OBJECTIVE： To prepare fraxinellone standard sample，and guarantee accuracy，comparability and traceability of fraxinellone and related products. METHODS： Fraxinellone monomer was obtained by means of silica gel chromatography from dried bark of Dictamnus dasycarpus. HPLC，TLC and LC-MS were used to analyze the purity of fraxinellone monomer. Their structures were confirmed by UV，IR，MS and NMR. Homogeneity，stability and constant value of it were investigated by referring to GB/T 15000.3 standard sample. RESULTS： Results of HPLC，TLC and LC-MS showed that the purity of prepared monomer was high. No significant impurity peak or spot was found in related chromatograms. A variety of spectroscopic techniques were used to identify prepared monomeric compound as fraxinellone. Prepared fraxinellone monomer had good uniformity， there was no statistical significance in purity difference among 10 samples（P＞0.05）. The stability of prepared fraxinellone monomer was good at 0-4 ℃ for 24 months， the extended uncertainty of stability was 0.16％. The standard value of fraxinellone monomer was 99.75％ in combination constant value tests of 8 laboratories，and expanded uncertainty was 0.34％. CONCLUSIONS： The fraxinellone was successfully prepared that was conformed to the technical requirement of GB/T 15000.3， and it can be used in the content determination and testing method validation of fraxinellone related products.