OBJECTIVE： To optimize the extraction technology of total alkaloids from Piper longum， and to provide reference for deep development and comprehensive utilization of P. longum. METHODS： With the extraction rates of total alkaloids （the target component for piperine） as reference index， using comminution degree of medicinal materials， liquid-solid ratio， extraction time and extraction times as factors， single factor test and Box-Behnken response surface methodology were adopted to optimize the extraction technology of total alkaloids from P. longum. The validation tests were conducted for 3 times （the amounts of P. longum were 1.00， 10.00， 100.00 g）. RESULTS： The optimal extraction technology was that comminution degree of 90 mesh， 32-fold absolute ethanol， extracting for 3 times， lasting for 170 min each time. Under optimal technology， the extraction rates of total alkaloids from P. longum were 5.015， 5.028， 5.041 mg/g when the amount of P. longum were 1.00， 10.00， 100.00 g； relative errors of them to predicted value 5.025 mg/g were 0.20％， 0.06％， 0.26％， respectively. There was small difference. CONCLUSIONS： The optimal extraction technology of total alkaloids of P. longum is reasonable， stable and feasible.