OBJECTIVE： To analyse characteristic chromatograms of Mongolian Compound Shudage-4. METHODS： HPLC method was adopted. The determination was performed on Agilent Extend C18 with mobile phase consisted of 0.4％ acetic acid solution-methanol （gradient elution） at the flow rate of 0.6 mL/min. The detection wavelength was set at 286 nm， and column temperature was 30 ℃. The sample size was 20 μL. The in vivo and in vitro characteristic chromatograms of allsquare of Compound Shudage-4 and single ingredients were analysed and compared respectively. RESULTS： Totally 47 peaks were identified from in vitro characteristic chromatograms of allsquare of Compound Shudage-4. There were 24 common peaks from in vivo characteristic chromatograms，among which 18 ingredients corresponding to common peaks 1， 4， 7-9， 12， 14， 18-20， 22， 25， 38-41， 44， 46 were direct blood components in vitro； peaks 1， 4， 7， 8， 12， 14， 18-22， 25， 40 and 46 of serum test sample were from Alpinia officinarum； peaks 14， 18， 19， 22， 44 were from Aucklandia lappa； peaks 1， 4， 7-9， 12， 14， 18， 19， 22 were from Acorus tatarinowii. CONCLUSIONS： Blood components and some metabolites may beallsquare of Compound Shudage-4. The method can be used for the analysis of characteristic chromatograons of it.