ATP production level ，H+ proton leakage level ，spare respiration level and non-mitochondrial respiration level ）were detected by bioenergy analyzer. RESULTS ：After treated with 0.125，0.25，0.5 mg/mL ZOD ，the survival rate of H 9c2 cardiomyocytes were increased significantly （P＜0.01）or had no statistical significance （P＞0.05）. After DOX induced mitochondrial dysfunction of H 9c2 cardiomyocytes，pretreated with 0.125，0.25，0.5 mg/mL（or 0.5 mg/mL）ZOD，the morphology of H 9c2 cardiomyocytes returned to normal and showed regular fibrous adherent distribution. The relative cell number ，fluorescence intensity of living cells ，oxygen consumption rate ，extracellular acidification rate ，baseline oxygen consumption rate ，baseline extracellular acidification rate ，stress oxygen consumption rate ，stress extracellular acidification rate，basic respiration level ，maximal respiration level ，ATP production level ，spare respiration level and non-mitochondrial respiration level were all significantly increased （P＜0.05 or P＜0.01），while relative dead cell fluorescence intensity and H + proton leakage level were significantly decreased （P＜0.01）. CONCLUSIONS ： ZOD can improve the respiratory function and mitochondrial energy metabolism of H 9c2 cardiomyocytes，so as to improve mitochon drial function injury.