OBJECTIVE： To provide reference for the quality control of Prunus mume， comprehensive detection， development and utilization of active ingredients. METHODS： Using deuterated methanol and deuterium oxide （1 ∶ 0.3， V/V） as extracting solvent， 1H-NMR fingerprints of 10 batches of P. mume from different sources were established by standard presaturated pulse sequence （ZGPR） at 25 ℃. The signal was assigned through “adding standard substance qualitation” trial， database comparison and related literature comparison. The similarity of 1H-NMR fingerprints was calculated for 10 batches of P. mume from different sources by using correlation coefficient method. The quality of medicinal material was evaluated by principal component analysis （PCA）. RESULTS： A total of 19 compounds were detected in P. mume， among which chlorogenic acid， citric acid， succinic acid， acetic acid， alanine， valine， γ-aminobutyric acid， etc. Among them， acetic acid， γ-aminobutyric acid and succinic acid were detected for the first time. Moreover， the similarity of 1H-NMR fingerprinting of 10 samples was higher than 0.9， being 0.974 in average. The results of PCA showed that accumulative contribution rate of former 2 main components was 90.3％， which was consistent with the similarity evaluation. CONCLUSIONS： The established 1H-NMR method has the advantages of embodying integrity and rapid analysis， and can provide reference for the quality control of P. mume， and comprehensive testing of active ingredients.