OBJECTIVE： To establish HPLC fingerprint of Bletilla striata， and conduct cluster analysis. METHODS： HPLC method was adopted. The determination was performed on Diamonsil C18 column with mobile phase consisted of 0.1％ phosphoric acid solution-acetonitrile （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 270 nm， and column temperature was 30 ℃. The sample size was 10 μL. Using 1， 4-two [4-（glucose oxy） benzyl]-2-isobutyl malate as reference， HPLC chromatograms of 10 batches of medicinal samples were determined. Similarity evaluation was conducted by using Similarity Evaluation System for Chromatographic Fingerprint of TCM （2004 A edition）. Common peaks were determined， and cluster analysis was also conducted for it by using SPSS 23.0 statistic software. RESULTS： There were 15 common peaks in 10 batches of samples， and the similarities of 9 batches were more than 0.90. After validation， HPLC chromatograms of 10 batches of medicinal material were all in agreement with control fingerprint. The 10 batches of samples could be clustered into 3 categories. S1， S2， S3， S4， S6， S8， S9， S10 were clustered into category 1； S7 was clustered into category 2； S5 was clustered into category 3. CONCLUSIONS： Established fingerprint and the cluster analysis results can provide reference for the quality control of B. striata.