OBJECTIVE： To study the effects of Danhong injection on the pharmacokinetics of atorvastatin calcium （ATV） in rats. METHODS： The plasma concentration of ATV in rats was determined by UPLC-MS/MS using hydrochloric piperidine as internal standard. The determination was performed on AcquityTM BEH C18 column with mobile phase consisted of 5 mmol/L ammonium formate solution （containing 0.1％ formic acid）-methanol （gradient elution）. The ion source was ESI， and the mass transfer ratio （m/z） was 559.20→440.20 （ATV） and 264.20→154.05 （internal standard） by multiple reaction monitoring （MRM） mode.  A total of 24 SD rats were randomly divided into ATV alone group （ATV， 10 mg/kg）， Danhong injection low-dose， medium-dose and high-dose groups （Danhong injection 1， 2， 4 mL/kg+ATV 10 mg/kg）. Blood samples were collected at different time points， and plasma concentration was determined and pharmacokinetic parameters of ATV were calculated. RESULTS： The linear range of ATV was 0.25-62.50 ng/mL， and the limit of quantitation was 0.25 ng/mL. RSDs of precision and stability tests were all lower than 15％. Extraction recovery and matrix effect were all higher than 85％. The pharmacokinetic parameter cmax of ATV in Danhong injection medium-dose and high-dose groups were （23.78±6.4）， （33.66±8.15） ng/mL， which were significantly higher than （13.99±2.54） ng/mL in ATV alone group （P＜0.05）. AUC0-24 h of Danhong injection low-dose， medium-dose and high-dose groups were （67.20±15.22）， （88.16±14.84）， （126.84±31.55） ng·h/mL， which were significantly higher than （46.08±7.47） ng·h/mL in ATV alone group （P＜0.05 or P＜0.01）. CONCLUSIONS： Combined administration of Danhong injection can significantly increase the plasma exposure of ATV in rats.