OBJECTIVE： To study anti-inflammatory and analgesic effects of total flavonoids extract of Zhuang medicine Premna fulva for rats/mice by transdermal administration， and to provide reference for R&D of new preparation extract of P. fulva by transdermal administration. METHODS： Mice and rats were divided into blank group （40％ Ethanol solution）， positive control group （diclofenac diethylamine， 0.390 g/kg for mice， 0.280 g/kg for rats）， total flavonoids extract of P. fulva low-dose， medium-dose and high-dose groups （0.400， 0.791， 6.330 g/kg for mice， 0.136， 0.275， 2.200 g/kg for rats， calculated by crude drug） respectively. They were given medicine by transdermal administration， once a day. Ear swelling rate of mice was determined by xylene-induced inflammation method （5 d after administration）. The inflammation method with Freund’s complete adjuvant was used to determine the degree of paw edema in rats 2， 4， 9， 15 d after inducing inflammation （18 d after medication） so as to investigate anti-inflammatory effects of total flavonoids extract of P. fulva. The times of twisting in mice was determined by acetic acid-induced writhing method after injection of acetic acid in 20 min （5 d after medication） after. Inhibitory rate of pain in mice was determined by hot plate-induced pain method 30， 60， 90 min after last administration （5 d after medication）. Potential maximal analgesia percentage of rats was determined by light tail-flick method （18 d after medication）， and the improvement of pain threshold was determined in by mice tail tenderness method （5 d after medication）. Analgesic effects of total flavonoids extract of P. fulva were evaluated comprehensively. RESULTS： Compared with blank group， the ear swelling rate in mice and paw edema of rats at different time points of inflammation were decreased significantly in total flavonoids extract of P. fulva high-dose group （P＜0.05 or P＜0.01）， while the writhing times of mice was decreased significantly （P＜0.01）； inhibitory rate of pain at different times， potential maximal analgesia percentage and the improvement of pain threshold in mice were improved significantly （P＜0.05 or P＜0.01）. The degree of paw edema in rats was decreased significantly 2， 4， 9 d after inducing inflammation in total flavonoids extract of P. fulva medium-dose group （P＜0.05 or P＜0.01）； the writhing times of mice was decreased significantly （P＜0.01）； inhibitory rate of pain and the improvement of pain threshold in mice were both increased significantly 60， 90 min after last administration （P＜0.05 or P＜0.01）. The degree of paw edema in rats was decreased significantly 2， 4， 9 d after inducing inflammation in total flavonoids of P. fulva low-dose group （P＜0.05 or P＜0.01）， while inhibitory rate of pain was increased significantly 90 min after medication （P＜0.01）. CONCLUSIONS： The total flavonoids extract of P. fulva by transdermal administration have significant anti-inflammatory and analgesic effect， with a dose-response relationship.