OBJECTIVE： To study the effects of cinnamon oil in different drug-loaded forms on the transdermal absorption in vitro of strychnine and brucine in total alkaloids from Strychni nux-vomica. METHODS： Taking isolated skin of rats as experimental material， Franz diffusion cell was adopted. HPLC method was adopted for 48 h cumulative permeation quantity （Q48 h）， permeation rate costant （Jss）， enhancement ratio （ER） and cumulative permeation rate （T） of strychnine and brucine in total alkaloids from S. nux-vomica. The effects of cinnamon oil in different drug-loaded forms （solution， microemulsion， liposome， transfersome and alcohol transfersome agent） on transdermal absorption of strychnine and brucine in total alkaloids from S. nux-vomica were investigated and also compared with total alkaloids from S. nux-vomica without cinnamon oil. RESULTS： Q48 h of strychnine in forms of solution， microemulsion， liposomes， transfersome and alcohol transfersome were 334.44±7.65， 265.33±7.54， 249.12±6.60， 202.24±24.55， 274.30±17.12 μg/cm2， and Jss of were them 25.07， 21.62， 19.73， 17.31， 24.86 μg/（cm2·h）， respectively； ER were 5.2， 4.5， 4.1， 3.6， 5.1， respectively； T were 55.12％， 43.73％， 41.06％， 33.33％， 45.22％. Q48 h of brucine were 395.37±2.90， 285.13±6.69， 298.26±12.54， 272.26±13.32， 338.70±9.41 μg/cm2， and Jss of brucine were 32.91， 23.80， 26.04， 22.76， 29.26 μg/（cm2·h）， respectively； ER were 2.0， 1.5， 1.6， 1.4， 1.8； T were 25.54％， 18.42％， 19.27％， 17.59％， 21.88％. Compared with without cinnamon oil， adding cinnamon oil could significantly increase the Q48 h of strychnine and brucine in total alkaloids of S. nux-vomica （P＜0.05）. CONCLUSIONS： Cinnamon oil in different drug-loaded forms can improve the transdermal absorption of strychnine and brucine in the total alkali in S. nux-vomica， especially in solution form.