OBJECTIVE： To investigate the effects of Qiwei qingchang capsules on symptoms and the contents of VIP，SP，MOT and CGRP in serum and colon tissue of rats with chronic functional constipation （CFC）. METHODS： Totally 60 SD rats were randomly divided into normal control group， model group， positive control group （Prucalopride succinate tablets 0.02 g/kg， by prucalopride）， Qiwei qingchang capsules low-dose， medium-dose and high-dose groups （0.8， 1.6， 3.2 g/kg， by crude drug）， with 10 rats in each group. Except for normal control group， other groups were given intragastric administration of Compound diphenoxylate tablets （10 mg/kg） to induce CFC model. After modeling， administration groups were given relevant medicine intragastrically， normal control group and model group were given constant volume of normal saline intragastrically， once a day， for consecutive 7 d. General symptoms as sign， body hair color and fecal shape， first black stool discharge time and small intestinal propulsion ratio were observed in each group. ELISA method was used to detect the contents of VIP，SP，MOT and CGRP in serum and colon tissue of rats. RESULTS： Compared with normal control group， the rats were more irritable， mentally depressed， skinless and glossy， less stool frequency and fewer grains， and coarse stool in model group and administration group. The first black stool discharge time was prolonged significantly， and small intestinal propulsion ratio， the contents of SP and MOT in serum and colon tissue were decreased significantly， while the contents of VIP and CGRP were increased significantly （P＜0.05）. Compared with model group， above symptoms of administration groups were all improved； first black stool discharge time was shortened significantly， and small intestinal propulsion ratio， the contents of SP and MOT in serum and colon tissue were increased significantly， while the contents of VIP and CGRP were decreased significantly （P＜0.05）； above indexes of Qiwei qingchang capsules low-dose and medium-dose groups were significantly worse than positive control group （P＜0.05）， while the effect of high-dose group was similar to that of positive control group （P＞0.05）. CONCLUSIONS： Qiwei qingchang capsules can relieve constipation symptoms of CFC model rats， shorten first black stool discharge time， and improve small intestinal propulsion ratio， the mechanism of which may be associated with up-regulating the contents of SP and MOT， down-regulating the contents of VIP and CGRP in serum and colon tissue.