OBJECTIVE： To establish RP-HPLC method for simultaneous determination of astilbin and rutin in Macrosolen cochinchinensis， and to compare the contents of 2 components in medicinal material from different producing areas. METHODS： The determination was performed on SHISEIDO-SPOLAR C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid （14 ∶ 86， V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 291 nm， and column temperature was 35 ℃， the sampling volume is 10 μL.Content determination and comparative analysis of M. cochinchinensis from 6 counties of Guangxi and Guangdong were conducted. RESULTS： The linear range of astilbin and rutin were 22-440 μg/mL （r＝0.999 6） and 12-240 μg/mL （r＝0.999 1）； average recoveries were 99.44％ （RSD＝1.38％， n＝9） and 99.96％ （RSD＝1.50％， n＝9）. The contents of astilbin in M. cochinchinensis were similar between provinces/autonomous region or among cities/counties （0.862 5- 0.901 1 mg/g）； the contents of rutin were similar among cities/counties in same provinces/autonomous region but there was a great difference between provinces/autonomous region （average values were    0.431 3， 0.214 9 mg/g）. CONCLUSIONS： This method is simple， accurate， stable and reproducible for the quality control of M. cochinchinensis.