OBJECTIVE： To establish the method for simultaneous contents determination of 5 kinds of acid and alkaline componentsin anti-cold compound preparation. METHODS： HPLC method was adopted. The workstation was Auto·Blend Plus software of ACQUITY Arc system. The determination was performed on Discovery® HS F5-5 column with mobile phase consisted of acid mixture-base mixture-methanol-0.3％ triethylamine （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelengths were set at 270 nm （acetaminophen，salicylamide，chlorphenamine maleate，triprolidine hydrochloride） and 299 nm （aspirin）. The column temperature was 35 ℃，the sample size was 20 μL. RESULTS： The linear ranges of acetaminophen，salicylamide，acetylsalicylic acid，chlorphenamine maleate，triprolidine hydrochloride were 26-411 μg/mL（r＝0.999 6）， 16-254        μg/mL（r＝0.999 8）， 16-748 μg/mL（r＝0.998 1）， 35-565 μg/mL（r＝0.999 8） and 25-404 μg/mL（r＝0.999 7），respectively. LOQ were 3.6， 3.1， 4.0， 9.6， 6.3 μg/mL； LOD were 1.9， 1.3， 1.4， 2.9， 2.3 μg/mL， respectively. RSDs of intermediate precision， stability and reproducibility tests were all lower than 2％. Average recoveries were 100.0％-102.0％（RSD＝0.59％，n＝9）， 95.2％-101.0％（RSD＝1.55％，n＝9）， 96.2％-99.9％（RSD＝1.24％，n＝9）， 96.2％-101.5％（RSD＝1.57％，n＝9）， 96.3％-98.9％（RSD＝0.83％，n＝9）， respectively. RSDs of durability tests were lower than 3％. CONCLUSIONS： The method is simple， accurate， precise， stable， reproducible and durable， and can be used for simultaneous contents determination of 5 kinds of acid and alkaline components in anti-cold compound preparation.