OBJECTIVE： To establish a method for the simultaneous determination of loganic acid and isoscoparin in Sanwei longdanhua tablets. METHODS： HPLC method was adopted. The determination was performed on Inertsil ODS-3 column with mobile phase consisted of methanol-0.2％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 240 nm， and column temperature was 30 ℃. The sample size was 10 μL. RESULTS： The linear range were 0.040 08-4.008 0 μg （r＝0.999 9） for loganic acid and 0.021 96-2.196 0 μg （r＝0.999 9） for isoscoparin. The quantitative limits were 0.160 32 and 0.087 8 ng/mL， and detection limits were 0.080 16 and 0.043 92 ng/mL. RSDs of precision， stability and reproducibility tests were all lower than 2％. The recoveries were 103.07％-104.26％（RSD＝0.52％，n＝6） and 95.57％-99.61％（RSD＝1.55％，n＝6）. CONCLUSIONS： The method is simple， accurate and suitable for simultaneous determination of loganic acid and isoscoparin in Sanwei longdanhua tablets.