OBJECTIVE：To establis h the method for the determination of voriconazole concentration in human plasma. METHODS：After protein precipitation with acetonitrile ，using riluzole as internal standard ，plasma concentration of voriconazole were determined by HPLC method. The determination was performed on Agilent Zorbax Eclipse Plus C 18 column with mobile phase consisted of acetonitrile- 0.01 mol/L monopotassium phosphate solution （38∶62，V/V）at the flow rate of 1.0 mL/min. The column temperature was maintained at 40 ℃，and the detection wavelength was 255 nm. The sample size was 20 μL. Meanwhile，the method was applied to determine plasma concentration of voriconazole in 10 patients receiving voriconazole. RESULTS ：The liner range of voriconazole was 0.2-20.0 μg/mL（r＝0.999 6）. The lower limit of quantitation was 0.2 μg/mL. RSDs of intra-day and inter-day were both lower than 7％（n＝6 or n＝18）. Average accuracies were 98.59％-106.18％. Average extraction recoveries were 86.77％-89.86％（RSD＜4％，n＝6），and the deviations of measured results and theoretical values of stability tests were within ±15％. The plasma concentrations of voriconazole in 10 patients were 0.34-5.04 μg/mL. CONCLUSIONS：The study successfully establish a rapid and efficient HPLC method for the determination of voriconazole in human plasma.