OBJECTIVE： To study the mechanism of anti-inflammatory pain effect of allicin in model mice. METHODS： Totally 50 BALB/c mice were randomly divided into model group （constant volume of 0.9％ sodium chloride solution），aspirin group （200 mg/kg），allicin high-dose，medium-dose and low-dose groups （40，20，10 mg/kg），with 10 mice in each group. They were given relevant medicine intragastrically，once a day，for consecutive 3 d. One hour after last medication，they were given intraperitoneal injection of 0.7％ glacial acetic acid solution （0.2 mL）； the times of writhing response were recorded with in 15 min after modeling. Grouping and administration were same as above； 1 h after last medication，mice were given subcutaneous injection of 1.5％ formaldehyde solution （25 μL） via left back toe； accumulative licking time of mice were recorded 0-10 min （time phaseⅠ） and 10-60 min （time phaseⅡ） after modeling. Grouping and administration were same as above； 1 h after last medication，mice were given xylene （30 μL） on both sides of right ear； the degree of auricular swelling of mice was determined 2 h after modeling. The mass difference of two ears was regarded as the degree of auricular swelling and then the inhibitory rate of swelling was calculated in mice. Totally 50 BALB/c mice were randomly divided into blank control group （constant volume of 0.9％ sodium chloride solution），model group （constant volume of 0.9％ sodium chloride solution） and allicin high-dose，medium-dose and low-dose groups （40，20，10 mg/kg），with 10 mice in each group. They were given relevant medicine intragastrically，once a day，for consecutive 3 d. Twenty minutes after last medication，mice were given subcutaneous injection of 1％ carrageenan 0.9％ sodium chloride solution （30 μL） via toe. Toe volume of mice was determined before modeling and 1，3，5 h after modeling. The difference of toe volume in mice was regarded as the degree of toe swelling. The activities of SOD and GSH-Px，contents of MDA，and level of T-AOC were determined. The expression levels of NF-κB，TNF-α and IL-1β protein were detected by Western blotting. RESULTS： Compared with model group，the times of writhing response were decreased significantly in aspirin group，allicin high-dose and medium-dose groups. The licking time of mice was shortened significantly in allicin high-dose and medium-dose group at time phaseⅠ， aspirin group，allicin high-dose and medium-dose at time phase Ⅱ. The degree of auricular swelling was decreased significantly in aspirin group and allicin high-dose group. The degree of paw swelling was decreased significantly in allicin high-dose and medium-dose groups 1，3 h after modeling， in allicin high-dose group 5 h after modeling，with statistical significance （P＜0.05 or P＜0.01）. Compared with blank control group，the SOD and GSH-Px activities，level of T-AOC in paw swelling tissue of mice were decreased significantly in model group，while MDA content，the protein expression of NF-κB，TNF-α and IL-1β were increased significantly，with statistical significance （P＜0.01）. Compared with model group，SOD and GSH-Px activities in paw swelling tissue of mice were increased， while MDA content were decreased of allicin high-dose group and the activity of T-AOC in allicin high-dose and medium-dose groups were increased significantly； the protein expression of NF-κB，TNF-α and IL-1β in paw swelling tissue of mice were decreased significantly in allicin high-dose，and medium-dose groups，with statistical significance （P＜0.05 or P＜0.01）. CONCLUSIONS： Allicin has certain anti-inflammatory pain effects，and its mechanism may be related to anti-oxidative stress and inhibition of NF-κB signaling pathway.