OBJECTIVE： To establish the method for simultaneous determination of berberine hydrochloride and baicalin in Jianpi zhixiening granules. METHODS： HPLC switching walvelength method was adopted. The determination was performed on Hypersil BDS C18 column with mobile phase consisted of methanol-0.45％ phosphoric acid solution-triethylamine （50 ∶ 49 ∶ 1，V/V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 265 nm （berberine hydrochloride） and 280 nm （baicalin）. The column temperature was set at 30 ℃， and sample size was 10 μL. RESULTS： The linear range of berberine hydrochloride and baicalin were 60.3-312.8 ng（r＝0.999 7） and 81.5-368.9 ng（r＝0.999 9）. The limits of quantitation were 0.666 8，0.774 0 ng， and the limits of detection were 0.222 6，0.258 0 ng， respectively. RSDs of intermediate precision， stability and repeatability tests were all lower than 1.0％. The recoveries were 96.48％-99.30％（RSD＝1.06％，n＝6） and 95.20％-99.39％（RSD＝1.66％，n＝6）， respectively. RSDs of durability test were all lower than 2.0％. CONCLUSIONS： The method is simple， precise， stable， reproducible， accurate and  durable. It can be used for simultaneous determination of  berberine hydrochloride and baicalin in Jianpi zhixiening granules.