OBJECTIVE： To study the chemical constituents in ethanol extract from the stem of Miao medicine Rubus multibracteatus. METHODS： The ethanol extract from the stem of Miao medicine R. multibracteatus was isolated and purified by silica gel column， preparative liquid chromatography and Sephadex LH-20 gel column， etc. The structure of compounds were analyzed and identified according to physicochemical properties and spectrum data （MS， hydrogen spectrum and carbon spectrum）. RESULTS： Ten compounds were isolated from the ethanol extract of R. multibracteatus stem， i.e. 5，4′-dihydroxy-8-（3，3-dimethylally）-2″ ，2″ -dimethylpyrano [5，6 ∶ 6，7] isoflavone（1），3-hydroxy-1-（4′-hydroxy-3′-methoxyphenyl）propan-1-one（2），3β-hydroxysitost-5-en-7-one（3），Lupeol（4），Coniferaldehyde（5），E-p-hydroxy-coumaric acid（6），Genistein（7），1-O-p-coumaroylglycerol（8）， Scopoletin（9）， and Kaempferol（10）. CONCLUSIONS： Compound 1-9 are isolated from the plants of R. multibracteatus for the first time， and Compound 2， 5， 8 are isolated from the plants of Rubus L. for the first time. The study lays the foundation for further development and utilization of R. multibracteatus.