OBJECTIVE： To study the effect of Baji oral solution on spermatogenesis effect and sperm motility in cyclophosphamide- induced oligospermia and asthenospermia model mice， and to investigate its mechanism. METHODS： The oligospermia and asthenospermia model was induced by intraperitoneal injection of cyclophosphamide. After modeling， 50 mice were randomly divided into model control group， Compound xuanju capsule group （positive control， 0.4 g/kg）， Baji oral solution low-dose， medium-dose and high-dose groups （3.9， 7.8， 15.6 mL/kg）， with 10 mice in each group. Other 10 normal mice were included in normal control group. Administration group was given relevant medicine intragastrically once a day； normal control group and model control group were given distilled water， the volume of administration was 20.0 mL/kg， for consecutive 28 d. After medication， spermatozoon analyzer was used to detect total number， density and activity of sperm； ELISA assay was adopted to detect content of testosterone in serum and the content of bFGF in testicles of mice； histopathological examination of testicles was observed under microscope after HE staining. RESULTS： Compared with normal control group， total number， density and activity of sperm， serum content of testosterone and the content of bFGF in testicles were decreased significantly in model control group （P＜0.05 or P＜0.01）. Under microscope， the number of spermatogenic epithelium and spermatogenic cells in testis were decreased significantly （P＜0.01）， and spermatogenic cells were loosely arranged. Compared with model control group， the activity of sperm in Compound xuanju capsule group， total number and activity of sperm in Baji oral solution groups and the density of sperm in Baji oral solution high-dose group were alse increased significantly （P＜0.05 or P＜0.01）. The serum content of testosterone and the content of bFGF in testicles were alse increased significantly in administration group （P＜0.05 or P＜0.01）. Under microscope， the number of spermatogenic epithelium in testis was increased significantly with uniform thickness （P＜0.05 or P＜0.01）， and the number of spermatogenic cells was increased significantly （P＜0.01） and arranged orderly. CONCLUSIONS： Baji oral solution shows spermatogenic effect on cyclophosphamide-induced oligospermia and asthenospermia model mice and improves sperm motility， the mechanism of which may be associated with increasing serum content of testosterone and the content of bFGF in testicles.