OBJECTIVE： To optimize the preparation technology of Total iridoid glycosides liposome of Cornus officinalis， and to characterize it. METHODS： Total iridoid glycosides liposome of C. officinalis was prepared by reverse phase evaporation method. The contents of loganin and morroniside in Total iridoid glycosides liposome of C. officinalis were determined by HPLC. Using encapsulation rate of total saponins （total contents of loganin and morroniside） as index， main technology factors for the preparation of Total glycosides liposome of C. officinalis were screened by single factor test； the ratio of drug to phospholipid （drug-lipid ratio）， ratio of choline to phospholipid （choline-lipid ratio）， pH of hydration medium were optimized by central composite design-response surface methodology. The Zeta potential and particle size of liposome prepared by optimal prescription were determined by using Darwin laser particle size analyzer. The morphology of liposome was observed by TEM. RESULTS： The optimal prescription included that drug-lipid ratio was 0.026； cholesterol-lipid ratio was 0.107； pH of hydration medium was 5.78. The average encapsulation efficiency of liposome was 11.63％ （n＝3）， the relative error of prediction value 11.48％ is 1.29％， the average particle size was （80.87±0.27） nm， and Zeta potential was （－13.60±5.32） mV. It was spherical or spherical-like. CONCLUSIONS： The optimized technology of Total iridoid glycosides liposome of C. officinalis is simple， and prepared liposome has small particle size.