OBJECTIVE： To develop the determination method for plasma concentration of effective components in essential oil from Curcuma phaeocaulis， and to study its integrated pharmacokinetics. METHODS： Sixteen rats were given the extract of essential oil from C. phaeocaulis 1.0 g/kg （by crude drug） intragastrically； blood samples 300-400 μL from orbit were collected 0， 0.17， 0.5， 1， 2， 2.5， 3， 4， 6， 8， 10， 12， 24 h after medication. The plasma concentration of α-pinene， 1，8-cineole， borneol， β-elemene， curcumol， germacrone and curdione in rats were determined by GC-MS. The determination was performed on DB-5 capillary column， using helium as carrier gas， at the flow rate of 1.2 mL/min. The injector temperature was 270 ℃， by temperature programming， and split ratio was 20 ∶ 1. The sample size was 1 μL. The ion source was electrospray ion source. The selective reaction monitoring mode was used for the positive ion scanning in the range of m/z 20-500. Pharmacokinetic parameters of above effective components were calculated by using DAS 2.0 software. The weight coefficients were customized according to the proportion of AUC0-∞ in the sum of AUC0-∞. The integrated pharmacokinetic parameters of multiple effective components in essential oil from C. phaeocaulis were calculated. RESULTS： The linear range of α-pinene， 1，8-cineole， borneol， β-elemene， curcumol， germacrone， and curdione were 2.71-173.54， 7.76-496.88， 3.37-215.72， 21.68-1 387.50， 40.21-2 573.44， 24.84-3 179.69， 47.78-3 057.81 ng/mL， respectively （r＞0.99）. The lower limits of quantitation were 2.71， 7.76， 3.37， 21.68， 40.21， 24.84， 47.78 ng/mL， respectively. The precision， accuracy and matrix effects were in line with related requirements of quantitative analysis of biological samples. The pharmacokinetic parameters of α-pinene， 1，8-cineole， borneol， β-elemene， curcumol， germacrone， and curdione were as follows that cmax were （34.72±9.97）， （99.86±5.54）， （16.10±3.37），（248.98±86.19）， （673.75±104.15）， （2 353.64±637.83）， （2 420.04±708.51）ng/mL； tmax were （2.33±0.29）， （0.67±0.29）， （1.33±0.58）， （1.83±0.76）， （0.83±0.29）， （0.89±0.18）， （1.17±0.76）h； t1/2 were （8.64±1.46）， （8.98±1.63）， （12.43±2.88）， （19.86±4.05）， （15.63±5.50）， （14.17±4.13）， （7.14±0.67）h； AUC0-t were （189.78±89.10）， （454.74±82.43）， （100.55±8.27）， （1 067.37±216.55）， （3 154.16±405.94）， （16 501.24±663.88）， （12 524.92±3 222.10）ng·h/mL； AUC0-∞ were （229.57±93.50）， （524.32±81.67）， （146.28±10.74）， （2 092.70±416.18）， （5 388.65±661.86）， （28 198.87±4 102.62）， （14 139.35±3 109.19）ng·h/mL， respectively. After integration， the pharmacokinetic parameters were as follows that cmax was         1 880.94 ng/mL； tmax was 0.50 h； t1/2 was 11.22 h； AUC0-t was 13 050.89 ng·h/mL； AUC0-∞ was 19 015.21 ng·h/mL. CONCLUSIONS： The method can be used for the detection of plasma concentration of effective components in rats； pharmacokinetic parameters of essential oil from C. phaeocaulis after integration are greatly different from single effective component， which can provide reference for characterization of its overall pharmacokinetics.