OBJECTIVE： To establish a method for content determination of gallic acid，eugenol，alantolatone and isoalantolactone in Tibetan medicine Jiebai pills simultaneously. METHODS： HPLC method was adopted. The separation was performed on CAPCELL PAK MGⅡ C18 column with of mobile phase composed of methanol-acetonitrile-0.2％ phosphoric acid solution （gradient elution） at the flow rate of 0.8 mL/min. The detection wavelengths were 220 nm for alantolatone and isoalantolactone， 280 nm for gallic acid and eugenol.  The column temperature was at 30 ℃， and sample size was 10 μL. RESULTS： The linear ranges of gallic acid，eugenol，alantolatone and isoalantolactone were 46.50-2 325.00， 21.28-1 064.00， 4.38-219.20 and 4.17-208.40 μg/mL， respectively （all r≥0.999 1）. The limits of detection were 0.26， 0.09， 0.28，0.35 μg/mL， and the limits of quantitation were 0.74， 0.25， 0.95， 1.06 μg/mL， respectively. RSDs of precision， stability （48 h） and reproducibility tests were all lower than 2.0％ （n＝6 or n＝7）. The recoveries were 98.5％， 97.6％， 97.1％ and 101.6％， and RSDs were 1.4％， 1.9％， 1.6％， 1.7％ （n＝6）， respectively. The content of gallic acid，eugenol，alantolatone and isoalantolactone in 6 batches of Baijie pills were 4.28-7.02， 1.09-4.90， 0.25-0.60， 0.30-0.72 mg/g， respectively. CONCLUSIONS： The established method can be used for content determination of gallic acid，eugenol，alantolatone and isoalantolactone in Tibetan medicine Jiebai pills simultaneously.