OBJECTIVE： To study absorption characteristics of naringin in situ single-pass intestinal perfusion model of rats. METHODS： UPLC method was established for the content determination of naringin and naringenin in intestinal perfusion samples of rats. The in situ single-pass intestinal perfusion model of rats was adopted to investigate intestinal （duodenum， jejunum， ileum and colon） absorption and metabolic characteristics [apparent permeability coefficient （Peff）， absorptivity， metabolic rate] of naringin （10    μmol/L）. RESULTS： The linear range of naringin and naringenin were 1.25-40， 1.25-40 μmol/L （R2＝0.999 4， 0.996 6）. The detection limit were 0.5， 0.4 μmol/L， and limit of quantitation were all 1.25 μmol/L. Precision of inter-day and intra-day， recovery and stability in HBSS solution， perfusion fluid of small intestine and colon were all in line with the standard. Peff of naringin in duodenum， jejunum， ileum and colon of rats were （0.28±0.19）， （0.71±0.17）， （0.30±0.02）， （0.59±0.19）（n＝6）， without statistical significance （P＞0.05）. Absorptivities were （2.90±2.14）％，（6.38±3.61）％，（3.69±0.56）％，（6.64±2.12）％（n＝6）. Naringin could be metabolized to naringenin in 4 intestinal segments of rats， with metabolic rate of （2.98±1.51）％，（2.53±1.31）％，（2.24±1.33）％，（0.70±0.20）％ （n＝6）. The lowest absorptivity and the highest metabolic rate of naringin were occurred in the duodenum， there were statistical significance compared with colon （P＜0.05）. CONCLUSIONS： Naringin shows poor permeability and poor absorption in the intestinal tract of rats. There was no specific absorption site in the rat’s intestines for naringenin； naringin could be metabolized to naringenin in small intestine and colon， but metabolic rate of naringin in small intestine is higher than in colon.