OBJECTIVE： To establish a method for the content determination of chlorogenic acid， rutin， phillyrin， quercetin and glycyrrhizic acid in Sangju ganmao granules. METHODS： HPLC method was adopted. The determination was performed on WondasilTM-C18 column with mobile phase consisted of acetonitrile-0.4％ phosphoric acid solution （gradient elution） at the flow rate of 0.8 mL/min. The column temperature was set at 30 ℃， and the detection wavelength was set at 254 nm. The sample size was 5 μL. RESULTS： The linear range of chlorogenic acid， rutin， phillyrin， quercetin and glycyrrhizic acid were 0.014-0.560 μg（r＝0.999 2），0.022-0.880 μg（r＝0.999 6），0.010-0.400 μg（r＝0.999 9），0.016-0.640 μg（r＝0.999 9），0.011-0.440 μg（r＝0.999 1）， respectively. The limits of quantitation were 0.752， 0.647， 0.483， 0.372， 0.242 mg/L； the limits of detection were 0.253， 0.185， 0.157， 0.162， 0.098 mg/L. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The average recoveries were 97.4％-100.9％（RSD＝1.4％，n＝6）， 95.7％-99.0％（RSD＝1.1％，n＝6）， 95.1％-98.1％（RSD＝1.3％，n＝6）， 96.4％-99.8％（RSD＝1.4％，n＝6）， 97.8％-100.4％（RSD＝1.0％，n＝6）， respectively. CONCLUSIONS： The method is simple， precise， stable and reproducible， and can be used for simultaneous determination of chlorogenic acid， rutin， phillyrin， quercetin and glycyrrhizic acid in Sangju ganmao granules.