OBJECTIVE： To establish HPLC fingerprint of Tujia medicine Toddalia asiatica. METHODS： HPLC method was adopted. The determination was performed on SinoChrom ODS-BP with mobile phase consisted of acetonitrile-0.75％ glacial acetic acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was 275 nm， and column temperature was 30 ℃. The sample size was 10 μL. Using bergenin as reference， HPLC chromatograms of 10 batches of samples were determined. Similarity evaluation was performed by using Similarity Evaluation System for Fingerprint Chromatogram of TCM （2004 A）. RESULTS： There were 14 common peaks in HPLC chromatograms of 10 batches of samples， and the similarity of 4 batches of samples was higher than 0.90. CONCLUSIONS： Established fingerprint can provide reference for the quality control of T. asiatica.