OBJECTIVE： To establish the quality standard for Glycoprival huayu granules. METHODS： TLC was used for qualitative identification of Hirudo， Atractylodes lancea and Angelica sinensis. HPLC method was adopted to determine the contents of ginsenoside Rg1 and ginsenoside Re. The determination was performed on Phenomenex Luna C18 with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 203 nm，and the column temperature was 25 ℃. The sample size was 10 μL. The content of astilbin was determined by HPLC. The determination was performed on Phenomenex Luna C18 with mobile phase consisted of methanol-0.1％ acetic acid solution （37 ∶ 63，  V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 291 nm，and the column temperature was 25 ℃. The sample size was 10 μL. RESULTS： TLCs of Hirudo， A. lancea and A. sinensis showed clear spots and good separation without interference from negative control. The linear ranges of ginsenoside Rg1， ginsenoside Re and astilbin were 16.3-261.6 μg/mL （r＝0.999 9），5.9-95.2 μg/mL （r＝0.999 8） and 3.2-51.2 μg/mL （r＝0.999 6）. The limits of quantitation were 16.3， 5.9， 3.2 μg/mL； the limits of detection were 2.68， 1.74， 1.09 μg/mL， respectively. RSDs of precision， stability and reproducibility tests were lower than 3％. The recoveries of ginsenoside Rg1， ginsenoside Re and astilbin were 96.02％-101.00％ （RSD＝1.66％，n＝6）， 98.04％- 100.50％ （RSD＝0.98％， n＝6） and 94.18％-100.55％ （RSD＝2.41％， n＝6）. RSDs of durability tests were all lower than 3％. CONCLUSIONS： Estbalished standard can be used for the quality control of Glycoprival huayu granules.